Characterization of typical and atypical enteroaggregative Escherichia coli in Kagoshima, Japan: biofilm formation and acid resistance

Tokuda, Koichi; Nishi, Junichiro; Imuta, Naoko; Fujiyama, Rika; Kamenosono, Akira; Manago, Kunihiro; Kawano, Yoshifumi

doi:10.1111/j.1348-0421.2010.00210.x

Cited by 26 publications

(25 citation statements)

References 62 publications

(82 reference statements)

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“…The frequency of cefotaxime-resistance is lower than that described in other reports (24,34,35). Reportedly, the frequency of antimicrobial resistance is higher in EAEC strains isolated from symptomatic cases than from asymptomatic cases (36).…”

Section: Discussioncontrasting

confidence: 46%

Phenotypic and Molecular Characterization of Enteroaggregative <i>Escherichia coli</i> Isolated in Kawasaki, Japan

Kubomura¹,

Misaki²,

Homma³

et al. 2017

Jpn J Infect Dis

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Section: Discussioncontrasting

confidence: 46%

Phenotypic and Molecular Characterization of Enteroaggregative <i>Escherichia coli</i> Isolated in Kawasaki, Japan

Kubomura¹,

Misaki²,

Homma³

et al. 2017

Jpn J Infect Dis

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“…Strains were screened for the following genes: the transcriptional regulator aggR (TOKUDA et al, 2010); cnf1 and cnf2 (BLANCO et al, 1996); the toxin genes sta, Stx1, Stx2 (multiplex PCR) (FRANCK et al, 1998); intimin (eae) (BLANCO et al, 2006); bundle-forming pili (bfpA) (GUNZBURG; TORNIEPORTH; RILEY, 1995); ipaH for EIEC (ARANDA et al, 2007); Stx2e, the most frequent pathovar identified, F41, stb, LT, 987P, F18, K88, and K99 (multiplex PCR) (MACÊDO et al, 2007). The PCR reaction conditions were performed according to each author, with no modifications.…”

Section: Detection Of Virulence Genes and Characterization Of Phylogementioning

confidence: 99%

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

et al. 2018

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Detecção de genes de virulência e grupos filogenéticos de amostrasEscherichia coli isoladas de cães no Brasil INTRODUCTIONEscherichia coli are a component of normal intestinal microbiota in humans and other animals. Phenotypic and genotypic characteristics allow the identification of E. coli pathogenic strains or pathovars (COURA et al., 2014). Dogs are colonized by E. coli during the first days of their life and some strains can cause enteric or extra-intestinal infections (BEUTIN, 1999 (BEUTIN, 1999). Other pathovars are associated with diarrhea in other animals, namely enteroaggregative E. coli (EAEC) and enteroinvasive E. coli (EIEC) (MAINIL, 2013). Because of the close contact with humans, dogs could increase the risk of transmitting potentially zoonotic microorganisms, such as EPEC and STEC (BEUTIN, 1999).In addition to the pathotype and pathovar classification, E. coli strains can be assigned to one of the seven phylogenetic groups, including A, B1, B2, C, D, E, and F (CLERMONT et al., 2013). E. coli genomic structure has shown that the strains belonging to different phylogroups are associated with the disease state and source of isolation (CLERMONT et al., 2013). The extraintestinal pathogenic E. coli (ExPEC) strains are clustered mostly in groups B2 and D and intestinal pathogenic E. coli are mostly in phylogroups A, B1, and E (ESCOBAR- PÁRAMO et al., 2004; CLERMONT et al., 2011). Although phylogenetic characterization is an important tool to improve the understanding of E. coli populations and the relationship between strains and disease (CLERMONT et al., 2011;COURA et al., 2015), few studies have determined phylogenetic groups of E. coli isolated from dogs worldwide, and of those that did, the most frequently used method was a triplex PCR method developed by CLERMONT in 2000. This method is only capable of determining phylogroups A, B1, B2, and D (HARADA et al., 2012;SALVARANI et al., 2012;SCHMIDT et al., 2015) instead of the seven phylogroups that were detected in this study. In Brazil, only two studies identified E. coli pathovars associated with diarrhea in dogs (ALMEIDA et al., 2012;PUÑO-SARMIENTO et al., 2013), but none determined the phylogenetic groups of E. coli. Therefore, this study aimed to detect the virulence genes, pathovars, and phylogenetic groups of E. coli strains isolated from the feces of dogs with and without diarrhea. MATERIALS AND METHODS Stool samplesStool samples were collected from 154 dogs, of which 92 were diarrheic and 62 were without diarrhea. Samples from diarrheic dogs were obtained directly from the rectum, at the Veterinary Hospital of Universidade Federal de Minas Gerais (Belo Horizonte city), upon admission. Collections were performed from dogs that were undergoing a consultation for the occurrence of diarrhea. With owner's consent, fecal samples from non-diarrheic animals were obtained from the dogs of students attending the Universidade Federal de Minas Gerais. For non-diarrheic dogs, fecal samples were either taken directly from the rectum or after spontaneous ...

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“…E. coli strains that produce the CTX-M type of ESBLs are increasingly being isolated from adults and children with UTIs and bacteremia; however, CTX-M ESBLs have rarely been found among diarrheagenic E. coli (DEC) strains. We previously reported that 36.8% of the aggR gene-positive EAEC strains isolated from diarrheal children in Kagoshima, Japan, were cefotaxime resistant (22), suggesting ESBL production. Subsequently, some CTX-M-producing EAEC strains were reported worldwide (23)(24)(25)(26), including an EAEC-Shiga toxin-producing E. coli (STEC) hybrid clone of O104:H4 that caused a large outbreak in Germany in 2011 (27).…”

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confidence: 99%

Phylogenetic Analysis of Enteroaggregative Escherichia coli (EAEC) Isolates from Japan Reveals Emergence of CTX-M-14-Producing EAEC O25:H4 Clones Related to Sequence Type 131

et al. 2016

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f Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea. The aggR gene is widely used as a marker for typical EAEC. The heterogeneity of EAEC is well known; however, there are few reports on the phylogenetic relationships of EAEC. Recently, CTX-M extended-spectrum ␤-lactamase (ESBL)-producing EAEC strains have been reported worldwide. To characterize EAEC strains in Japan, we investigated the population structure of EAEC. A total of 167 aggR-positive strains isolated from stool specimens from diarrheal patients in Kagoshima (139 strains) and Osaka (28 strains), Japan, between 1992 and 2010 were examined for the prevalence of EAEC virulence markers, the bla CTX-M gene, and the capacity to form biofilms. Multilocus sequence typing was also conducted. EAEC strains were widely distributed across four major E. coli phylogroups. Strains of O111: H21/clonal group 40 (CG40) (30 strains), O126:H27/CG200 (13 strains), and O86a:H27/CG3570 (11 strains) in phylogroup B1 are the historical EAEC clones in Japan, and they exhibited strong biofilm formation. Twenty-nine strains of EAEC O25:H4/ CG131 were identified in phylogroup B2, 79% of which produced CTX-M-14. This clone has emerged since 2003. The clone harbored plasmid-encoded EAEC virulence genes but not chromosomal virulence genes and had lower biofilm-forming capacity than historical EAEC strains. This clone most likely emerged from a pandemic uropathogenic O25:H4/sequence type 131 clone by acquiring an EAEC virulence plasmid from canonical EAEC. Surveillance of the horizontal transfer of both virulence and ESBL genes among E. coli strains is important for preventing a worldwide increase in antimicrobial drug resistance. Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea in children and adults, in both developing (1) and industrialized (2, 3) countries. Several outbreaks of gastroenteritis linked to EAEC have been reported (4, 5). EAEC is also the second most common cause of travelers' diarrhea (6). EAEC is defined by its characteristic aggregative adherence (AA) to HEp-2 cells in culture (7). Although detection of the AA phenotype in the HEp-2 cell adherence assay is still the gold standard for EAEC identification, the transcriptional regulator AggR, which controls the expression of multiple EAEC virulence genes, is also used as a typical EAEC marker for PCR detection (8).The pathogenesis of EAEC infection involves the adherence of the bacterium to the intestinal mucosa, followed by the secretion of one or more enterotoxins and/or cytotoxins that induce mucosal inflammation (9). EAEC adherence to the mucosa is characterized by the formation of a thick biofilm (10), which may promote persistent infection. The majority of EAEC strains carry a large (ϳ100-kb) plasmid, which encodes most putative EAEC virulence factors, such as AggR, aggregative adherence fimbriae (AAF), the dispersin protein Aap (11), its transporter Aat (12), the plasmid-encoded toxin Pet (13), enteroaggregative heat-stable toxin 1 (EAST-1) (14), and the...

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Characterization of typical and atypical enteroaggregative Escherichia coli in Kagoshima, Japan: biofilm formation and acid resistance

Cited by 26 publications

References 62 publications

Phenotypic and Molecular Characterization of Enteroaggregative <i>Escherichia coli</i> Isolated in Kawasaki, Japan

Phenotypic and Molecular Characterization of Enteroaggregative <i>Escherichia coli</i> Isolated in Kawasaki, Japan

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

Phylogenetic Analysis of Enteroaggregative Escherichia coli (EAEC) Isolates from Japan Reveals Emergence of CTX-M-14-Producing EAEC O25:H4 Clones Related to Sequence Type 131

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