Characterization of trehalose synthase from Corynebacterium nitrilophilus NRC

Asker, Mohsen S.; Ramadan, Mohamed Fawzy; El-Aal, Samir Khalf Abd; El-Kady, Ebtsam M.

doi:10.1007/s11274-008-9950-9

Cited by 12 publications

(6 citation statements)

References 16 publications

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“…Separation was achieved by pumping acetonitrile-water (75:25 v/v) through the column at 1.0 mL/min for 20 min. The trehalose content after reaction was calculated based on the HPLC standard curves obtained by the trehalose standard (Sigma Co., St. Louis, USA) (Asker et al 2009). The conversion rate was calculated by the ratio of the trehalose product to the amount of maltose substrate as described previously ).…”

Section: Hplc Analysis Of Tres Activitymentioning

confidence: 99%

Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 for the scale-up production of trehalose from maltose

et al. 2014

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Trehalose synthase (TreS) is considered to be a potential biocatalyst for trehalose production. We aimed to scale-up produce the TreS protein in Escherichia coli and further investigate the bioconversion capacity of TreS. The treS gene from Pseudomonas putida KT2440 was amplified and expressed in E. coli BL21 (DE3). The recombinant TreS showed a molecular mass of 67 kDa. Activity analysis suggested that TreS had optimal activity at a temperature of 55 °C, a pH of 7.4, with a substrate concentration of 30%. High-pressure liquid chromatography results indicated that this enzyme had the ability to catalyze 59% maltose into trehalose, with about 5.1% glucose as by-product. Purification analysis showed that trehalose crystals with a purity of 98% were obtained by cooling trehalose solution. The TreS from P. putida KT2440 might be a candidate for trehalose production. Further study is needed to improve the trehalose conversion rate.

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Section: Hplc Analysis Of Tres Activitymentioning

confidence: 99%

Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 for the scale-up production of trehalose from maltose

et al. 2014

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“…A similar two‐step process for the production of trehalose from Sulfolobus shibatae with trehalosyl‐dextrin forming enzyme and trehalose‐forming enzyme has also been reported 10. Furthermore, direct conversion of maltose into trehalose with trehalose synthase, an enzyme capable of transforming α‐1,4 linkage into α‐ 1,1 linkage, has been proposed 11–23. Such process is particularly promising for the production of trehalose from maltose on industrial scales, because only one enzymatic step is involved.…”

Section: Introductionmentioning

confidence: 99%

Chemically Stable Perovskites as Cathode Materials for Solid Oxide Fuel Cells: La‐Doped Ba_0.5Sr_0.5Co_0.8Fe_0.2O_3−δ

Kim¹,

Choi²,

Jun³

et al. 2014

ChemSusChem

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Ba0.5Sr0.5Co0.8Fe0.2O(3-δ) (BSCF) has won tremendous attention as a cathode material for intermediate-temperature solid-oxide fuel cells (IT-SOFC) on the basis of its fast oxygen-ion transport properties. Nevertheless, wide application of BSCF is impeded by its phase instabilities at intermediate temperature. Here we report on a chemically stable SOFC cathode material, La0.5Ba0.25Sr0.25Co0.8Fe0.2O(3-δ) (LBSCF), prepared by strategic approaches using the Goldschmidt tolerance factor. The tolerance factors of LBSCF and BSCF indicate that the structure of the former has a smaller deformation of cubic symmetry than that of the latter. The electrical property and electrochemical performance of LBSCF are improved compared with those of BSCF. LBSCF also shows excellent chemical stability under air, a CO2-containg atmosphere, and low oxygen partial pressure while BSCF decomposed under the same conditions. Together with this excellent stability, LBSCF shows a power density of 0.81 W cm(-2) after 100 h, whereas 25 % degradation for BSCF is observed after 100 h.

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“…Trehalose is stable under hot and acidic conditions and has affinity for lipids in biological membranes and proteins. It is not only used in science, medicine and cosmetics (Asker et al 2009) but also can improve the tolerance of many organisms to adverse conditions. Trehalose is an ideal stress protectant.…”

Section: Introductionmentioning

confidence: 99%

Trehalose protects wine yeast against oxidation under thermal stress

Wang

et al. 2009

World J Microbiol Biotechnol

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Accumulation of trehalose in yeasts has been suggested to be an important mechanism of tolerance against adverse stress conditions, particularly in thermal stress. However, under thermal stress, it is not clear if the mechanism of protection is related to its antioxidant role. In this study, a newly isolated wine yeast Saccharomyces cerevisia was used to examine the protective effect of trehalose against oxidation during thermal stress treatment. Cells were treated either with a mild heat treatment at 37°C (which leads to trehalose accumulation) or with a 50 mM trehalose solution and then exposed to a high temperature of 53°C. According to our results, mild heat treatment at 37°C and trehalose addition which promote accumulation of trehalose significantly increased cell survival upon exposure to thermal stress at 53°C which seems to be correlated with decrease in reactive oxygen species levels and lipid peroxidation. Trehalose could protect yeast from oxidative injuries under thermal stress.

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Characterization of trehalose synthase from Corynebacterium nitrilophilus NRC

Cited by 12 publications

References 16 publications

Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 for the scale-up production of trehalose from maltose

Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 for the scale-up production of trehalose from maltose

Chemically Stable Perovskites as Cathode Materials for Solid Oxide Fuel Cells: La‐Doped Ba_0.5Sr_0.5Co_0.8Fe_0.2O_3−δ

Trehalose protects wine yeast against oxidation under thermal stress

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Characterization of trehalose synthase from Corynebacterium nitrilophilus NRC

Cited by 12 publications

References 16 publications

Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 for the scale-up production of trehalose from maltose

Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 for the scale-up production of trehalose from maltose

Chemically Stable Perovskites as Cathode Materials for Solid Oxide Fuel Cells: La‐Doped Ba0.5Sr0.5Co0.8Fe0.2O3−δ

Trehalose protects wine yeast against oxidation under thermal stress

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Chemically Stable Perovskites as Cathode Materials for Solid Oxide Fuel Cells: La‐Doped Ba_0.5Sr_0.5Co_0.8Fe_0.2O_3−δ