Characterization of two novel lactic acid bacteria isolated from the intestine of rainbow trout (Oncorhynchus mykiss, Walbaum) in Slovakia

Fečkaninová, Adriána; Koščová, Jana; Mudroňová, Dagmar; Schusterová, Petra; Maruščáková, Ivana Cingeľová; Popelka, Peter

doi:10.1016/j.aquaculture.2019.03.026

Cited by 38 publications

(33 citation statements)

References 47 publications

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“…Therefore, the higher viability in vivo can be assumed as compared to results received in vitro[29].The resistance to bile salts differs considerably among lactobacilli strains. However the growth delay and reduced volume of bacterial biomass was observed in all strains of Lactobacillus cultured in media with the addition of bile salts[17,38]. In our experiment growth delay was also noted, though it was statistically significant only from the beginning of the exponential phase (after 4th h).…”

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confidence: 38%

Influence of Zinc Sulphate on the Probiotic Properties of Lactobacillus plantarum CCM 7102

2019

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The effects of zinc sulphate on selected properties of L. plantarum CCM 7102 were tested in vitro. The resistance of lactobacilli to higher concentrations of ZnSO4 (up to 5000 mg Zn2+.l−1) in growth media was strain-dependent. Further studies were carried out on the most resistant strain of L. plantarum CCM 7102. While the addition of low concentrations of zinc sulphate into the growth media (< 100 mg Zn2+.l−1) did not influence the properties of L. plantarum CCM 7102, the concentrations of 100—500 mg Zn2+.l−1 stimulated: the growth rate, production of lactic acid, adhesion to porcine enterocytes and the inhibition of pathogens E. coli O8:K88+ent+, S. enterica and S. Typhimurium. Conversely, however, high concentrations > 500 mg Zn2+.l−1 inhibited these properties. The addition of zinc (250 mg Zn2+.l−1) did not affect the resistance to antimicrobials, low pH, and the resistance to bile salt was affected only weakly. Zinc-resistant probiotic Lactobacillus strains are suitable for use in feedstuffs with a higher content of zinc designed for the prevention of post weaning diarrhoea in pigs.

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confidence: 38%

Influence of Zinc Sulphate on the Probiotic Properties of Lactobacillus plantarum CCM 7102

2019

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“…Another study has found that Lactobacillus species are affected by many inhibitors of cell wall synthesis such as Penicillin and Ampicillin 31 . reported that P. pentosaceus LPP32, LPM83 and B5 were Clindamycin resistant 33 reported that among the different lactobacilli tested, they found high prevalence of resistances to Tetracycline (68% resistant isolates), Lincomycin (64.5%), Enrofloxacin (60%) and Ampicillin (50%) 34 founded that among 29 Lactobacillus strains only S4 (L. reuteri), S5 (L. plantarum), S8a (L. rhamnosus) and S8b (L. acidophilus) displayed resistance to Ampicillin and Penicillin.…”

Section: Discussionmentioning

confidence: 99%

Study the Probiotic Properties of Pediococcus pentosaceus Isolated from Fish Ponds in Basra City, South of Iraq

Jaafar¹,

Al-Knany²,

Mahdi³

et al. 2019

J Pure Appl Microbiol

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Citation: Raghad S. Jaafar, Fadhil N. Al-knany, Bayan A. Mahdi and Asaad M.R. Al-Taee, Study the Probiotic Properties of Pediococcus pentosaceus AbstractOne of the most important problems to the fish in aquaculture is pathogenic bacteria. Therefore, is a serious necessity in aquaculture to upgrade microbial hegemony strategies. The present study focused on isolation, screening, biochemical and molecular level characterizations of potential probiotic bacteria from the fish pond in Basra city southern Iraq. Isolated bacteria were characterized based on their morphological and biochemical traits and were identified using automated instrument (Vitek II) and 16S rRNA gene sequencing. As a result, bacteria were identified as Pediococcus pentosaceus. In order to be used in aquaculture as probiotics, bacteria must have good probiotic properties such as the ability to live in the presence of bile salts and low pH. Hence, bacteria were incubated with different concentrations of bile salts and pH values for different periods of time. In addition to that, bacteria were subjected to additional tests, such as tolerance to survive in simulated human gastrointestinal tract conditions and antibiotic susceptibility. Results of all these tests indicated that Pediococcus pentosaceus has good probiotic properties. The pathogenic bacteria (Salmonella sp.) were identified using the automated instrument for bacterial identification (Vitek II). The antagonistic ability of P. pentosaceus toward the pathogenic bacteria (Salmonella sp.) was tested using the agar sawing method. The result indicated that P. pentosaceus has good antagonistic ability.

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“…Aquatic sources and/or aquaculture are increasingly used to produce aquatic food all over the world. Fish are mostly reared in two fish farming facilities with a capacity of 140.503 m 3 and in 485 fish pools covering an area of about 2000 Ha [1, 2]. In Slovakia, trout is the most popular food fish, and aquaculture can be classified into two groups: fish farm and lowland wild fish species [2].…”

Section: Introductionmentioning

confidence: 99%

“…In general, the microbiota in trout from fish farms is more studied. There is for instance new information regarding the lactic acid bacteria (LAB) in trout from a commercial fish farm [2, 3], but limited data are available regarding trout from wild sources. Different LAB have adapted to grow under widely different environmental conditions, and they are widespread in nature.…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study was to check for enterococcal strain benefits in fish such as lactic acid production or enterocin genes; we tested for properties of enterococci to contribute to basic microbiology but also to select a potential candidate for inhibiting undesirable bacterial agents in trout. Beneficial strains for use in aquaculture should be regarded as safe, not only for the aquatic hosts but also for their surrounding environment and for humans (consumers) [2, 5]. Finally, our intention was preliminary studying enterococci on virulence factor parameter, biofilm formation, and antibiotic resistance regarding the safety aspect.…”

Section: Introductionmentioning

confidence: 99%

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Enterococci Isolated from Trout in the Bukovec Water Reservoir and Čierny Váh River in Slovakia and Their Safety Aspect

Lauková

Kubašová

Bino

et al. 2019

BioMed Research International

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The aim of this study was to investigate enterococci as lactic acid bacteria and as part of Firmicutes phylum. We focused on the virulence factor, biofilm formation, and antibiotic resistance and also on lactic acid production and enterocin gene detection. Intestinal samples were taken from 50 healthy trout (3 Salmo trutta and 47 Salmo gairdneri) collected in April 2007, 2010, and 2015 from different locations at the Bukovec water reservoir and the Čierny Váh River in Slovakia. Twenty pure colonies were identified using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification system based on protein fingerprints, and then seven identified strains were also phenotyped. Based on the identification methods used, the identified enterococci (7) belong taxonomically to four different enterococcal species: Enterococcus durans, E. faecium, E. mundtii, and E. thailandicus. They were hemolysis, DNase, and gelatinase negative with acceptable enzymatic activity. They did not form biofilm and were mostly susceptible to antibiotics. All strains produced lactic acid amounting to 1.78 ± 0.33 mmol/l on average and possessed the gene for enterocin A production. This is the first study reporting more detailed properties of enterococci from trout in Slovakian wild water sources, and it produces new possibilities for studying microbiota in trout.

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Characterization of two novel lactic acid bacteria isolated from the intestine of rainbow trout (Oncorhynchus mykiss, Walbaum) in Slovakia

Cited by 38 publications

References 47 publications

Influence of Zinc Sulphate on the Probiotic Properties of Lactobacillus plantarum CCM 7102

Influence of Zinc Sulphate on the Probiotic Properties of Lactobacillus plantarum CCM 7102

Study the Probiotic Properties of Pediococcus pentosaceus Isolated from Fish Ponds in Basra City, South of Iraq

Enterococci Isolated from Trout in the Bukovec Water Reservoir and Čierny Váh River in Slovakia and Their Safety Aspect

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