Characterization of the structure and analysis of the anti-oxidant effect of microalga Spirulina platensis polysaccharide on Caenorhabditis elegans mediated by modulating microRNAs and gut microbiota

Chen, Yihan; Wan, Xuzhi; Wu, Desheng; Ouyang, Yuezhen; Gao, Luying; Chen, Zhengxin; El‐Seedi, Hesham R.; Wang, Mingfu; Chen, Xinhua; Zhao, Chao

doi:10.1016/j.ijbiomac.2020.09.041

Cited by 40 publications

(17 citation statements)

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“…The bonding structures and structural formula of PAP-1 are shown in Figure 5 G,H, respectively. Chen et al showed that the monosaccharides of PAP were mainly composed of rhamnose, fucose, arabinose, xylose, mannose, and glucose with the molar ratio of 3.42:0.76:0.34:0.53:0.43:0.59 [ 5 ]. The results of Pr et al showed that PAP contained a relatively large proportion of galactose [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

“…PAP, as a natural health product, has no toxic and side effects and can be taken for a long time. PAP is a type of cyanobacteria, which are considered to be one of the most potent species for prevention and nutritious supplementary because of their abundant special active ingredients [ 5 ]. At present, researchers on PAP have made progress in the separation, purification, and determination of chemical structure and biological activity, but there are still many problems to be solved.…”

Section: Introductionmentioning

confidence: 99%

“…At present, researchers on PAP have made progress in the separation, purification, and determination of chemical structure and biological activity, but there are still many problems to be solved. Most studies have been based on crude polysaccharides, but crude polysaccharides may contain multiple mixed sugar components and small molecular impurities, so it is difficult to obtain a definitive conclusion from these studies [ 6 , 7 , 8 ] In addition, there have been some studies on the structure of isolated and purified PAP by FT-IR, NMR, and GC-MS, but research on the structure has not been thorough enough [ 5 , 9 , 10 ]. The detailed structure of polysaccharides was the foundation for studying their potential mechanisms and structure-activity relationships.…”

Section: Introductionmentioning

confidence: 99%

“…With the continuous development of sequencing technology, RNA-Seq has become an effective tool for excavating and identifying new transcripts. The above studies indicate that PAP has antiviral and antioxidant effects [ 5 , 8 , 9 ], and PRV infection RAW264.7 cells may cause the differential expression of circRNA, miRNA, and mRNA [ 29 , 30 , 31 ]. In this study, the non-coding RNA sequencing data of PRV-infected RAW264.7 cells and PAP-1 acting on PRV-infected RAW264.7 cells from the GEO database were analyzed, and the circRNA-miRNA-mRNA network was successfully constructed, which has the characteristics of highlighting specific molecular functions and mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

et al. 2021

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To investigate the structure of Arthrospira platensis polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE-52 chromatography column and Sephacryl S-200 high-resolution gel column to obtain PAP-1. The antioxidant activity and regulation of PAP-1 on PRV-infected RAW264.7 cells of circRNA-miRNA-mRNA network were investigated by chemical kit, Q-PCR, and ce-RNA seq. The results indicated that the molecular weight (Mw) of PAP-1, which was mainly composed of glucose and eight other monosaccharides, was 1.48 × 106 Da. The main glycosidic bond structure of PAP-1 was →4)-α-D-Glcp-(1→. PAP-1 may be increased the antioxidant capacity by regulating the circRNA-miRNA-mRNA network in PRV-infected RAW264.7 cells. This study provided a scientific foundation for further exploring the antioxidant activity of PAP-1 based on its structure.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

et al. 2021

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“…Plant (wheat and French bean) bio-stimulant [60] Nostoc sphaeroides Food and health industries [6] Spirulina platensis Functional food [51] Porphyridium sp. Thickening/lubrication agent [8] Recently, due to the great worldwide concern, researchers have been looking for environmentally friendly solutions for the bioremediation of different wastewater contaminants.…”

Section: Potential Application Referencementioning

confidence: 99%

Microalgae Polysaccharides: An Overview of Production, Characterization, and Potential Applications

et al. 2021

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Microalgae and cyanobacteria are photosynthetic microorganisms capable of synthesizing several biocompounds, including polysaccharides with antioxidant, antibacterial, and antiviral properties. At the same time that the accumulation of biomolecules occurs, microalgae can use wastewater and gaseous effluents for their growth, mitigating these pollutants. The increase in the production of polysaccharides by microalgae can be achieved mainly through nutritional limitations, stressful conditions, and/or adverse conditions. These compounds are of commercial interest due to their biological and rheological properties, which allow their application in various sectors, such as pharmaceuticals and foods. Thus, to increase the productivity and competitiveness of microalgal polysaccharides with commercial hydrocolloids, the cultivation parameters and extraction/purification processes have been optimized. In this context, this review addresses an overview of the production, characterization, and potential applications of polysaccharides obtained by microalgae and cyanobacteria. Moreover, the main opportunities and challenges in relation to obtaining these compounds are highlighted.

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Three different oils improve HFD‐induced lipid metabolism disorders via driving gut microbiota

Lai,

Liu,

Han

et al. 2023

Euro J Lipid Sci & Tech

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Schizochytrium sp. L. oil (SO), fish oil (FO), and sacha inchi oil (SIO) can regulate high‐fat diet‐induced obesity, but the effect on gut microbiota and the mechanism remain unclear. This study aimed to find out the different effects of the three oils mediated the gut microbiota on lipid metabolism. In comparison with the high‐fat diet (HFD) group, SO, FO, and SIO could reduce fat mass accumulation and decrease the levels of the serum and liver biochemical parameters in mice. The regulatory effect on gut microbiota in mice is determined by 16S rRNA gene high‐throughput sequencing. Caulobacter, Acetatifactor, and Mucispirillum as dominant bacterial genus are enriched in intervention groups. SO, SIO, and FO could distinctly decrease interleukin 6, nuclear factor‐kappa B, tumor necrosis factor‐alpha, sterol regulatory element‐binding protein 1c, FAS, and HMGCR expressions in liver, upregulate the expression of acyl‐coenzyme A oxidase 1 and A acetyltransferases and regulate the thermogenesis. SO, SIO, and FO has a comprehensive impact on lipid metabolism by the expression of genes related to fat synthesis, cholesterol synthesis, inflammation, energy consumption, the structure of intestinal flora, abundance of beneficial bacteria, and intestinal diversity. Based on these results, SO, SIO, and FO may be used as functional food to drive beneficial changes in gut microbiota and then regulate the lipid metabolism disorders.Practical Applications: Obesity‐related diseases caused by lipid metabolism disorders have driven the rapid development of the diet drug market. However, the side effects of diet drugs on the human body also make the majority of consumers worried. Natural oil products that regulate lipid metabolism disorders and dietary obesity provide a substitute. Schizochytrium sp. L. oil (SO), fish oil (FO), and sacha inchi oil (SIO) are rich in high‐quality unsaturated fatty acids and are not suitable for high temperature cooking but can be eaten in low temperature cooking and sauces and so on. SO, FO, and SIO can be used to develop functional food that could reduce lipid metabolism disorders.

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Characterization of the structure and analysis of the anti-oxidant effect of microalga Spirulina platensis polysaccharide on Caenorhabditis elegans mediated by modulating microRNAs and gut microbiota

Cited by 40 publications

References 67 publications

Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

Microalgae Polysaccharides: An Overview of Production, Characterization, and Potential Applications

Three different oils improve HFD‐induced lipid metabolism disorders via driving gut microbiota

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