Characterization of two molecular weight classes of cadmium binding proteins from the mussel, mytilus edulis (l.)

Frazier, John M.; George, Stephen S.; Overnell, Julian; Coombs, Thomas L.; Kagi, Jhr

doi:10.1016/0742-8413(85)90052-0

Cited by 34 publications

(26 citation statements)

References 14 publications

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“…Our results on MT components isolated by the conventional liquid chromatography techniques from the digestive gland of Cd-exposed mussels (M. galloprovincialis) are generally in agreement with the literature data previously reported for M. edulis (George et al 1979;Carpenè et al 1983;Frazier et al 1985;Mackay et al 1993). The authors report the existence of two molecular-mass classes which are separable by the conventional gelfiltration (Sephadex G-75) chromatography.…”

Section: Discussionsupporting

confidence: 80%

“…They were designated as MT-10 (monomer) and , following the terminology proposed by Mackay et al (1993). Further purification on a DEAE-Sephadex A-25 column indicates a great complexity of each molecular mass class which were resolved into several distinctively charged MT forms, similarly as previously demonstrated in M. edulis species (George et al 1979;Frazier et al 1985). The functional significance of that large number of multiple forms has still not been fully explained.…”

Section: Discussionmentioning

confidence: 91%

“…The existence of a possibly different type of MT dimer in M. edulis, presumably independent of S-S bridging, was first suggested by Frazier et al (1985), and was later confirmed by amino acid sequential analysis (Mackay et al 1993) on the basis of which the unique structure was proposed by forming dimers through intermolecular metal-bridging between two sulphur atoms (designed as S-M-S) with an additional Cd ion inserted. Consequently, the authors proposed an increasing metal complexing capacity and a possible distinction in mobility between such bridging Cd and of the Cd ions incorporated into cluster structure.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, in the American oyster, Crassostrea virginica, the monomeric acetylated form of MT (molecular mass 10,000) was predominant in naturally-occurring organisms and in those experimentally exposed to the low Cd level (Roesijadi 1994). In Cd-exposed M. edulis (George et al 1979;Roesijadi and Hall 1981;Köhler and Riisgard 1982;Frazier et al 1985;Bebianno and Langston 1991;Mackay et al 1993), the prevailing part of the total Cd-MT occurred in dimeric form. The proposed concept on differential Cd-mediated expression of specific MT forms was supported by evidence at the gene-expression level (Baršyte et al 1999;Lemoine et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…By comparison, in the Mediterranean species of the same genus, Mytilus galloprovincialis, a considerably smaller number of MT charge-distinct forms has been identified in selected organs and tissues (Carpenè et al 1983;Viarengo et al 1984;Pavičić et al 1991). In general, two molecular mass classes of MT, monomeric (MT-10) and dimeric , were determined in soft tissues of both species experimentally exposed to elevated levels of cadmium or mercury (George et al 1979;Frazier et al 1985;Roesijadi 1986). Further fractionation by conventional ion-exchange (DEAE) chromatography, or by reverse phase HPLC, reveals the high complexity of each mass class, particularly in M. edulis, being resolved in several charge-distinct components, putative isoforms.…”

Section: Introductionmentioning

confidence: 99%

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Multiple forms of metallothionein from the digestive gland of naturally occurring and cadmium-exposed mussels, Mytilus galloprovincialis

Ivanković

Pavičić

Kozar

et al. 2002

Helgoland Marine Research

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Polymorphism of metallothioneins in the digestive gland of naturally occurring (control) and experimentally Cd-exposed mussels Mytilus galloprovincialis (200 µg Cd l -1 ; 14 days) was studied by applying the conventional methods of Sephadex column liquid chromatography , and by an electrochemical method (DPASV) for determination of Cd, Zn and Cu concentrations in chromatographic fractions. In both control and Cd-exposed mussels, two distinct molecular mass components of the metallothioneins, monomeric (MT-10) and dimeric (MT-20), were resolved by Sephadex G-75 gel filtration chromatography. In control mussels, the MT-10 component was predominantly expressed as containing markedly higher constitutive levels of Zn (100×) and Cu (10×) than of Cd. Each of these two molecular mass components was further resolved into seven metal-rich peaks by anion-exchange chromatography. In Cd-exposed mussels the larger proportion of Cd was bound to the MT-20 than to the MT-10 component, suggesting that the dimeric component may be considered as a primarily inducible metallothionein. The elution positions of metal-binding maxima of Cd-exposed and control mussels on the respective DEAE chromatographic profiles were comparable. A great similarity in elution positions of Cd maxima between the composite and single-specimen samples was also observed. Our study confirms a high multiplicity of MT forms in mussels from the Mytilus genus not only under the laboratory high-level metal exposure conditions, but also at a natural seawater metal exposure level. The ecotoxicological significance of dimeric and monomeric MT forms, as well as their possible application in the biomonitoring of seawater for trace metals, has been considered.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Multiple forms of metallothionein from the digestive gland of naturally occurring and cadmium-exposed mussels, Mytilus galloprovincialis

Ivanković

Pavičić

Kozar

et al. 2002

Helgoland Marine Research

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Mercury(II) Binding to Metallothionein in Mytilus edulis revealed by High Energy‐Resolution XANES Spectroscopy

Manceau

Bustamante

Haouz

et al. 2018

Chemistry A European J

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Of all divalent metals, mercury (Hg II ) has the highest affinity for metallothioneins. Hg II is considered to be enclosed in the α and β domains as tetrahedral α‐type Hg 4 Cys 11‐12 and β‐type Hg 3 Cys 9 clusters similar to Cd II and Zn II . However, neither the four‐fold coordination of Hg nor the existence of Hg–Hg atomic pairs have ever been demonstrated, and the Hg II partitioning among the two protein domains is unknown. Using high energy‐resolution XANES spectroscopy, MP2 geometry optimization, and biochemical analysis, evidence for the coexistence of two‐coordinate Hg‐thiolate complex and four‐coordinate Hg‐thiolate cluster with a metacinnabar‐type (β‐HgS) structure in the α domain of separate metallothionein molecules from blue mussel under in vivo exposure is provided. The findings suggest that the CXXC claw setting of thiolate donors, which only exists in the α domain, acts as a nucleation center for the polynuclear complex and that the five CXC motifs from this domain serve as the cluster‐forming motifs. Oligomerization is driven by metallophilic Hg⋅⋅⋅Hg interactions. Our results provide clues as to why Hg has higher affinity for the α than the β domain. More generally, this work provides a foundation for understanding how metallothioneins mediate mercury detoxification in the cell under in vivo conditions.

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Bioaccumulation and metallothionein response in the asiatic clam (Corbicula fluminea) after experimental exposure to cadmium and inorganic mercury

Baudrimont

Metivaud

Maury-Brachet

et al. 1997

Enviro Toxic and Chemistry

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Abstract-The involvement of metallothioneins (MTs) in cadmium (Cd) and inorganic mercury (Hg [II]) bioaccumulation by the freshwater bivalve Corbicula fluminea was experimentally investigated after 0, 15, 30, and 45 d of exposure from the water column source. Three levels of contamination were studied for each metal: 0, 5, and 35 g Cd/L and 0, 1.45, and 5 g Hg/L, with two replicates per condition. Forty eight experimental units (EUs) were conducted simultaneously. The mollusks were fed twice a week by additions of phytoplanktonic algae. Quantification of MTs was done by Hg-saturation assay, using cold Hg(II). A partial purification of these proteins was conducted by gel-filtration chromatography, followed by Cd determinations in the different eluted fractions. Results at the whole organism (soft tissues) and organ or tissue group (gills, mantle, foot, visceral mass) levels show high metal concentrations, with a fourfold greater accumulation of inorganic Hg than Cd after 30 d exposure at the same concentration of 5 g/L. Gills and visceral mass were the principal storage compartments. A significant increase in MT concentrations was revealed in these two organs after exposure to Cd: ratios between the MT concentrations in contaminated and control mollusks were 2.4 and 2.8, respectively, for 5 and 35 g Cd/L. Cd burdens in the cytosol and in Յ18-kDa protein fractions, similar to purified mammal MTs, correspond to 30 and 14% of the total Cd accumulated in the whole organisms. No significant increase in MT biosynthesis was observed after exposure to inorganic Hg, despite the high metal concentrations in the organs.

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Characterization of two molecular weight classes of cadmium binding proteins from the mussel, mytilus edulis (l.)

Cited by 34 publications

References 14 publications

Multiple forms of metallothionein from the digestive gland of naturally occurring and cadmium-exposed mussels, Mytilus galloprovincialis

Multiple forms of metallothionein from the digestive gland of naturally occurring and cadmium-exposed mussels, Mytilus galloprovincialis

Mercury(II) Binding to Metallothionein in Mytilus edulis revealed by High Energy‐Resolution XANES Spectroscopy

Bioaccumulation and metallothionein response in the asiatic clam (Corbicula fluminea) after experimental exposure to cadmium and inorganic mercury

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