Characterization of Thermophilic Halotolerant Aeribacillus pallidus TD1 from Tao Dam Hot Spring, Thailand

Abstract

The bacterial strain TD1 was isolated from Tao Dam hot spring in Thailand. Strain TD1 was Gram positive, rod-shaped, aerobic, motile, and endospore forming. The cell was 2.0–40 μm in length and about 0.4 μm in diameter. The optimum growth occurred at 55–60 °C and at pH 7–8. Strain TD1 was able to grow on medium containing up to 10% NaCl. The DNA G+C content was 38.9 mol%. The cellular fatty acid content was mainly C16:0, which comprised 25.04% of the total amount of cellular fatty acid. 16S rDNA showed 99% identity to Aeribacillus pallidus DSM 3670T. Bayesian tree analysis strongly supported the idea that strain TD1 is affiliated with genus Aeribacillus, as Aeribacillus pallidus strain TD1. Although the 16S rDNA of A. pallidus strain TD1 is similar to that of A. pallidus DSM 3670T, some physiological properties and the cellular fatty acid profiles differ significantly. A. pallidus strain TD1 can produce extracellular pectate lyase, which has not been reported elsewhere for other bacterial strains in the genus Aeribacillus. A. pallidus strain TD1 may be a good candidate as a pectate lyase producer, which may have useful industrial applications.

Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

“…The Thar Desert soil is an evidence of ecological niches of Aeribacillus pallidus as the strain 2BS showed the closeness to type strain KCTC3564 of Aeribacillus pallidus. Most of the strains of genus Aeribacillus isolated from hot springs, geothermal oil field and desert soils 48,49 . The strain Aeribacillus pallidus SAT4 was isolated from Thar Desert, Pakistan used to produce antibacterial peptide 50 .…”

Genome Sequence of Aeribacillus pallidus Strain GS3372, an Endospore-Forming Bacterium Isolated in a Deep Geothermal Reservoir

“…The type strain (strain H12) was isolated from thermally treated sewage, and its full genome is not publicly available. More strains of this species have been isolated from hot springs ( 4 , 5 ), production water from an oil reservoir ( 6 ), and crude-contaminated soil ( 7 ). Strain GS3372 was isolated multiple times from fluid samples (50 to 70°C) from the deep geothermal reservoir of Gross Schoenebeck, in the North German Basin (52°54′13.15″ N, 13°36′5.43″ E).…”

The Chemical Characteristic and Microbial Diversity of the Hot Spring at Phusang National Park

“…Thermus, a genus found in Thailand produced an extracellular protease (Kanasawud et al, 1992). In the Tao Dam hot spring, the TD1 bacterial strain may be a good candidate as a pectate lyase producer (Yasawong et al, 2011). A neopullulanase-like enzyme from Bor Khleung hot spring (Tang et al, 2008) and lipolytic enzymes potential can be used in industrial applications (Tirawongsaroj et al, 2008).…”