Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All

Abstract

Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng (P. huashania)-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. Sequential genomic in situ hybridization (GISH) and multicolor fluorescence in situ hybridization (mc-FISH) analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D. Expressed sequence tag-sequence tagged site (EST-STS) marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line. In addition, the H139 line was shown to be cytologically stable with a dwarf phenotype and increased spikelet number. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding.

Take-All Disease: New Insights into an Important Wheat Root Pathogen

“…The genetic and mechanistic basis of this resistance is not known. This species has previously been used to introgress genetic loci conferring resistance to powdery mildew and leaf rust [33,34] Psathyrostachys huashania Keng (2n = 14, NsNs) is a wild wheat relative that also demonstrates high levels of resistance to take-all, and has successfully been introgressed into a wheat background [35]. A wheat-P. huashania substitution line has identified chromosome 2N as having resistance properties through molecular analysis, and this source could be used to improve wheat breeding for resistance to multiple diseases.…”

Identification of a Wheat-Psathyrostachys huashanica 7Ns Ditelosomic Addition Line Conferring Early Maturation by Cytological Analysis and Newly Developed Molecular and FISH Markers

“…The pedigree provided the only evidence that DT23 carried novel Eps gene(s) from the P. huashanica 7Ns chromosome. All seven Ns chromosomes of P. huashanica have been verified to be useful on account of the vast number of beneficial genes carried: Stripe rust resistance genes are located on 1Ns, 2Ns, 3Ns, 4Ns, and 5Ns ( Du et al, 2014a , c , d ; Li et al, 2019 ; Qu et al, 2021 ); leaf rust resistance genes are located on 1Ns and 7Ns ( Du et al, 2013c , 2014b ); powdery mildew resistance genes are located on 1Ns, 3Ns, 4Ns, and 5Ns ( Han et al, 2020 Li et al, 2020a 2021a Liu et al, 2021 ); a take-all resistance gene is located on 2Ns ( Bai et al, 2020 ); gluten and gliadin-related genes are located on 1Ns and 6Ns ( Du et al, 2013b ; Qu et al, 2021 ); and early maturity-related genes are located on 6Ns ( Wang et al, 2015 ). The present study is the first report of new Eps gene(s) probably associated with a group 7 chromosome of P. huashanica .…”

“…Subsequently, derivative lines with P. huashanica chromosome(s) incorporated into the common wheat background were generated, such as an amphiploid line (PHW-SA, 2 n =8 x =56, AABBDDNsNs; Kang et al, 2009 ), and chromosome addition and substitution lines ( Zhao et al, 2004 , 2010 ; Kishii et al, 2010 ). In the last decade, a series of wheat- P. huashanica derivative lines were developed and identified using molecular cytological methods, including wheat- P. huashanica 1Ns-7Ns disomic addition lines ( Du et al, 2013a , b , c , 2014a , b , c , d ), 1Ns(1D), 2Ns(2D), 3Ns(3D), and 5Ns(5D) disomic substitution lines ( Li et al, 2019 2021a Bai et al, 2020 ; Qu et al, 2021 ), and several translocation lines ( Kang et al, 2016 ; Li et al, 2020a ; Liu et al, 2021 ). These progeny lines outperformed their wheat parents with regard to disease resistance and agronomic traits, demonstrating that P. huashanica is a superior wild relative useful to wheat breeding programs.…”

Development and Molecular Cytogenetic Identification of a New Wheat–Psathyrostachys huashanica Keng Translocation Line Resistant to Powdery Mildew

“…To utilize the desirable traits of P. huashanica , distant crosses have been performed between P. huashanica and wheat since the 1980s ( Chen et al, 1991 ). A series of wheat– P. huashanica -derived lines, including addition lines, substitution lines, translocation lines, and intergeneric amphiploids, have been developed and identified by molecular cytogenetic methods ( Wang and Shang, 2000 ; Cao et al, 2008 ; Kang et al, 2008 , 2016 ; Du et al, 2010 , 2013a , b , c , 2014 ; Li et al, 2019 , 2020 ; Bai et al, 2020 ). These derived lines with single P. huashanica chromosomes incorporated into the wheat genome exhibited better agronomic performance than their wheat parents, indicating that P. huashanica can be used as a valuable source of disease resistance and of several useful agronomic traits for wheat improvement.…”