Characterization of two abundant satellite DNAs from the mealworm Tenebrio obscurus

Plohl, Miroslav; Ugarković, Đurđica

doi:10.1007/bf00173418

Cited by 22 publications

(13 citation statements)

References 36 publications

(35 reference statements)

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“…Likewise, mutational and spreading processes appear to contribute differently to the evolution of highly homologous satellite DNAs in two taxonomically related tenebrionid species. In contrast, they act in a comparable manner on two divergent satellite sequences found in one of these insects (Plohl and Ugarkovic 1994b).…”

Section: Introductionmentioning

confidence: 98%

Characterization of Interrelated Sequence Motifs in Four Satellite DNAs and Their Distribution in the Genome of the Mollusc Donax trunculus

Plohl

Cornudella

1997

J Mol Evol

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A family of four satellite DNAs has been characterized in the genome of the bivalve mollusc, Donax trunculus. All share HindIII sites, a similar monomer length of about 160 base pairs (bp), and the related oligonucleotide motifs GGTCA and GGGTTA, repeated six to 15 times within the repetitive units. The motif GGTCA is common to all members of the satellite family. It is present in three of them in both orientations, interspersed within nonrepetitive DNA sequences. The hexanucleotide GGGTTA appears to be the main building element of one of the satellites forming a prominent subrepeat structure in conjunction with the 5-bp motif. The former has been also found in perfect tandem repeats in a junction region adjacent to the proper satellite sequence. Southern analysis has revealed that (GGGTTA)n and/or related sequences are abundant and widely distributed in the D. trunculus genome. The distribution observed is consistent with the concurrence of the scattering of short sequence motifs throughout the genome and the spread of longer DNA segments, with concomitant formation of satellite monomer repeats. Both kinds of dispersion may have contributed to the observed complex arrangement of the HindIII satellite DNA family in Donax.

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Section: Introductionmentioning

confidence: 98%

Characterization of Interrelated Sequence Motifs in Four Satellite DNAs and Their Distribution in the Genome of the Mollusc Donax trunculus

Plohl

Cornudella

1997

J Mol Evol

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“…It is proposed that these structures perform a coding function in the binding of specific proteins and in this way influence condensation of heterochromatin (Vogt 1992). Three different satellites from tenebrionid species T. molitor, Tenebrio obscurus, and P. ratzeburgii have the same monomer length of 142 bp and form tertiary structure of very similar geometry in the form of a lefthanded superhelix (Plohl et al 1990;Ugarković et al 1992;Plohl and Ugarković 1994b). It is suggested that conservation of such structural characteristics despite sequence divergence points to their importance for heterochromatin formation (Plohl and Ugarković 1994b).…”

Section: Introductionmentioning

confidence: 99%

Evolution of Tribolium madens (Insecta, Coleoptera) Satellite DNA Through DNA Inversion and Insertion

1996

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Two different satellite DNAs from tenebrionid species Tribolium madens (Insecta, Coleoptera) have been detected, cloned, and sequenced. Satellite I comprises 30% of the genome; it has a monomer size of 225 bp and a high A + T content of 74%. Satellite II, with a monomer size of 711 bp and A + T content of 70%, is less abundant, making 4% of the total DNA. Sequence variability of the monomers relative to consensus sequence is 4.1% and 1.2% for satellite I and II, respectively. Both satellites are localized in the heterochromatic regions of all chromosomes. A search for internal motifs showed that both satellites contain a related subsequences, about 100 bp long. The creation of satellite I monomer is explained by duplication of the basic subunit, followed by subsequent divergence by single point mutations, deletions, and gene conversion. Inversion of the subsequence in addition to its duplication has occurred in satellite II. The result of this inversion is possible formation of a long, stable dyad structure. The 408-bp sequence, inserted within satellite II monomer, shares no similarity with a basic subunit. Frequent direct repeats found within the inserted sequence point to its evolution by duplication of shorter motifs. It is proposed that both satellites have been derived from a common ancestral sequence whose duplication played a major role in the formation of satellite I monomer, while insertion of a new sequence together with inversion of an ancestral one induced the occurrence of satellite II.

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“…The digitalization and densitometric measurement of the gels were performed with the aid of the Sun View program (Pharmacia). The satellite DNA percentage was estimated using the densitometric percentage of bands with respect to the whole DNA (Plohl & Ugarkovic, 1994).…”

Section: Measuring Dna Contentmentioning

confidence: 99%

Test of the correlation between body size and DNA content in Pimelia (Coleoptera: Tenebrionidae) from the Canary Islands

Palmer¹,

Petitpierre²,

Pons³

2003

Eur. J. Entomol.

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Abstract. Comparative analyses of interspecific data in evolutionary biology usually require specific methods to remove the effects of phylogenetic inertia. When phylogenetic inertia is not considered, the Canarian Pimelia species show a positive, and almost sig nificant (Prob. = 0.066) correlation between nuclear genome size and body size. However, after controlling for phylogenetic inertia there was a negative and significant correlation (Prob. = 0.007 to 0.017, depending on the DNA fraction considered). Such a change in the relationship after controlling for phylogenetic inertia is rarely reported. Moreover, the relationship usually reported is positive and thought be a consequence of species having a similar number of cells at the same stage of development. The aim of the present study is to report a case of a negative correlation, but not to explain the causal mechanism involved in genome size variations or pro pose a formal hypothesis on the specific links between DNA content and body size. However, a common explanation of the change in the relationship, i.e., positive to negative, is suggested. Moreover, the data available on the highly repetitive, non-coding satellite DNA allows us to analyse the specific pattern exhibited by this fraction.

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Characterization of two abundant satellite DNAs from the mealworm Tenebrio obscurus

Cited by 22 publications

References 36 publications

Characterization of Interrelated Sequence Motifs in Four Satellite DNAs and Their Distribution in the Genome of the Mollusc Donax trunculus

Characterization of Interrelated Sequence Motifs in Four Satellite DNAs and Their Distribution in the Genome of the Mollusc Donax trunculus

Evolution of Tribolium madens (Insecta, Coleoptera) Satellite DNA Through DNA Inversion and Insertion

Test of the correlation between body size and DNA content in Pimelia (Coleoptera: Tenebrionidae) from the Canary Islands

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