Characterization of the SPI-1 Type III Secretion System in Pseudomonas fluorescens 2P24

Wang, Jing; Luo, Yuan; Gu, Yunqing; Wei, Hengling

doi:10.3389/fmicb.2021.749037

Cited by 3 publications

(5 citation statements)

References 43 publications

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“…We identified that the strain XL17 against Psa and B. cinerea belongs to P. bijieensis within the P. corrugata subgroup and the well-studied DAPG-producing strain 2P24 previously classified into species P. fluorescens [54,55,65,66] belongs to a novel genomospecies mostly closely-related to P. bijieensis. P. bijieensis is a recently identified novel species based on the antifungal strain L22-9 T isolated from cornfield soil [67].…”

Section: Discussionmentioning

confidence: 99%

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Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit

Ali

Luo²,

Zhang

et al. 2022

Microorganisms

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Kiwifruit worldwide suffers from the devastating diseases of bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) and gray mold caused by Botrytis cinerea. Here, an endophytic bacterium XL17 isolated from a rape crown gall was screened out for its potent antagonistic activities against Psa and B. cinerea. Strain XL17 and its cell-free culture filtrate (CF) inhibited the growth of Psa and B. cinerea, Psa-associated leaf necrosis, and B. cinerea-associated kiwifruit necrosis. Electron microscopy showed that XL17 CF could damage the cell structures of Psa and B. cinerea. Genome-based taxonomy revealed that strain XL17 belongs to Pseudomonas bijieensis within the P. corrugata subgroup of the P. fluorescens species complex. Among the P. corrugata subgroup containing 31 genomospecies, the presence of the phl operon responsible for the biosynthesis of the phenolic polyketide 2,4-diacetylphloroglucinol (DAPG) and the absence of the lipopeptide/quorum sensing island can serve as the genetic marker for the determination of a plant-protection life style. HPLC detected DAPG in extracts from XL17 CF. MALDI-TOF-MS analysis revealed that strain XL17 produced cyclic lipopeptides of the viscosin family and orfamide family. Together, phenotypic, genomic, and metabolic analyses identified that P. bijieensis XL17 producing DAPG and cyclic lipopeptides can be used to control bacterial canker and gray mold pathogens of kiwifruit.

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Section: Discussionmentioning

confidence: 99%

“…P. bijieensis and strain 2P24 contain two types (Hrp1 and SPI-1) of T3SS [2,54,55]. P. bijieensis but not strain 2P24 contains the single "orphaned" T3SS effector gene hopAA (Figure 6).…”

Section: Genes Associated With Plant-interaction Life Style In Pseudo...mentioning

confidence: 99%

Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit

Ali

Luo²,

Zhang

et al. 2022

Microorganisms

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“…Bacteria in the biofilm form an interactive community and are not free planktic cells (Cvitkvitch et al, 2003). It has been reported that pcoI positively regulated the formation of biofilm in P. fluorescens 2P24, and rpoZ positively regulated the transcription expression of pcoI (Wang et al, 2021). Therefore, we tested the regulatory effect of rpoZ gene on biofilm.…”

Section: Determination Of Biocontrol Characteristic Of Rpoz In Pseudo...mentioning

confidence: 99%

“…Motility tests were conducted as described by Rashid and Kornberg (2000), with slight modification (Wang et al, 2021). For swimming tests, we used water medium that contained 0.2% agar.…”

Section: Motility Testsmentioning

confidence: 99%

RpoZ regulates 2,4-DAPG production and quorum sensing system in Pseudomonas fluorescens 2P24

Wei

Dong

et al. 2023

Front. Microbiol.

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IntroductionPseudomonas fluorescens 2P24 was isolated from soil of natural decay associated with wheat take-all and it can effectively control soil-borne diseases caused by a variety of plant pathogens. 2,4-diacetylphloroglucinol (2,4-DAPG), is produced by P. fluorescens 2P24 and plays an important role in the prevention and control of plant diseases. To understand the resistant mechanism, in this study, we conducted experiments to explore the regulation role of rpoZ in the synthesis of the antibiotic 2,4-DAPG and regulation of QS system.MethodsA random mini-Tn5 mutagenesis procedure was used to screen regulators for phlA transcription in stain PM901, which containing a phlA∷lacZ transcriptional fusion reporter plasmid. We identified 12 insertion mutants could significantly change phlA gene expression. By analyzing the amino acid sequences of the interrupted gene, we obtained a mutant strain Aa4-29 destroyed the rpoZ gene, which encodes the omiga subunit. We constructed the plasmid of rpoZ mutant (pBBR-△rpoZ) transformed into competent cells of P. fluorescens 2P24 by electro-transformation assay. The strains of P. fluorescens 2P24/pBBR, 2P24-△rpoZ/pBBR, 2P24-△rpoZ/pBBR-rpoZ were used to evaluate the regulation role of rpoZ in 2,4-DAPG production and quorum sensing system.ResultsAccording to β-galactosidase activity, we found that rpoZ positively regulated the expression of phlA (a synthesis gene of 2,4-DAPG) and PcoI (a synthesis gene of PcoI/PcoR QS signal system) at the transcriptional level. The production of 2,4-DAPG antibiotic and signal molecule AHL was influenced by rpoZ. Further, rpoZ was involved in regulating rsmA expression. RpoZ also has a certain regulatory effect on rpoS transcription, but no effect on the transcription of phlF, emhABC and emhR. According to the biocontrol assay, P. fluorescens 2P24 strains with rpoZ showed obvious antagonism ability against the Rhizoctonia solani in cotton, while the mutant strain of rpoZ lost the biocontrol effect. RpoZ had a significant effect on the swimming and biofilm formation in P. fluorescens 2P24.ConclusionOur data showed that rpoZ was an important regulator of QS system, 2,4-DAPG in P. fluorescens 2P24. This may imply that P. fluorescens 2P24 has evolved different regulatory features to adapt to different environmental threats.

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“…Mutation of the rscC gene did not affect the biocontrol property, although it abolished the secretion of effectors ( Liu et al, 2016 ). Recently, it has been shown that mutation in the transcriptional regulators (hilA and invF) of 2P24 T3SS led to decrement in resistance against Fusarium graminearum (2P241invF), increased biofilm formation (2P241hilA), reduced chemotactic responses (2P241hilA, 2P241invF, and 2P241invE-C), and reduced reactive oxygen species’ production (2P241invE-C) more than wild type strain 2P24 ( Wang et al, 2021 ). P. fluorescens strains WH6 and KD contain a complete and functional T3SS showing homology with the T3SS region of P. syringae.…”

Section: Secretion System In Pgprs: An Overviewmentioning

confidence: 99%

Secretory molecules from secretion systems fine-tune the host-beneficial bacteria (PGPRs) interaction

Gupta,

Chauhan,

Jha

et al. 2024

Front. Microbiol.

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Numerous bacterial species associate with plants through commensal, mutualistic, or parasitic association, affecting host physiology and health. The mechanism for such association is intricate and involves the secretion of multiple biochemical substances through dedicated protein systems called secretion systems SS. Eleven SS pathways deliver protein factors and enzymes in their immediate environment or host cells, as well as in competing microbial cells in a contact-dependent or independent fashion. These SS are instrumental in competition, initiation of infection, colonization, and establishment of association (positive or negative) with host organisms. The role of SS in infection and pathogenesis has been demonstrated for several phytopathogens, including Agrobacterium, Xanthomonas, Ralstonia, and Pseudomonas. Since there is overlap in mechanisms of establishing association with host plants, several studies have investigated the role of SSs in the interaction of plant and beneficial bacteria, including symbiotic rhizobia and plant growth bacteria (PGPB). Therefore, the present review updates the role of different SSs required for the colonization of beneficial bacteria such as rhizobia, Burkholderia, Pseudomonas, Herbaspirillum, etc., on or inside plants, which can lead to a long-term association. Most SS like T3SS, T4SS, T5SS, and T6SS are required for the antagonistic activity needed to prevent competing microbes, including phytopathogens, ameliorate biotic stress in plants, and produce substances for successful colonization. Others are required for chemotaxis, adherence, niche formation, and suppression of immune response to establish mutualistic association with host plants.

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Characterization of the SPI-1 Type III Secretion System in Pseudomonas fluorescens 2P24

Cited by 3 publications

References 43 publications

Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit

Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit

RpoZ regulates 2,4-DAPG production and quorum sensing system in Pseudomonas fluorescens 2P24

Secretory molecules from secretion systems fine-tune the host-beneficial bacteria (PGPRs) interaction

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