Characterization of tissue-specific biomarkers with the expression of circRNAs in forensically relevant body fluids

Liu, Baonian; Song, Feng; Yang, Qinrui; Zhou, Yuxiang; Shao, Chengchen; Shen, Yiwen; Zhao, Ziqin; Tang, Qiqun; Hou, Yiping; Xie, Jianhui

doi:10.1007/s00414-019-02027-y

Cited by 46 publications

(26 citation statements)

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“…As a result, circRNAs are highly stable and have extended half-lives Cell Reports 33, 108552, December 29, 2020 5 Resource ll OPEN ACCESS compared to linear mRNAs (Li et al, 2018b). circRNAs have been reported to be present in numerous human tissues (Vo et al, 2019) and in a few biofluids, such as saliva (Bahn et al, 2015), blood (Memczak et al, 2015), semen (Liu et al, 2019) and urine (Kö lling et al, 2019;Vo et al, 2019). A direct comparison of the circRNA read fraction between biofluids and tissues is currently lacking in literature.…”

Section: Figure 1 Study Flow Chartmentioning

confidence: 99%

“…As such, most studies have explored the abundance of individual mRNAs in one specific biofluid by qRT-PCR (Herring et al, 2018;Maker et al, 2019;Marzioni et al, 2015;Oreo et al, 2014;Tian et al, 2016;Welch et al, 2016). circRNAs have been reported in saliva (Bahn et al, 2015), semen (Liu et al, 2019), blood (Li et al, 2018a), and urine (Kö lling et al, 2019;Vo et al, 2019). Recently, the mRNA content of plasma and serum has been investigated using dedicated sequencing approaches such as phospho-RNA-seq, small input liquid volume extracellular RNA sequencing (SILVER-seq), and the SMARTer Stranded Total RNA-Seq Kit method (Everaert et al, 2019;Giraldez et al, 2019;Metzenmacher et al, 2020;Zhou et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Charting Extracellular Transcriptomes in The Human Biofluid RNA Atlas

et al. 2020

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Section: Figure 1 Study Flow Chartmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Charting Extracellular Transcriptomes in The Human Biofluid RNA Atlas

et al. 2020

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“…As a result, circRNAs are highly stable and have extended half-lives compared to linear mRNAs. 27 CircRNAs have been reported to be present in numerous human tissues 23 and in a few biofluids such as saliva 20 , blood 28 , semen 21 and urine 23,24 . A direct comparison of the circRNA read fraction between biofluids and tissues is currently lacking in literature.…”

Section: Circular Rnas Are Enriched In Biofluids Compared To Tissuesmentioning

confidence: 99%

“…As such, most studies have explored the abundance of individual mRNAs in one specific biofluid by RT-qPCR [13][14][15][16][17][18][19] . CircRNAs have been reported in saliva 20 , semen 21 , blood 22 and urine 23,24 . Recently, the mRNA content of plasma and serum has been investigated using dedicated sequencing approaches like Phospho-RNA-Seq and SILVERseq 25,26 .…”

Section: Introductionmentioning

confidence: 99%

Charting extracellular transcriptomes in The Human Biofluid RNA Atlas

Hulstaert

Morlion

Cobos

et al. 2019

Preprint

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Extracellular RNAs present in biofluids have emerged as potential biomarkers for disease.Where most studies focus on plasma or serum, other biofluids may contain more informative RNA molecules, depending on the type of disease. Here, we present an unprecedented atlas of messenger, circular and small RNA transcriptomes of a comprehensive collection of 20 different human biofluids. By means of synthetic spike-in controls, we compared RNA content across biofluids, revealing a more than 10 000-fold difference in RNA concentration. The circular RNA fraction is increased in nearly all biofluids compared to tissues. Each biofluid transcriptome is enriched for RNA molecules derived from specific tissues and cell types. In addition, a subset of biofluids, including stool, sweat, saliva and sputum, contains high levels of bacterial RNAs. Our atlas enables a more informed selection of the most relevant biofluid to monitor particular diseases. To verify the biomarker potential in these biofluids, four validation cohorts representing a broad spectrum of diseases were profiled, revealing numerous differential RNAs between case and control subjects. Taken together, our results reveal novel insights in the RNA content of human biofluids and may serve as a valuable resource for future biomarker studies.

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“…Although these early studies clearly document the existence of circRNA molecules, their potential impact was underappreciated. With the advent of some advanced RNA sequencing techniques and methods for calculating nonpolyadenylate RNA transcription, more and more circRNAs have been found in paleontological, nematode, zebrafish, mouse, and human cells (Zhang et al, 2014), and regulation of circRNA levels can lead to a variety of molecular and physiological phenotypic changes, which include effects on growth and development (Liu B. et al, 2019), the nervous system (Irie et al, 2019), innate immunity, microRNAs (Yang et al, 2017), and many disease-related pathways (Du et al, 2018). As a result, circRNA became a hot spot.…”

Section: Overview Of Circrnas the Discovery Of Circrnasmentioning

confidence: 99%

The Roles of CircRNAs in Regulating Muscle Development of Livestock Animals

Yang

Chen

2021

Front. Cell Dev. Biol.

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The muscle growth and development of livestock animals is a complex, multistage process, which is regulated by many factors, especially the genes related to muscle development. In recent years, it has been reported frequently that circular RNAs (circRNAs) are involved widely in cell proliferation, cell differentiation, and body development (including muscle development). However, the research on circRNAs in muscle growth and development of livestock animals is still in its infancy. In this paper, we briefly introduce the discovery, classification, biogenesis, biological function, and degradation of circRNAs and focus on the molecular mechanism and mode of action of circRNAs as competitive endogenous RNAs in the muscle development of livestock and poultry. In addition, we also discuss the regulatory mechanism of circRNAs on muscle development in livestock in terms of transcription, translation, and mRNAs. The purpose of this article is to discuss the multiple regulatory roles of circRNAs in the process of muscle development in livestock, to provide new ideas for the development of a new co-expression regulation network, and to lay a foundation for enriching livestock breeding and improving livestock economic traits.

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Characterization of tissue-specific biomarkers with the expression of circRNAs in forensically relevant body fluids

Cited by 46 publications

References 46 publications

Charting Extracellular Transcriptomes in The Human Biofluid RNA Atlas

Charting Extracellular Transcriptomes in The Human Biofluid RNA Atlas

Charting extracellular transcriptomes in The Human Biofluid RNA Atlas

The Roles of CircRNAs in Regulating Muscle Development of Livestock Animals

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