Characterization of two BHV-4 strains isolated in the Czech Republic

Abstract

This study describes the isolation of bovine herpesvirus 4 (BHV-4) from the respiratory tract of animals suffering from respiratory disease. DNA of new isolates, CH and Ni, was cleaved with BamHI, EcoRI and HindIII in restriction enzyme analysis and the fragments were identified by co-migration with the restriction profile of the reference strain Movar 33/63 cleaved with the appropriate endonuclease. Typical profiles with polyrepetitive DNA (prDNA) fragments were detected. In order to localize the size variation within the obtained digestion fragments, Southern blot hybridization was performed. Differences between the isolates CH, Ni were localized in both the prDNAs and the unique central part of the genome and were restricted to fragment size variation.

Common, Emerging, Vector-Borne and Infrequent Abortogenic Virus Infections of Cattle

“…It was first isolated in 1963 in Hungary from calves with respiratory and ocular lesions (Bartha et al, 1966). Later, many BHV‐4 isolates were recovered from all over the world; Belgium (Van Opdenbosch et al., 1988), Egypt (House et al., 1990), North Ireland (Graham et al., 2005), Japan (Yoshima et al., 2005), Serbia (Nikolin et al., 2007) and Czech Republic (Fichtelova and Kovarcik, 2010).…”