Characterization of Thirteen Date Palm(Phoenix dactylifera L.) Cultivars by Enzyme Electrophoresis using the PhastSystem

Booij, I.; Monfort, S.; Ferry, Michel

doi:10.1016/s0176-1617(11)81847-x

Cited by 15 publications

(6 citation statements)

References 15 publications

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“…Fortunately, the revealed SSR alleles were successfully used to molecularly discriminate a nearly unlimited number of date palm cultivars. Compared to other studies of date palms, the scored percentage of resolution is higher than those observed using isoenzymes (Booij et al, 1995;Salem et al, 2001) and plastid DNA haplotypes (Sakka, 2003). These authors reported 69, 93, and 71% resolving power, respectively.…”

Section: Resultscontrasting

confidence: 59%

Genetic diversity of Qatari date palm using SSR markers

Elmeer

Mattat²

2015

Genet. Mol. Res.

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ABSTRACT. The genetic diversity in the date palm germplasm of 59 female accessions representing 12 cultivars from different locations in Qatar was investigated using 14 loci of simple-sequence repeat (SSR) primers. A total of 94 alleles, with a mean of 6.7 alleles per locus, were scored. The number of alleles per locus varied from 3 (primer mPdCIR090) to 11 (primers mPdCIR010 and mPdCIR015). The amplified SSR band sizes ranged from 104 to 330 bp. The mean gene diversity was 0.66 and ranged from 0.39 (locus mPdCIRO93) to 0.86 (locus mPdCIR015), indicating that the Qatari date palm collection has a high degree of genetic diversity. The heterozygosity ranged from 0 (marker mPdCIR090) to 98% (marker mPdCIR010). Forty-four percent of the variability is explained at the inter-population level, while 56% of the variability is maintained within individuals. However, the loci mPdCIR044, mPdCIR057, mPdCIR090, and mPdCIR093 revealed that the total gene diversity is explained at the inter-population level. The Qatari populations Khalas, Shishi, Barhi, Hillali, Khnaizi, Gar, and Jabri showed significant differentiation compared to all other populations. The average fixation index was 0.24814, showing that about 24.81% of 1625 Genetic diversity of Qatari date palm ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 1624-1635 (2015) the genetic variation was present among populations, which correlated with analysis of molecular variance.

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Section: Resultscontrasting

confidence: 59%

Genetic diversity of Qatari date palm using SSR markers

Elmeer

Mattat²

2015

Genet. Mol. Res.

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“…This study is more precise and accurate compared to other date palm studies, i.e. plastid DNA halophytes (Sakka 2003 ) and isozymes (Salem et al 2001 ; Booij et al 1995 ). These data proved that SSR markers are more influential, powerful and key identifiers of date palm sex for speeding up the breeding programs.…”

Section: Resultsmentioning

confidence: 59%

Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers

et al. 2016

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Microsatellite markers containing simple sequence repeats (SSRs) are a valuable tool for genetic analysis. Date palm is a dioecious and slow flowering and is very difficult to identify the gender of the trees until it reaches the reproductive age (5–10 years). A total of 12 microsatellite primers were used with 30 date palm samples, 14 parents (8 male + 6 females) and 16 progeny (developed from parents breeding) which showed that microsatellites were highly polymorphic, having a great number of alleles. A total of 124 alleles were characterized in 12 SSR loci. On average, there are 9.08 alleles per locus, with a range from 5 to 16 alleles, for primers mpdCIR15 and mpdCIR57, respectively. These primers produced 15 polymorphic loci specifically in male date palm samples and the seedlings harboring the unique fragments were further characterized as male plants. Increasingly, 38.46 % of these loci were scored as homozygous alleles while 61.53 % heterozygous allelic loci were determined. Primer mpdCIR48 produced a specific locus (250/250) in all male samples whereas the same locus was absent in female samples. Similarly, a locus of 300/310 bp reoccurred in 5 date palm male samples using marker DP-168 which indicated that these are the promising candidate marker to detect the sex in date palm seedlings at early stage. The data resulted from combination of 12 primers enabled the 16 seedling samples progeny (developed from parents breeding) of date palm cultivars to divide into two groups i.e., male and female regarding their sex expression comparative to the parents (male + female) using the principle coordinate analysis.

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“…Fortunately, the revealed SSR alleles were successfully used to molecularly discriminate a nearly unlimited number of date‐palms cultivars. Compared to studies reported in date‐palms, the scored percentage of resolution is higher than observed using isoenzymes (B ooij et al 1995; O uld M ohamed S alem et al 2001) and plastid DNA haplotypes (S akka 2003). These authors have reported 69%, 93% and 71% resolving power values respectively.…”

Section: Discussionmentioning

confidence: 58%

Genetic diversity of Tunisian date palms (Phoenixdactylifera L.) revealed by nuclear microsatellite polymprohism

et al. 2005

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Fourteen microsatellite loci of Phoenix dactylifera were targeted to examine the genetic diversity in Tunisian date-palms germplasm. They showed a high level of polymorphism in 49 accessions from three main oases with little geographic structure within Tunisia. The microsatellite data agrees with previous analyses of Tunisian germplasm using other molecular markers. 100% of local date-palms accessions were successfully fingerprinted and easily distinguished by the help of only three loci. The possibility of using microsatellites for large scale molecular labelling of offshoots and in vitro plantlets and their implication in the plant material certification is discussed.

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Characterization of Thirteen Date Palm(Phoenix dactylifera L.) Cultivars by Enzyme Electrophoresis using the PhastSystem

Cited by 15 publications

References 15 publications

Genetic diversity of Qatari date palm using SSR markers

Genetic diversity of Qatari date palm using SSR markers

Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers

Genetic diversity of Tunisian date palms (Phoenixdactylifera L.) revealed by nuclear microsatellite polymprohism

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