Characterization of the size-fractionated biomacromolecules: Tracking their role and fate in a membrane bioreactor

Meng, Fangang; Zhou, Zhongbo; Ni, Bing‐Jie; Zheng, Xing; Huang, Guocheng; Jia, Xiaoshan; Li, Shiyu; Xiong, Ya; Kraume, Matthias

doi:10.1016/j.watres.2011.06.026

Cited by 106 publications

(53 citation statements)

References 49 publications

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“…The fluorescence excitation-emission (EEM) spectrometry was used for obtaining the information of the SMP, and the details could be found in Meng's paper (Meng et al, 2011). Excitation spectrum and emission spectrum was scanned from 220-450 nm at 5 nm increments and 250-550 nm at 5 nm increments, respectively.…”

Section: Cod Was Measured According To Standard Methodsmentioning

confidence: 99%

Effect of granular activated carbon addition on the effluent properties and fouling potentials of membrane-coupled expanded granular sludge bed process

Ding

Liang

et al. 2014

Bioresource Technology

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h i g h l i g h t sAdding GAC alleviated membrane fouling of a membrane-coupled EGSB process. It reduced the concentrations of SMP, SMP ps and SMP pr by 26.8%, 27.8% and 24.7%. It primarily reduced tryptophan proteins, aromatic proteins and fulvic substances. GAC addition mainly decreased the cake layer resistance proportion by 53.5%. a r t i c l e i n f o b s t r a c tTo mitigate membrane fouling of membrane-coupled anaerobic process, granular activated carbon (GAC: 50 g/L) was added into an expanded granular sludge bed (EGSB). A short-term ultrafiltration test was investigated for analyzing membrane fouling potential and underlying fouling mechanisms. The results showed that adding GAC into the EGSB not only improved the COD removal efficiency, but also alleviated membrane fouling efficiently because GAC could help to reduce soluble microbial products, polysaccharides and proteins by 26.8%, 27.8% and 24.7%, respectively, compared with the control system. Furthermore, excitation emission matrix (EEM) fluorescence spectroscopy analysis revealed that GAC addition mainly reduced tryptophan protein-like, aromatic protein-like and fulvic-like substances. In addition, the resistance distribution analysis demonstrated that adding GAC primarily decreased the cake layer resistance by 53.5%. The classic filtration mode analysis showed that cake filtration was the major fouling mechanism for membrane-coupled EGSB process regardless of the GAC addition.

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Section: Cod Was Measured According To Standard Methodsmentioning

confidence: 99%

Effect of granular activated carbon addition on the effluent properties and fouling potentials of membrane-coupled expanded granular sludge bed process

Ding

Liang

et al. 2014

Bioresource Technology

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“…EPS matrix of cake layers were mainly comprised of large hydrophilic substances, which contained a high proportion of polysaccharides and were larger than 450 kDa or even over 1,100 kDa. These large hydrophilic substances can originate from (i) substances in SMP rejected by membrane [18], (ii) aggregation of non-degradable molecules of bulk sludge [37], (iii) metabolites of microbe aggregates [5], (iv) inner-cellular compounds or cell body of dead cells in the layers [38].…”

Section: Role and Fate Of Eps Components In Mbr Foulingmentioning

confidence: 99%

“…Besides, liquid chromatography − organic carbon detection (LC-OCD) is often employed to acquire MW profile of organic matters in EPS [12,15]. SDS-PAGE and molecularweight-cut-off (MWCO) are also applied for molecular size separation [18]. MW distribution of SMP and EPS have been indicated to impact membrane fouling.…”

Section: Introductionmentioning

confidence: 99%

“…Substances larger 100 kDa in SMP reportedly cause severe flux decline and pore plugging [19], while MW distribution of EPS affect the stability of sludge flocs, and then play a role in membrane fouling [5]. Substances larger than 100 kDa (e.g., polysaccharides), which are mostly rejected by the membranes, will accumulate and form cake layers on membrane surface [18]. Substances smaller than 100 kDa in SMP such as humic acid-like substances which are much smaller than the average size of microfiltration membrane pores, can be rejected by these membranes [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Polarity-Molecular Weight Profile of Extracellular Polymeric Substances in a Membrane Bioreactor: Comparison between Bulk Sludge and Cake Layers

Hong

Taing

Phan

et al. 2018

J. of Wat. & Envir. Tech.

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Extracellular polymeric substances (EPS) are reported to be the major foulant in membrane bioreactor (MBR) processes. It is important to understand the EPS fractions which cause irreversible fouling to reduce operation cost and energy consumption in MBR. In this study, we developed polarity-molecular weight profiling, in which EPS components were plotted on two-dimensional matrix of its polarity and molecular weight, and applied it to investigate EPS of bulk sludge and cake layers from a labscale MBR. The EPS components were also characterized via three-dimensional excitation-emission matrix (3D-EEM) spectroscopy. The result showed that hydrophilic substances as large as 100 − 670 kDa was found only in loosely-bound EPS (LB-EPS) of bulk sludge but not in that of cake layers nor in permeate. Hydrophobic substances smaller than 20 kDa were mainly found in soluble microbial products (SMP) in bulk sludge. Hydrophilic substances larger than 670 kDa was mainly found in tightly-bound EPS (TB-EPS) of bulk sludge and in LB-and TB-EPS of cake layers. These findings suggest that, after conditioning of micropores of virgin membrane by hydrophobic substances smaller than 20 kDa in SMP, hydrophilic biopolymers as large as 100 − 670 kDa in bulk sludge clog the narrowed micropores inside membrane, causing irreversible fouling.

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“…The macromolecules or biomacromolecules substances have been widely considered as a major factor resulting in membrane fouling [34][35][36]. The MW distribution patterns of DOM samples were measured by the GFC method [37,38], as shown in Figure 4.…”

Section: Molecular Weight (Mw) Distribution Of Dommentioning

confidence: 99%

Characterization of dissolved organic matter in a dynamic membrane bioreactor for wastewater treatment

Zhang

Chu

et al. 2013

Chin. Sci. Bull.

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This paper systematically examined the characteristics of dissolved organic matter (DOM) in a dynamic membrane bioreactor (DMBR) for municipal wastewater with a laboratory-scale continuous-flow device. Experimental results showed that the system performed excellent pollutants' removal efficiencies. The increase of trans-membrane pressure (TMP) for the dynamic membrane (DM) could be divided into three stages, i.e., zero increase stage, slow increase stage and abrupt rise stage. The maximal fouling rate of the DM reached to 4.34 kPa/h in abrupt rise stage. It was observed that the polysaccharides (PS) concentration of DOM samples gradually increased from the anaerobic zone to the aerobic zone in sequence, but the proteins (PN) concentration performed an opposite trend. The DM could retain a small part of the large molecular substances (>10 kDa) in the aerobic zone. Two particular fluorescence peaks appeared in the anaerobic zone and in the anoxic zone were also found in the effluent, which illustrated the dynamic cake layer closed to the stainless steel mesh might induce an anaerobic/anoxic micro environment. Based on the three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy analysis, aromatic proteins, aromatic proteinlike substance, fulvic acid-like substances and soluble microbial by-product-like materials could be biodegraded effectively in the DMBR, and the DM could partly remove the humic acid-like substances and soluble microbial by-product-like materials.

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Characterization of the size-fractionated biomacromolecules: Tracking their role and fate in a membrane bioreactor

Cited by 106 publications

References 49 publications

Effect of granular activated carbon addition on the effluent properties and fouling potentials of membrane-coupled expanded granular sludge bed process

Effect of granular activated carbon addition on the effluent properties and fouling potentials of membrane-coupled expanded granular sludge bed process

Polarity-Molecular Weight Profile of Extracellular Polymeric Substances in a Membrane Bioreactor: Comparison between Bulk Sludge and Cake Layers

Characterization of dissolved organic matter in a dynamic membrane bioreactor for wastewater treatment

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