Characterization of theEgeria densa leaf symplast: Response to plasmolysis, deplasmolysis and to aromatic amino acids

Erwee, M. G.; Goodwin, Peter

doi:10.1007/bf01281693

Cited by 75 publications

(31 citation statements)

References 14 publications

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“…Siniilarly, Drake et ai. (1978) found that intercellular electricai coupling was restored some 6 h after plasmolysis of oat coleoptile cells, considerably longer than the time scale of recovery reported by Erwee & Goodwin (1984).…”

Section: What Happens To Plasmodesmata ?mentioning

confidence: 89%

“…found that in oat coleoptile cells, physical continuity of the symplast may not always be disrupted by severe plasmolysis, despite the fact that functional continuity is interrupted. Restoration of functional continuity between cells following deplasmolysis was elegantly demonstrated by Erwee & Goodwin (1984). They injected fluorescent probes of fixed molecular weights into the cytosol of Elodea cells.…”

Section: What Happens To Plasmodesmata ?mentioning

confidence: 99%

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Plasmolysis: new insights into an old process

Oparka¹

1994

New Phytologist

134

124

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SUMMARY Early microscopists made detailed observations of the phenomenon of plasmolysis, many of which have passed into the historical record without comment. This review considers the significance of these observations in the light of recent cell‐biological studies which are addressing the complex relationships between the plasma membrane (PM) and the extracellular matrix, as well as the interrelationships of the PM, endomembrane system and cytoskeleton. Emphasis is placed on the relative roles of Hechtian strand formation and PM vesiculation as mechanisms allowing the conservation of PM during a plasmolysis/deplasmolysis cycle. The behaviour of plasmodesmata during plasmolysis is considered in relation to the ability of cells to resume intercellular transport following deplasmolysis. Finally, the behaviour of cells during freeze‐induced ptesmolysis is addressed.

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Section: What Happens To Plasmodesmata ?mentioning

confidence: 89%

Section: What Happens To Plasmodesmata ?mentioning

confidence: 99%

Plasmolysis: new insights into an old process

Oparka¹

1994

New Phytologist

134

124

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“…Movement of tracer dyes has been shown to be controlled by changes in cellular concentrations of ions, such as Ca2' and aromatic amino acids (3, 4). In addition, size exclusion limits have been established for symplastic movement in some tissues (5, 12, 13, 30) which can apparently be overcome by treatments, such as plasmolysis (4). In other studies, barriers to symplastic movement have been found even in regions where apparently normal plasmodesmata are present, suggesting the existence of symplastic "domains" within specific plant tissues (5).…”

mentioning

confidence: 99%

“…Current experimental evidence suggests that the free movement of solutes through the symplast is regulated at the level of the plasmodesmata (3)(4)(5). The actual mechanisms controlling movement through plasmodesmata are unknown at present, but modulation ofintracellular Ca levels is implicated (3).…”

mentioning

confidence: 99%

Symplastic Transport in Ipomea tricolor Source Leaves

Madore

Oross²,

Lucas³

1986

Plant Physiol.

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A novel method for the delivery of the fluorescent dye Lucifer Yellow CH to the cytosol of a source leaf mesophyll cell was devised which utilized a preencapsulation of the dye in phospholipid vesicles (liposomes). The liposomes were easily injected into the vacuoles of leaf cells of Beta vulgaris or Ipomea tricolor, where fusion with the tonoplast resulted in the release of the dye into the cytosol. Subsequent cell-to-cell movement of the dye was readily followed by fluorescence microscopy. Using this liposome technique symplastic continuity from the the mesophyll to the minor veins of the source leaf of Ipomea tricolor was demonstrated. This agreed with ultrastructural studies which demonstrated the presence of plasmodesmata between all cells from the mesophyll to the minor veins. The symplastic movement of dye from the injected mesophyll cell to the minor veins was unaffected by pretreatment of the leaf tissues with 2 millimolar p-chloromercuribenzenesulfonic acid. Pretreatment of the leaf tissues at alkaline pH (3-IN-morpholinol propanesulfonic acid-KOH, pH 8.0) had no apparent effect on dye movement between adjacent mesophyll cells but inhibited the movement of dye into and along the minor veins. Thus, although there were no apparent barriers to symplastic solute movement in this leaf, symplastic barriers could be imposed by the experimental conditions used.

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“…-la plasmolyse des parenchymes qui altère les liaisons symplastiques, altération concretisee par l'immobilité des sondes fluorescentes (Erwee et Goodwin, 1984), n'inhibe pas le chargement des nervures, mais au contraire le stimule (Hagège et coll., 1984); -le PCMBS, un inhibiteur de thiol non perméant, bloque de façon efficace l'absorption du saccharose par les nervures foliairel:l (Giaquinta, 1976 ;Delrot et coll., 1980). Son mode d'action est connu.…”

Section: 2-fonctionsunclassified

Physiologie comparée des cellules compagnes du phloème et des cellules associées aux vaisseaux

Bonnemain¹,

Fromard²

1987

Bulletin de la Société Botanique de France. Actualités Botaniqu

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Characterization of theEgeria densa leaf symplast: Response to plasmolysis, deplasmolysis and to aromatic amino acids

Cited by 75 publications

References 14 publications

Plasmolysis: new insights into an old process

Plasmolysis: new insights into an old process

Symplastic Transport in Ipomea tricolor Source Leaves

Physiologie comparée des cellules compagnes du phloème et des cellules associées aux vaisseaux

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