Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp. The GenBank accession number for the 33803 bp sequence of Tn1549 is AJ192329.

Garnier, Fabien; Taourit, Séad; Glaser, Philippe; Courvalin, Patrice; Galimand, Marc

doi:10.1099/00221287-146-6-1481

Cited by 154 publications

(106 citation statements)

References 43 publications

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“…For pIP501, the most closely related putative type IV secretion proteins are all encoded by pRE25 (e.g., Orf10 is 99% identical to Orf33 of pRE25). The pIP501 orf10 product is also 27% identical to the orf16 product encoded by the E. faecalis conjugative transposon Tn1549 (79). On the recently completed E. faecalis V583 genome sequence, another putative VirD4 homologue (20% identity) was detected.…”

Section: Homologies To Type IV Secretion Systemsmentioning

confidence: 88%

“…All resistance mechanisms that have been identified in pathogenic bacteria, including RNA methylases, ATP-binding cassette transporters, aminoglycoside phosphotransferases, and ␤-lactamases, already exist in the respective antibiotic producers. In Streptomyces coelicolor (http://www.sanger.ac.uk /Projects/S_coelicolor/), as well as in the glycopeptide producers of the genus Amycolatopsis, even the vancomycin resistance determinants vanH (D-Ala dehydrogenase), vanA (D-Ala-DLac ligase), and vanX (D,D-dipeptidase) are present in the very same gene organization as found in the enterococcal conjugative transposon Tn1549 (79).…”

Section: Conjugative Transfer Of Resistance Determinants From Antibiomentioning

confidence: 99%

“…Orf5 (pIP501) shows a score of 71.2 and E value of 3 ϫ 10 Ϫ13 as a member of the VirB4 family of intracellular trafficking and secretion proteins (COG3451). Orf20, encoded by Tn1549, a VanB-type conjugative transposon of the Tn916 family, has significant similarity to TrsE, the VirB4 homologue encoded by pGO1 (27% identity in 437 of a total of 800 amino acids [79]). …”

Section: Homologies To Type IV Secretion Systemsmentioning

confidence: 99%

“…This plasmid could be transferred to E. coli and S. lividans, hosts in which the pIP501-located antibiotic resistance genes and transfer functions were functional (118a). A particular concern for public health related to transfer of antibiotic resistance genes in pathogenic gram-positive bacteria is that of resistance to vancomycin such as that encoded by conjugative transposons (79), which are quite common in the gut bacteria Enterococcus. While enterococcial infections are generally not life-threatening, the genes for vancomycin resistance may be spread to bacteria like S. aureus, which may cause fatal diseases.…”

Section: Implications Of Intergeneric Gene Transfer By Gram-positive mentioning

confidence: 99%

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Conjugative Plasmid Transfer in Gram-Positive Bacteria

Grohmann

Muth

Espinosa

2003

Microbiol Mol Biol Rev

506

447

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Conjugative transfer of bacterial plasmids is the most efficient way of horizontal gene spread, and it is therefore considered one of the major reasons for the increase in the number of bacteria exhibiting multiple-antibiotic resistance. Thus, conjugation and spread of antibiotic resistance represents a severe problem in antibiotic treatment, especially of immunosuppressed patients and in intensive care units. While conjugation in gram-negative bacteria has been studied in great detail over the last decades, the transfer mechanisms of antibiotic resistance plasmids in gram-positive bacteria remained obscure. In the last few years, the entire nucleotide sequences of several large conjugative plasmids from gram-positive bacteria have been determined. Sequence analyses and data bank comparisons of their putative transfer (tra) regions have revealed significant similarities to tra regions of plasmids from gram-negative bacteria with regard to the respective DNA relaxases and their targets, the origins of transfer (oriT), and putative nucleoside triphosphatases NTP-ases with homologies to type IV secretion systems. In contrast, a single gene encoding a septal DNA translocator protein is involved in plasmid transfer between micelle-forming streptomycetes. Based on these clues, we propose the existence of two fundamentally different plasmid-mediated conjugative mechanisms in gram-positive microorganisms, namely, the mechanism taking place in unicellular gram-positive bacteria, which is functionally similar to that in gram-negative bacteria, and a second type that occurs in multicellular gram-positive bacteria, which seems to be characterized by double-stranded DNA transfer

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Section: Homologies To Type IV Secretion Systemsmentioning

confidence: 88%

Section: Conjugative Transfer Of Resistance Determinants From Antibiomentioning

confidence: 99%

Section: Homologies To Type IV Secretion Systemsmentioning

confidence: 99%

Section: Implications Of Intergeneric Gene Transfer By Gram-positive mentioning

confidence: 99%

See 2 more Smart Citations

Conjugative Plasmid Transfer in Gram-Positive Bacteria

Grohmann

Muth

Espinosa

2003

Microbiol Mol Biol Rev

506

447

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“…In Europe, VanA-type Enterococcus faecium is the predominant type isolated from animals, humans, and environmental sources. The VanA gene is encoded on the Tn1546 transposon or a Tn1546-like transposon (8) and the VanB gene is encoded on a conjugative transposon, Tn1549 (9), and may potentially be associated with the transfer of resistance to other organisms, including Staphylococcus aureus (10). VanC-type glycopeptide resistance is consti-tutive; therefore, transfer to other organisms is not of much concern (11).…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Vancomycin Resistant Enterococci in Arak Hospitals

Zadeh

Shojapour

Nazari

et al. 2015

Jundishapur J Microbiol

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Background: Enterococcal species have emerged as important pathogens in Iran as well as throughout the world. With the increased use of vancomycin, Vancomycin-Resistant Enterococci (VRE) has become an important nosocomial pathogen. Objectives: The aim of the present study was to determine the incidence and antimicrobial susceptibility pattern of VRE and also to determine the most important genes that cause resistance to vancomycin in clinical samples in Arak, Iran. Materials and Methods:In total, 200 enterococci samples were collected from clinical specimens of Arak hospitals. Enterococcal species were identified using standard biochemical tests. Antibiotic susceptibility was tested by the Clinical and Laboratory Standards Institute (CLSI) disk diffusion. Minimum Inhibitory Concentration (MICs) was determined by broth micro dilution. All of the VRE isolates were examined by PCR to detect the presence of VRE genes. Results: Disk diffusion agar showed that 96 strains (48%) were resistant to gentamicin, 89 (44.5%) to ciprofloxacin, 127 (63.5%) to erythromycin, 142 (71%) to tetracycline, 11 (5.5%) to teicoplanin, 32 (16%) to vancomycin, none to linezolid and 96 (48%) to co-trimoxazole. The MICs of the resistant isolates were as follows; 88 strains had MIC ≥ 32 μg/mL to vancomycin and 59 strains had MIC ≥ 32 μg/mL to teicoplanin. Molecular studies revealed that 59.09% of VRE contained VanA genes and 7.95% of VRE contained the VanB genes. None of the strains had vanC1 and vanC2/3 gene. Conclusions: According to the results of this study, rates of vancomycin-resistance in enterococci, in Iran like other parts of the world, is increasing. Therefore accurate methods are required for identifying strains that possess resistance genes because many cases of hospital infections are caused by these strains.

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Pathogenomics of Enterococcus faecalis

Manson

Gilmore

2006

Pathogenomics

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Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp. The GenBank accession number for the 33803 bp sequence of Tn1549 is AJ192329.

Cited by 154 publications

References 43 publications

Conjugative Plasmid Transfer in Gram-Positive Bacteria

Conjugative Plasmid Transfer in Gram-Positive Bacteria

Genotyping of Vancomycin Resistant Enterococci in Arak Hospitals

Pathogenomics of Enterococcus faecalis

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