Characterization of two monoclonal antibodies directed against the 67 kDa high affinity laminin receptor and application for the study of breast carcinoma progression

Martignone, Stefania; Pellegrini, Rita; Villa, E.; Tandon, NN; Mastroianni, Antonio; Tagliabue, Elda; Ménard, Sylvie; Colnaghi, Maria I.

doi:10.1007/bf00133466

Cited by 53 publications

(41 citation statements)

References 29 publications

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“…MAb antiprogesterone receptor (1A6) was purchased from DBA (Segrate, MI, Italy). MAb anti-67Kd laminin receptor (MLuC5) has been described previously (Martignone et al, 1992).…”

Section: Antibodiesmentioning

confidence: 99%

Immunological and pathobiological roles of fibulin-1 in breast cancer

et al. 2003

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Fibulin-1 (Fbln-1) is an immunogenic breast cancerrelated glycoprotein identified by serological analysis of cDNA expression library (SEREX) strategy. Here, we show that dendritic cells from two breast cancer patients elicited a CD4 þ -mediated T-cell response to Fbln-1 presentation. In both patients, an antibody response to Fbln-1 was also found. By contrast, a Fbln-1-seronegative patient and a weakly seropositive patient demonstrated no such T-cell response. Analysis of human breast cancers for Fbln-1 RNA and protein expression revealed the presence of Fbln-1C and -1D variants. Fbln-1 was detected in the cytoplasm and at the cell surface of different human breast carcinoma cell lines. Immunohistochemical analysis of 528 archival primary breast carcinomas showed the expression of Fbln-1 in 35% of the cases. When the immunohistochemical findings were compared against pathobiological information associated with each specimen, an inverse relationship between Fbln-1 and cathepsin D expression was observed (P ¼ 0.04). Furthermore, even though long-term survival was similar between Fbln-1-positive and -negative cases, the survival of Fbln-1-positive cases improved when a lymphoid infiltrate was present at the tumour site. Taken together, our findings of an Fbln-1-specific immunity and the improved survival associated with Fbln-1 expression in the presence of lymphoid infiltration point to a role of Fbln-1 in tumour immunosurveillance.

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“…MAb antiprogesterone receptor (1A6) was purchased from DBA (Segrate, MI, Italy). MAb anti-67Kd laminin receptor (MLuC5) has been described previously (Martignone et al, 1992).…”

Section: Antibodiesmentioning

confidence: 99%

Immunological and pathobiological roles of fibulin-1 in breast cancer

et al. 2003

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“…MLuC5 antibody was previously demonstrated to block p67-mediated adhesion, thereby attenuating laminin binding. 24 Blockade of laminin binding by MLuC5 antibody was incomplete, seemingly due to HPC expression of additional laminin receptors, such as VLA-6. 14,25 Therefore, to test the effect of blocking p67-mediated binding on BM homing, BM or MPB CD34 ϩ cells were incubated with anti-p67Ab and injected into lethally irradiated NOD/SCID␤2m Ϫ/Ϫ recipients.…”

Section: Resultsmentioning

confidence: 99%

The p67 laminin receptor identifies human erythroid progenitor and precursor cells and is functionally important for their bone marrow lodgment

Bönig

Chang

Nakamoto

et al. 2006

Blood

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The laminins are a group of extracellular matrix proteins with constitutive expression in all tissues, including bone marrow stroma. A functional role for the nonintegrin laminin receptor p67 has been described for cancer metastasis and lymphocyte trafficking. Expression of p67 was also reported for other subsets of mature leukocytes and for malignant hematopoietic or nonhematopoietic cells. We explored p67 expression on normal hematopoietic progenitor cells (HPCs) and its putative role in bone marrow retention of transplanted HPCs. We found p67 expression on a subset of primary human CD34 ؉ cells coexpressing erythroid markers. Of importance, p67 recognizes early erythroid progenitors, since sorted p67 ؉ cells were significantly enriched for burst-forming units-erythroid (BFU-Es) and depleted of colony-forming unitsgranulocyte/macrophage (CFU-GMs).Blockade of p67 binding of donor cells, using antifunctional antibody, reduced bone marrow homing of BFU-Es. These studies identify p67 as a novel phenotypic marker for erythroid HPCs of functional importance for lineage-specific homing/retention among adult transplanted HPCs. IntroductionHoming of mature immunoregulatory cells to sites of inflammation follows a well-characterized sequence of rolling, firm adhesion, and transmigration. Although some of the same major players in this cascade are also involved in hematopoietic progenitor cell (HPC) homing to bone marrow (BM), differences in the hierarchy of molecular pathway usage exist, likely because of different tissue microenvironments and receptor repertoires. 1,2 ␣4/␤1-integrin/ VCAM-1 and CXCR4/SDF-1 are dominant pathways of hematopoietic-stromal cell interactions, important for retention of transplanted HPCs in BM. [3][4][5] In addition, an array of disparate dominant and ancillary molecules, 1,2 which work within a complex, interactive network, is involved in BM homing. 6,7 Within BM, lineagecommitted HPCs and stem cells are likely partitioned in different functional/spatial "niches," 8,9 to encounter an environment accommodating their specific functional requirements. How these niches are defined and the specific molecular interactions between seeded HPCs and their supportive microenvironmental niches are incompletely understood.Laminins are expressed in BM, and cognate receptors are expressed on HPCs, as demonstrated by HPC adhesion to laminin. [10][11][12] Instrumental roles of the nonintegrin laminin receptor p67 were described in metastasis, embryo implantation, and lymphocyte trafficking. 13,14 p67-mediated laminin binding was also reported for subsets of normal and malignant hematopoietic cells. [15][16][17][18] Expression of p67 on HPCs and its role in hematopoiesis were not previously addressed. Our present study shows that p67 recognizes erythroid progenitors/precursors, and that its inhibition blocks BM homing of BFU-Es. Study designAnimals and conditioning regimen NOD/SCID␤2microglobulin Ϫ/Ϫ mice (Jackson Laboratories, Bar Harbor, ME), housed and bred in the Helicobacter-free section of the Specifi...

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“…MGR1 and MGR2 are directed, respectively, against the c-erbB-1 and the c-erbB-2 oncogene products Tagliabue et al, 1991). MluC5 is directed against the 67 kDa laminin receptor (Martignone et al, 1992). For integrin expression, the MAR4 and MAR6 MAbs directed respectively against the PI and a6 subunits Bottini et al, 1993) Milan, Italy.…”

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confidence: 99%