Characterization of two novel ADP ribosylation factors from the shrimp Marsupenaeus japonicus

Ma, Jingyun; Zhang, Mingchang; Ruan, Lingwei; Shi, Hong; Xu, Xun

doi:10.1016/j.fsi.2010.08.003

Cited by 12 publications

(2 citation statements)

References 34 publications

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“…ADP ribosylation factors (Arfs), which are small ubiquitously expressed GTPases have been characterized in M. rosenbergii and M. japonicus. Arfs expression was up-regulated in both species after WSSV challenge [82, 83] [84]. The expression of Rab GTPases, Ras-like GTPase superfamily members, are up-regulated in response to WSSV [85] and MrNV infection.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of Macrobrachium rosenbergii (giant fresh water prawn) post-larvae in response to M. rosenbergii nodavirus (MrNV) infection: de novo assembly and functional annotation

et al. 2019

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BackgroundMacrobrachium rosenbergii, is one of a major freshwater prawn species cultured in Southeast Asia. White tail disease (WTD), caused by Macrobrachium rosenbergii nodavirus (MrNV), is a serious problem in farm cultivation and is responsible for up to 100% mortality in the post larvae stage. Molecular data on how M. rosenbergii post-larvae launches an immune response to an infection with MrNV is not currently available. We therefore compared the whole transcriptomic sequence of M. rosenbergii post-larvae before and after MrNV infection.ResultsTranscriptome for M. rosenbergii post-larvae demonstrated high completeness (BUSCO Complete: 83.4%, fragmentation: 13%, missing:3.3%, duplication:16.2%; highest ExN50 value: 94%). The assembled transcriptome consists of 96,362 unigenes with N50 of 1308 bp. The assembled transcriptome was successfully annotated against the NCBI non-redundant arthropod database (33.75%), UniProt database (26.73%), Gene Ontology (GO) (18.98%), Evolutionary Genealogy of Genes: Non-supervised Orthologous Groups (EggNOG) (20.88%), and Kyoto Encyclopedia of Genes and Genome pathway (KEGG) (20.46%). GO annotations included immune system process, signaling, response to stimulus, and antioxidant activity. Differential abundance analysis using EdgeR showed 2413 significantly up-regulated genes and 3125 significantly down-regulated genes during the infection of MrNV.ConclusionsThis study reported a highly complete transcriptome from the post-larvae stage of giant river prawn, M. rosenbergii. Differential abundant transcripts during MrNV infection were identified and validated by qPCR, many of these differentially abundant transcripts as key players in antiviral immunity. These include known members of the innate immune response with the largest expression change occurring in the M. rosenbergii post-larvae after MrNV infection such as antiviral protein, C-type lectin, prophenol oxidase, caspase, ADP ribosylation factors, and dicer.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of Macrobrachium rosenbergii (giant fresh water prawn) post-larvae in response to M. rosenbergii nodavirus (MrNV) infection: de novo assembly and functional annotation

et al. 2019

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“…Among DEGs with functional annotation, 13.8% and 13% were related to “ Vibrio cholerae ” and “amoebiasis” KEGG pathways respectively. Vibrio infections are very common in marine and aquatic environments, especially in arthropods 49 , 50 . They have been previously reported as opportunistic agents acting during heat stress, leading in some cases to massive mortalities of invertebrates such as echinoderms, cnidarians, and poriferans 51 – 53 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic response to thermal and salinity stress in introduced and native sympatric Palaemon caridean shrimps

Marie

Smith

Green

et al. 2017

Sci Rep

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Organisms develop local adaptations to cope with spatially and temporally variable environments such as estuarine habitats, where abiotic parameters such as salinity and temperature fluctuate continuously. Studying the regulation of gene expression in a variable environment allows us to understand the underlying molecular mechanisms of these adaptations and the relative roles of the genetic and plastic response. The transcriptomes of the European native Palaemon longirostris (PL) and the introduced P. macrodactylus (PM) shrimps are described and compared after an experiment simulating summer conditions in the Guadalquivir Estuary, Spain. Specimens, collected in the Guadalquivir Estuary, were maintained at a temperature and salinity of 20 °C and 5 ppt for the control, and 30 °C and 15 ppt for the stress treatment. A large amount of differential gene expression was observed: 16,013 and 2,594 for PL and PM respectively. Functionally annotated unigenes revealed some differences, with PL seemingly having to face stronger physiological stress than PM. Thus, PM seems to have greater resistance than PL under conditions of high temperature and salinity. These results constitute a step forward in the understanding of the underlying molecular mechanisms of genetic adaptation of native invertebrates, and alien taxa that have successfully invaded estuaries in temperate regions around the world.

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Molecular Characterization of Differentially Expressed ADP Ribosylation Factor from WSSV Infected Black Tiger Shrimp Penaeus monodon

Shekhar

Gomathi

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Characterization of two novel ADP ribosylation factors from the shrimp Marsupenaeus japonicus

Cited by 12 publications

References 34 publications

Transcriptomic analysis of Macrobrachium rosenbergii (giant fresh water prawn) post-larvae in response to M. rosenbergii nodavirus (MrNV) infection: de novo assembly and functional annotation

Transcriptomic analysis of Macrobrachium rosenbergii (giant fresh water prawn) post-larvae in response to M. rosenbergii nodavirus (MrNV) infection: de novo assembly and functional annotation

Transcriptomic response to thermal and salinity stress in introduced and native sympatric Palaemon caridean shrimps

Molecular Characterization of Differentially Expressed ADP Ribosylation Factor from WSSV Infected Black Tiger Shrimp Penaeus monodon

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