Characterization of the Soybean Genome Using EST-derived Microsatellite Markers

Hisano, Hiroshi; Sato, Shusei; Isobe, Sachiko; Sasamoto, Shigemi; Wada, Tsuyuko; Matsuno, Ai; Fujishiro, Tsunakazu; Yamada, Manabu; Nakayama, Shinobu; Nakamura, Yasukazu; Watanabe, Satoshi; Harada, Kyuya; Tabata, Satoshi

doi:10.1093/dnares/dsm025

Cited by 129 publications

(125 citation statements)

References 30 publications

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“…The homeologous duplicated regions were investigated by gel-blot analysis with RFLP markers (Shoemaker et al 1996;Tsubokura et al 2008) and EST-derived SSR markers (Hisano et al 2007). GmPhyA1 and -2 were located on LG I and O, and these linkage groups shared the homeologous duplicated region (Shoemaker et al 1996;Hisano et al 2007;Liu et al 2008;Tsubokura et al 2008). Information obtained from the database LIS, together with previous studies, has shown that a part of LG L and N might be homeologous.…”

Section: Discussionmentioning

confidence: 99%

Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3

et al. 2009

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Photosensitivity plays an essential role in the response of plants to their changing environments throughout their life cycle. In soybean [Glycine max (L.) Merrill], several associations between photosensitivity and maturity loci are known, but only limited information at the molecular level is available. The FT3 locus is one of the quantitative trait loci (QTL) for flowering time that corresponds to the maturity locus E3. To identify the gene responsible for this QTL, a map-based cloning strategy was undertaken. One phytochrome A gene (GmPhyA3) was considered a strong candidate for the FT3 locus. Allelism tests and gene sequence comparisons showed that alleles of Misuzudaizu (FT3/FT3; JP28856) and Harosoy (E3/E3; PI548573) were identical. The GmPhyA3 alleles of Moshidou Gong 503 (ft3/ft3; JP27603) and L62-667 (e3/e3; PI547716) showed weak or complete loss of function, respectively. High red/far-red (R/FR) long-day conditions enhanced the effects of the E3/FT3 alleles in various genetic backgrounds. Moreover, a mutant line harboring the nonfunctional GmPhyA3 flowered earlier than the original Bay (E3/E3; PI553043) under similar conditions. These results suggest that the variation in phytochrome A may contribute to the complex systems of soybean flowering response and geographic adaptation.

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Section: Discussionmentioning

confidence: 99%

Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3

et al. 2009

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“…A total 23 SSR primers derived from soybean sequences. Hisano et al [12] were used in this study. PCR analysis was performed in a total volume of 15 μl that contained 20 ng/µl of genomic DNA, 0.25 μM of each primer (forward and reverse), 0.125 mM of each dNTPs, 0.15 units of TaqDNA polymerase, and 1 × reaction buffer.…”

Section: Dna Extraction and Ssr Analysismentioning

confidence: 99%

Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Yuliasti

Reflinur

2017

Atom Indo.

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The objectives of this research were to evaluate (1) the performance of soybean mutant lines under drought stress conditions, and (2) the genetic diversity and relationship among the mutant lines using SSR markers. The field evaluation was conducted during the dry season of 2011 and 2012 at the experimental Farm of Mataram University, West Nusa Tenggara, Indonesia. The field experiment was set up in a randomized block design. Ten mutant lines and two control varieties were evaluated in four replications. Genetic distance among evaluated lines were determined based on allelic diversity analysis using 40 simple sequence repeat (SSR) loci. Under drought stress conditions, two mutant lines, Kdl3 and Kdl8, showed a better performance compared to the other ones. The high yielding mutant lines were Kdl3 and Kdl8, which yielded 1.75 t ha-1 and 1.69 t ha-1, respectively, compared to the parent and national control, Panderman 1.43 t ha-1 and Muria 1.32 t ha-1. These mutant lines required 30.75 to 32 days to flower and 79.75 to 83.75 day to harvest with relatively short plant height 28.25 and 23.35 cm respectively. Those mutant characters were better than those of the other three mutants, the original parents, and the control soybean species. Since the evaluated soybean mutant lines yielded more under drought stress conditions than the standard varieties, they can be used and registered as drought-tolerant soybean mutants. Moreover, the evaluated soybean accessions showed a wide genetic distance. The accessions were clustered into two groups according to their genetic background, namely group I (the Panderman with three mutant lines) and group II (the Muria with two mutant lines). Twenty-three out of 40 evaluated SSR loci,

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“…Recently, the usage of soybean for biodiesel has made this crop more economically important, and the whole-genome sequencing of Williams 82 (G. max) and IT182932 (G. soja) have been completed accordingly (Hisano et al, 2007;Kim et al, 2010;Schmutz et al, 2010). Yu & Kiang (1993) reported that Korea might be one of the major center of soybean origins based on electrophoretic assays of wild soybean (G. soja) and cultivated soybeans (G. max).…”

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Genetic Diversity and Population Structure of Korean Soybean Collection Using 75 Microsatellite Markers

Lee¹,

Choi²,

Yi³

et al. 2014

Korean J. Crop Sci.

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Soybean (Glycine max L.) is crucial legume crop as source of high quality vegetable protein and oil, and Korea is regarded as a part of center of soybean origin. To expand the information of conserved genetic diversity, we analyzed the genetic variability of soybean collection mainly introduced Korean accessions using 75 microsatellite markers. A total of 1,503 alleles with an average value of 20.0 alleles were detected among 644 accessions. Korean collection revealed average allele number of 13.4 while Chinese, Japanese and Southeast Asian accessions showed 9.0, 5.4 and 6.5 mean alleles, respectively. Especially, Korean accessions showed more number of private allele per locus as 3.4 contrary to other geographical groups. The mean expected heterozygosity and polymorphic information content was 0.654 and 0.616, respectively, and expected heterozygosity values were not significantly distinguished according to the geographical groups. The phylogenetic dendrogram and deduced population structure based on DNA profiles of 75 SSR loci showed Korean accessions formed distinct gene pool against Chinese accessions, and could be divided into five subpopulations. Korean soybean accessions have specific genetic diversity and might be serve the valuable alleles for bio-industry as a part of the center of soybean origin.

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Characterization of the Soybean Genome Using EST-derived Microsatellite Markers

Cited by 129 publications

References 30 publications

Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3

Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3

Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Genetic Diversity and Population Structure of Korean Soybean Collection Using 75 Microsatellite Markers

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