Characterization of third generation cephalosporin-resistant Escherichia coli clinical isolates from Ushuaia, Argentina

Gramundi, Ivan; Albornoz, Ezequiel; Boutureira, Manuel; Rapoport, Melina; Gómez, Sonia; Corso, Alejandra; Castro, Gabriel Granado; Faccone, Diego

doi:10.1016/j.ram.2022.06.002

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…Interestingly, two of these strains (E. coli ECO 37 isolated in 2014 and E. coli ECO 81 isolated in 2015) were isolated before the first description of the mcr-1 gene in isolates from animals, food, and patients in China (Liu et al, 2016). Escherichia coli ST615 was not identified in those epidemiological studies and in other studies performed with E. coli strains from Argentina isolated from the clinic or other environments before or after the COVID-19 pandemic (Sennati et al, 2012;Dominguez et al, 2019;Faccone et al, 2023;Gramundi et al, 2023;Piekar et al, 2023), confirming the sporadic condition of this clone. Escherichia coli M19736 ST615 strain isolated in 2015 was identified at that time as one of the first isolates harboring the mcr-1 gene in human infections caused by E. coli in Latin America (Rapoport et al, 2016).…”

Section: Discussionmentioning

confidence: 72%

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Carrera Páez,

Olivier,

Gambino

et al. 2024

Front. Cell. Infect. Microbiol.

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There is scarce information concerning the role of sporadic clones in the dissemination of antimicrobial resistance genes (ARGs) within the nosocomial niche. We confirmed that the clinical Escherichia coli M19736 ST615 strain, one of the first isolates of Latin America that harbors a plasmid with an mcr-1 gene, could receive crucial ARG by transformation and conjugation using as donors critical plasmids that harbor blaCTX-M-15, blaKPC-2, blaNDM-5, blaNDM-1, or aadB genes. Escherichia coli M19736 acquired blaCTX-M-15, blaKPC-2, blaNDM-5, blaNDM-1, and aadB genes, being only blaNDM-1 maintained at 100% on the 10th day of subculture. In addition, when the evolved MDR-E. coli M19736 acquired sequentially blaCTX-M-15 and blaNDM-1 genes, the maintenance pattern of the plasmids changed. In addition, when the evolved XDR-E. coli M19736 acquired in an ulterior step the paadB plasmid, a different pattern of the plasmid’s maintenance was found. Interestingly, the evolved E. coli M19736 strains disseminated simultaneously the acquired conjugative plasmids in different combinations though selection was ceftazidime in all cases. Finally, we isolated and characterized the extracellular vesicles (EVs) from the native and evolved XDR-E. coli M19736 strains. Interestingly, EVs from the evolved XDR-E. coli M19736 harbored blaCTX-M-15 though the pDCAG1-CTX-M-15 was previously lost as shown by WGS and experiments, suggesting that EV could be a relevant reservoir of ARG for susceptible bacteria. These results evidenced the genetic plasticity of a sporadic clone of E. coli such as ST615 that could play a relevant transitional link in the clinical dynamics and evolution to multidrug/extensively/pandrug-resistant phenotypes of superbugs within the nosocomial niche by acting simultaneously as a vector and reservoir of multiple ARGs which later could be disseminated.

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Section: Discussionmentioning

confidence: 72%

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Carrera Páez,

Olivier,

Gambino

et al. 2024

Front. Cell. Infect. Microbiol.

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“…E. coli strains belonging to the O101:H10 serotype and CC10 were previously isolated from human bloodstream in Spain (ESBL phenotype) [114], UTI in Taiwan (carbapenemase phenotype) [115], and Argentina (ESBL phenotype) [116].…”

Section: Discussionmentioning

confidence: 99%

First Report and Characterization of the mcr-1 Positive Multidrug-Resistant Escherichia coli Strain Isolated from Pigs in Croatia

Kompes,

Duvnjak,

Reil

et al. 2023

Microorganisms

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The emergence and rapid spread of the plasmid-mediated colistin-resistant mcr-1 gene introduced a serious threat to public health. In 2021, a multi-drug resistant, mcr-1 positive Escherichia coli EC1945 strain, was isolated from pig caecal content in Croatia. Antimicrobial susceptibility testing and whole genome sequencing were performed. Bioinformatics tools were used to determine the presence of resistance genes, plasmid Inc groups, serotype, sequence type, virulence factors, and plasmid reconstruction. The isolated strain showed phenotypic and genotypic resistance to nine antimicrobial classes. It was resistant to colistin, gentamicin, ampicillin, cefepime, cefotaxime, ceftazidime, sulfamethoxazole, chloramphenicol, nalidixic acid, and ciprofloxacin. Antimicrobial resistance genes included mcr-1, blaTEM-1B, blaCTX-M-1, aac(3)-IId, aph(3’)-Ia, aadA5, sul2, catA1, gyrA (S83L, D87N), and parC (A56T, S80I). The mcr-1 gene was located within the conjugative IncX4 plasmid. IncI1, IncFIB, and IncFII plasmids were also detected. The isolate also harbored 14 virulence genes and was classified as ST744 and O101:H10. ST744 is a member of the ST10 group which includes commensal, extraintestinal pathogenic E. coli isolates that play a crucial role as a reservoir of genes. Further efforts are needed to identify mcr-1-carrying E. coli isolates in Croatia, especially in food-producing animals to identify such gene reservoirs.

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Characterization of third generation cephalosporin-resistant Escherichia coli clinical isolates from Ushuaia, Argentina

Cited by 2 publications

References 13 publications

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

First Report and Characterization of the mcr-1 Positive Multidrug-Resistant Escherichia coli Strain Isolated from Pigs in Croatia

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