Characterization of Three Rifamycins via Electrospray Mass Spectrometry and HPLC-Thermospray Mass Spectrometry

Abstract

Rifamycins are a class of antibiotic compounds of which rifampicin is the most commonly prescribed. Conventional electron-impact mass spectrometry of rifampicin has not been found to provide useful data. Thermospray and electrospray mass spectrometry are studied as potential tools for the analysis of rifampicin, rifamycin SV, and rifamycin B. Using thermospray and electrospray ionization, all three compounds provide significant ion intensity for either the [MH]+ or [MNa]+ ions. In addition, combined high-performance liquid chromatography-thermospray mass spectrometry provides useful analytical data for a mixture of the three rifamycins.

A rapid and sensitive HPLC–MS method for the detection of plasma and cellular rifampicin

“…HPLC-MS allows for added specificity for the detection of rifampicin compared to that obtained with HPLC. In a previous publication [10], rifampicin was detected by HPLC-MS following direct injection of the drug and identical ions (m/z: [MH] + , [MNa] + and [MH-CH 3 OH] + ) were seen in the present study where rifampicin was extracted from plasma. None of the rifampicin ions were detected at any time in extracted drug free plasma samples.…”

“…To date, there are a number of published analytical methods [2][3][4][5][6][7][8][9][10], some that have been used to determine plasma rifampicin concentrations, but none used to determine cell associated rifampicin. Further, previous publications often have not been used on plasma samples [3,8,10], have not used and internal control [2][3][4][5], or have limited sensitivity [8,9]. Here a validated, accurate, precise and sensitive HPLC-MS method has been developed for the detection of rifampicin in both plasma and cells.…”

“…Ideally, the assay should have quick sample processing and be able to measure low rifampicin concentrations. A number of HPLC-based assays for rifampicin have been described [2][3][4][5][6][7][8][9][10], but these have lacked standardisation. For some assays an internal standard was not included [2][3][4][5], or the assays were not suitable for analysis of plasma (lacking an work-up procedure) [6,7] or else required large volumes of sample with relatively low sensitivity [8,9].…”

“…For some assays an internal standard was not included [2][3][4][5], or the assays were not suitable for analysis of plasma (lacking an work-up procedure) [6,7] or else required large volumes of sample with relatively low sensitivity [8,9]. Only one previous HPLC-MS based assay has been reported [10], and lack of internal standard and validated workup procedure does not make this suitable for analysing drug concentrations in plasma or cells.…”

A Rapid, Sensitive and Validated Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry Method for Determination of Rifampicin in Rat Plasma: Application to Pharmacokinetic Study

“…Now, considering several analytical methods available for determination of RIF in biological fluids and pharmaceutical dosage forms, including high-performance thin layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), UPLC, LC-MS/MS and matrix-assisted laser desorption/ionization with a time of flight (MALDI-TOF). But, none of the methods is predominant and having various drawbacks viz less sensitivity, complex extraction procedure, and large sample size and unsatisfactory quantitative analysis [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27].…”