Characterization of the serum low-density lipoproteins of normal and two Rhesus monkeys with spontaneous hyperbeta-lipoproteinemia

Lee, J.A.; Morris, Manford D.

doi:10.1016/0006-2944(74)90028-3

Cited by 27 publications

(5 citation statements)

References 21 publications

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“…We now recognize that this value was probably too low, and that a more appropriate figure is 3.12 X 0.21 X 106, the value obtained in the current study by use of a more accurate method for determining the molecular weight and partial specific volume. This molecular weight is in agreement with the value of 3.3 X 106 reported by Lee and Morris (1974). The new partial specific volume of n-LDL was 0.969 ± 0.003 ml/g.…”

Section: Discussionsupporting

confidence: 91%

“…The results obtained with experimentally induced hyperlipidemia in rhesus monkeys invite a comparison with the spontaneous form. Lee and Morris (1974) have observed that the molecular weight of the LDL in rhesus monkeys with spontaneous hyperbetalipoproteinemia is essentially the same as in normolipidemic monkeys. It appears that the administration of atherogenic diets leading to hypercholesterolemia is attended by structural changes in LDL not seen in either normolipidemic states or genetically determined hyperlipidemias.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Study of abnormal plasma low-density lipoprotein in rhesus monkeys with diet-induced hyperlipidemia

Fless¹,

Wissler²,

Scanu³

1976

Biochemistry

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Male rhesus monkeys were divided into three groups: five were fed a regular primate chow diet and were used as controls; four received an "average" American diet; and five a special low-fat primate chow diet supplemented with 25% coconut oil and 2% cholesterol. In all of these animals, the plasma low-density lipoproteins (LDL) were isolated by ultracentrifugal flotation between densities of 1.019 and 1.050 g/ml. The LDL of the five control monkeys had variable molecular weights, with a mean value of 3.12 +/- 0.21 X 10(6) (range: 2.92 X 10(6) to 3.45 X 10(6)), and an average partial specific volume of 0.969 +/- 0.003 ml/g; both were assessed by flotation equilibrium analysis in the analytical ultracentrifuge. In the individual animals, however, the physical properties of LDL were invariant with time. The administration of either an "average" American diet or a coconut oil-cholesterol diet was accompanied by hypercholesterolemia associated with changes in LDL which were characterized by increases in molecular weight to 3.52 +/- 0.21 X 10(6) (average of nine monkeys) and in partial specific volume to 0.973 +/- 0.002 ml/g. These changes were particularly evident when the molecular weight of LDL from monkeys in the normolipidemic state was compared with that obtained from the same monkeys during the hyperlipidemic state. Chemical analyses revealed that the particles from the hyperlipidemic animals had a relatively higher cholesteryl ester content, a slight increase in phospholipids, and a marked decrease to nearly complete absence of triglycerides. The other lipoprotein components, protein, carbohydrate, free cholesterol, and fatty acids, did not vary significantly from those of control LDL. It is concluded that the administration of atherogenic diets causes structural changes in LDL which appear to be accounted for, at least in part, by changes in the composition of the lipid moiety. The changes in physical and chemical properties noted in the LDL of rhesus monkeys with experimentally induced hypercholesterolemia contrast with the apparent structurally normal LDL from rhesus monkeys with spontaneous hypercholesterolemia reported previously.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 94%

Study of abnormal plasma low-density lipoprotein in rhesus monkeys with diet-induced hyperlipidemia

Fless¹,

Wissler²,

Scanu³

1976

Biochemistry

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“…The overall information obtained thus far suggests that rhesus LDL may have a smaller particle size than human LDL and may be less hydrated, although similar in chemical composition, but analysis from a larger sample of monkeys are needed to make this conclusion valid. Lee and Morris (1974) have also characterized rhesus LDL from normal animals. Their data, however, show some differences from our findings in terms of amino acid composition (content in glycine, valine, methionine, and isoleucine), as well as higher triglyceride and lower cholesterol values.…”

Section: Discussionmentioning

confidence: 99%

Physicochemical characterization of rhesus low density lipoproteins

Fless¹,

Scanu²

1975

Biochemistry

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The serum low density lipoprotein (LDL; p 1.019-1.050 g/ml) of the normal Macaca mulatta monkey (rhesus), kept on a low-fat Purina primate chow diet, was isolated by ultracentrifugal flotation, and its physicochemical properties were compared with those previously reported for human LDL. Rhesus LDL was found to be chemically similar to human LDL. The values for the sedimentation (S25, w-O) and diffusion (D25,w-O) coefficients were 7.09 S and 2.50 times 10- minus-7 cm-2 sec- minus-1, respectively. The intrinsic viscosity was 3.40 ml g- minus-1. The partial specific volume of rhesus LDL, determined in an Anton Paar precision density meter, was 0.960 ml g- minus-1. Molecular weights, calculated from a combination of S-O and D-O and of S-O and [n], were in agreement with the weight-average molecular weight, Mw, of 2.29 times 10-6 obtained by high-speed sedimentation equilibrium. In addition, a Z-average molecular weight, Mz, of 2.73 times 10-6 was calculated because curvature in the graphs of log c vs. r-2 indicated that rhesus LDL was heterogeneous. From the frictional ratio of 1.02, a maximum hydration of 0.1 g of H2O/g of lipoprotein was obtained. On electron micrographs, rhesus LDL appeared spherical with a mean diameter of 196 A, which was substantiated by hydrodynamic analysis.

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“…Animal models currently available for the study of the role of plasma cholesterol and lipoproteins in coronary artery disease are limited. In nonhuman primates, spontaneous hypercholesterolemia was reported without extensive characterization in two rhesus monkeys by Morris and co-workers (Morris and Fitch, 1968;Lee and Morris, 1974). More recently, a screening program led to the identification of a six-member family of rhesus monkeys, three of which exhibited persistent elevation of plasma cholesterol, LDL cholesterol, and apoB on a low fat, cholesterol-free diet, and marked hypercholesterolemia following 12 weeks on a high fat diet (Scanu et al, 1988).…”

Section: Implantation and Embryo Transfermentioning

confidence: 99%

In vitro fertilization‐embryo transfer in nonhuman primates: The technique and its applications

Wolf

Thomson

Zelinski

et al. 1990

Molecular Reproduction Devel

105

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Characterization of the serum low-density lipoproteins of normal and two Rhesus monkeys with spontaneous hyperbeta-lipoproteinemia

Cited by 27 publications

References 21 publications

Study of abnormal plasma low-density lipoprotein in rhesus monkeys with diet-induced hyperlipidemia

Study of abnormal plasma low-density lipoprotein in rhesus monkeys with diet-induced hyperlipidemia

Physicochemical characterization of rhesus low density lipoproteins

In vitro fertilization‐embryo transfer in nonhuman primates: The technique and its applications

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