Characterization of toxin-producing strains of Dinophysis spp. (Dinophyceae) isolated from French coastal waters, with a particular focus on the D. acuminata-complex

Sechet, Véronique; Sibat, Manoëlla; Billien, Gwenael; Carpentier, Liliane; Rovillon, Georges-Augustin; Raimbault, Virginie; Malo, Florent; Gaillard, Sylvain; Perrière-Rumèbe, Myriam; Heß, Philipp; Chomérat, Nicolas

doi:10.1016/j.hal.2021.101974

Cited by 16 publications

(15 citation statements)

References 69 publications

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“…led to an annual loss of 1.37 million £ (GBP) [91]. Thus, further experiments with cultured strains can lead to an improved understanding of the variability in toxin production between the strains from the same and different geographic locations as well as the influence of changes in physical-chemical variables [69,92].…”

Section: Discussionmentioning

confidence: 99%

Growth, Toxin Content and Production of D<em>inophysis norvegica</em> in Cultured Strains Isolated From Japan

Nagai¹,

Basti²,

Uchida³

et al. 2023

Preprint

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This study is the first report of the successful cultivation of Dinophysis norvegica isolated from Japanese coastal waters (7 of 48 isolated cells were established as the clonal cultures, 14.5%) and their toxin content and production. The strains were maintained at relatively high abundance (>2,000 cells mL-1) for more than one year when fed on Mesodinium rubrum with the addition of Teleaulax amphioxeia. At the end of the one-month incubation, the total amounts of pectenotoxin-2 (PTX2), dinophysistoxin-1 (DTX1) and okadaic acid (OA) were 132.0-375.0 ng mL-1 (n = 7), 0.7-3.6 ng mL-1 (n = 3) and trace level OA (n = 1), respectively. Similarly, the cell quota of PTX2 and DTX1 were 60.6-152.4 pg cell-1 (n = 7) and 0.5-1.2 pg cell-1 (n = 3), respectively. These data indicated that toxin production varies depending on the strains in this species. In the growth experiment, D. norvegica grew, but it was very slow first 12 days. However, after that they grew exponentially with the maximum growth rate of 0.56 divisions day-1 (during Day 24-27), reaching a maximum concentration of 3,000 cells mL-1 at the end of incubation (Day 36), suggesting that they have a long lag phase. In the toxin production, OA was not at a detectable level (≤ 0.010 ng mL-1) during the 36 days of incubation except for Day 6. The concentration of DTX1 and PTX2 showed similar patterns as described for vegetative growth, but the toxin production still increased on Day 36 (1.3 ng mL-1 and 154.7 ng mL-1 in DTX1 and PTX2, respectively). The findings of this study provide novel information on the toxin content and production in D. norvegica as well as details on the culturing and maintenance of the species.

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Section: Discussionmentioning

confidence: 99%

Growth, Toxin Content and Production of D<em>inophysis norvegica</em> in Cultured Strains Isolated From Japan

Nagai¹,

Basti²,

Uchida³

et al. 2023

Preprint

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“…Other Dinophysis species, such as D. acuminata, a species genetically close to D. cf. sacculus (Séchet et al 2021), have been shown to occur in a wide range of salinities. Indeed, in Chile, D. acuminata has been found at salinities between 5 to 31 (Diaz et al, 2011) and at salinities 8 to 27 in North East USA (Hattenrath-Lehmann and Gobler, 2015), reflecting an important tolerance to salinity fluctuations.…”

Section: Discussionmentioning

confidence: 99%

“…Three strains of D. acuminata-complex isolated from the French Atlantic coast at Loscolo (Vilaine Bay, strain IFR-DSA-01Lo = Dsa-Lo) and Meyran (Arcachon Bay, strain IFR-DSA-01Me = Dsa-Me) and one strain from Thau Lagoon (Crique de l'Angle, strain IFR-DSA-02Th = Dsa-Th) in the Mediterranean Sea were used. Genetic characterization of these strains has been realized by Séchet et al, (2021) who investigated morphology and genetics and showed that they all clustered in D. acuminata-complex subclade in the cox1 phylogenetic analysis, corresponding presumably to D. cf. sacculus (Séchet et al, 2021).…”

Section: Culture Maintenancementioning

confidence: 99%

“…Genetic characterization of these strains has been realized by Séchet et al, (2021) who investigated morphology and genetics and showed that they all clustered in D. acuminata-complex subclade in the cox1 phylogenetic analysis, corresponding presumably to D. cf. sacculus (Séchet et al, 2021).…”

Section: Culture Maintenancementioning

confidence: 99%

“…D1/D2 regions of the LSU or ITS part of the rRNA operon) or mitochondrial (cox1) genes are closely related and do not support discrimination into several species (Wolny et al, 2020). Indeed, although D. sacculus seems to have a different ecological niche compared to D. acuminata, its taxonomical identity is not clear and genetic data (LSU, ITS rRNA or cox1 genes) could not separate it clearly from other species of the complex (Séchet et al 2021). Indeed, D. sacculus found on European Atlantic coasts and in the Mediterranean Sea, is likely to perform well in closed or semi-enclosed areas, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Effect of a short-term salinity stress on the growth, biovolume, toxins, osmolytes and metabolite profiles on three strains of the Dinophysis acuminata-complex (Dinophysis cf. sacculus)

Gaillard

Réveillon²,

Danthu³

et al. 2021

Harmful Algae

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Molecular networking as a novel approach to unravel toxin diversity of four strains of the dominant Dinophysis species from French coastal waters

et al. 2021

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Characterization of toxin-producing strains of Dinophysis spp. (Dinophyceae) isolated from French coastal waters, with a particular focus on the D. acuminata-complex

Cited by 16 publications

References 69 publications

Growth, Toxin Content and Production of D<em>inophysis norvegica</em> in Cultured Strains Isolated From Japan

Growth, Toxin Content and Production of D<em>inophysis norvegica</em> in Cultured Strains Isolated From Japan

Effect of a short-term salinity stress on the growth, biovolume, toxins, osmolytes and metabolite profiles on three strains of the Dinophysis acuminata-complex (Dinophysis cf. sacculus)

Molecular networking as a novel approach to unravel toxin diversity of four strains of the dominant Dinophysis species from French coastal waters

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