Characterization of Two Site-Specific Human Cytosolic Thioredoxin Mutants (Pro-34 to Ala and Val)

Characterization of Human Cytosolic Thioredoxin Mutants with Increasing Side Chain Volumes at Residue-33

“…Pro-34 was site-specifically mutated to Ala or Val. 4 These mutations did not affect the activity of Trx significantly, whereas the substrate availability by Trx reductase was affected. A mutation of Pro-34 to a smaller amino acid, Ala, resulted in Trx being a less efficient substrate to its reductase, whereas a mutation of Pro-34 to an amino acid with similar size and flexibility, Val, made the mutant a more efficient substrate.…”