CHARACTERIZATION OF TWO HELA SUBLINES: TCRC-1 PRODUCES REGAN ISOENZYME AND TCRC-2, NON-REGAN ISOENZYME

Ultracytochemical localization of alkaline phosphatase isozymes in human cancer cell lines in vitro.

“…Production of alkaline phosphatase has been reported in several cultured human cancer cell lines (7,16,21,23,25). The isoenzymatic properties have also been characterized biochemically and immunologically in these cell lines.…”

“…In addition, production of the Kasahara isozyme might be enhanced by increased cell density (26). Suitable cloning of the heterogeneous cells may, however, provide subclones which are monophenotypic for a certain isozyme (13,23). If the cloned cell lines still exhibit two or more isozymes, proper culture conditions such as prednisolone treatment may provide a monophenotypic state.…”

“…Recently, the production of several cancer-associated alkaline phosphatase isozymes in human cancer cell lines in vitro has been reported (7,16,21,23,25). However, their cytochemical localization, especially from the isoenzymatic viewpoint, is still not clearly understood.…”

Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Degradation by Alkaline Phosphatase

“…HeLa cells are known for their high expression of PLAP (22,24,25). Furthermore, the enzymatic characteristics observed in HeLa cells in our study indicated that PLAP is the most prominent isoform in these cells.…”

Cellular localization of alkaline phosphatase isozymes in three human cancer cell lines: Methodological considerations based on electron cytochemical studies.

“…In addition, some human cancer cell lines expressing these isozymes have been reported, and have been characterized as monophenotypic to the term placental isozyme and its variant (1, 6, 14, 19, 20-22, 29, 34) or to the non-placental form (1,20,21,34,36,40,41).…”