Characterization of Tissue Scaffolds Using Synchrotron Radiation Microcomputed Tomography Imaging

Duan, Xiaoman; Li, Naitao; Chen, Daniel; Zhu, Ning

doi:10.1089/ten.tec.2021.0155

Cited by 17 publications

(11 citation statements)

References 106 publications

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“…With regards to in-vivo studies, conventional micro-CT has been the most widely used method to track bone regeneration although the synchrotron radiation micro-CT provides benefits including images with greater quality, resolution, contrast, shorter scan time as well as non-destructive 3D visualization ( Cooper et al, 2011 ). Synchrotron radiation micro-CT has been illustrated promising to perform in-vivo imaging to track tissue regeneration once scaffolds are implanted in live animals over duration of study ( Izadifar et al, 2016 ; Duan et al, 2021 ). This state-of-the-art approach is missing in most BTE studies although it can provide researchers with useful information in terms of scaffold degradation rate, the way implant is integrated with the host tissue, vascularization, and bone formation over time.…”

Section: Bone Structure and Defectsmentioning

confidence: 99%

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Yazdanpanah

Johnston

Cooper

et al. 2022

Front. Bioeng. Biotechnol.

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Treating large bone defects, known as critical-sized defects (CSDs), is challenging because they are not spontaneously healed by the patient’s body. Due to the limitations associated with conventional bone grafts, bone tissue engineering (BTE), based on three-dimensional (3D) bioprinted scaffolds, has emerged as a promising approach for bone reconstitution and treatment. Bioprinting technology allows for incorporation of living cells and/or growth factors into scaffolds aiming to mimic the structure and properties of the native bone. To date, a wide range of biomaterials (either natural or synthetic polymers), as well as various cells and growth factors, have been explored for use in scaffold bioprinting. However, a key challenge that remains is the fabrication of scaffolds that meet structure, mechanical, and osteoconductive requirements of native bone and support vascularization. In this review, we briefly present the latest developments and discoveries of CSD treatment by means of bioprinted scaffolds, with a focus on the biomaterials, cells, and growth factors for formulating bioinks and their bioprinting techniques. Promising state-of-the-art pathways or strategies recently developed for bioprinting bone scaffolds are highlighted, including the incorporation of bioactive ceramics to create composite scaffolds, the use of advanced bioprinting technologies (e.g., core/shell bioprinting) to form hybrid scaffolds or systems, as well as the rigorous design of scaffolds by taking into account of the influence of such parameters as scaffold pore geometry and porosity. We also review in-vitro assays and in-vivo models to track bone regeneration, followed by a discussion of current limitations associated with 3D bioprinting technologies for BTE. We conclude this review with emerging approaches in this field, including the development of gradient scaffolds, four-dimensional (4D) printing technology via smart materials, organoids, and cell aggregates/spheroids along with future avenues for related BTE.

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Section: Bone Structure and Defectsmentioning

confidence: 99%

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Yazdanpanah

Johnston

Cooper

et al. 2022

Front. Bioeng. Biotechnol.

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“…Owing to the clinical relevance of CT imaging, it is also a technique utilised to analyse tissue engineering constructs for CNS repair. For example, the visualisation of radiopaque hydrogels can be acquired through CT scans due to the variable absorption of X-rays (ionising radiation) by different tissues in the body [210]. CT imaging can demonstrate the microstructure and degradation profile of implanted biodegradable scaffolds implanted in the brain and spinal cord of mice [211,212].…”

Section: Computed Tomography (Ct)mentioning

confidence: 99%

Emerging scaffold- and cellular-based strategies for brain tissue regeneration and imaging

et al. 2022

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Stimulating brain tissue regeneration is a major challenge after central nervous system (CNS) injury, such as those observed from trauma or cerebrovascular accidents. Full regeneration is difficult even when a neurogenesis-associated repair response may occur. Currently, there are no effective treatments to stimulate brain tissue regeneration. However, biomaterial scaffolds are showing promising results, where hydrogels are the materials of choice to develop these supportive scaffolds for cell carriers. Their combination with growth factors, such as brain-derived neurotrophic factor (BDNF), basic fibroblast growth factor (bFGF), or vascular endothelial growth factor (VEGF), together with other cell therapy strategies allows the prevention of further neuronal death and can potentially lead to the direct stimulation of neurogenesis and vascularisation at the injured site. Imaging of the injured site is particularly critical to study the reestablishment of neural cell functionality after brain tissue injury. This review outlines the latest key advances associated with different strategies aiming to promote the neuroregeneration, imaging, and functional recovery of brain tissue. Graphical abstract

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“…Raman scattering-based techniques are gradually emerging to compete with their fluorescence-based counterparts [8] . Another non-destructive method for imaging of complex 3D architectures is synchrotron-based micro-CT [9] . An ideal strategy here may involve a multi-parametric assay that allows studying the model from different perspectives and in a non-invasive manner, over multiple time points.…”

Section: Challengesmentioning

confidence: 99%

“…Others combine microfluidic components that simulate the blood flow and ensure an automatic refreshment of the media. However, more sophisticated printed models have integrated a vascularization system within the 3D cell culture, by combining sacrificial printing with microfluidics [9] . This approach is so far, the most advanced method and closely simulates the vascular system of the native tissue.…”

Section: Challengesmentioning

confidence: 99%

3D printed scaffolds: Challenges toward developing relevant cellular in vitro models

Molina-Martínez¹,

Liz‐Marzán²

2022

Biomaterials and Biosystems

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Characterization of Tissue Scaffolds Using Synchrotron Radiation Microcomputed Tomography Imaging

Cited by 17 publications

References 106 publications

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Emerging scaffold- and cellular-based strategies for brain tissue regeneration and imaging

3D printed scaffolds: Challenges toward developing relevant cellular in vitro models

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