Characterization of ultrasonic vocalizations of Fragile X mice

Belagodu, Amogh P.; Johnson, Aaron M.; Galvez, Roberto

doi:10.1016/j.bbr.2016.04.016

Cited by 26 publications

(23 citation statements)

References 47 publications

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“…A previous study that used a different mating paradigm to elicit calls from the males also found that Fmr1 KO mice emitted the same number of calls compared to the WT mice. In contrast to our findings they did not detect any differences in the average duration of USVs (Belagodu et al, 2016). One other adult FXS USV study reported a significantly decreased amount of calls in Fmr1 KO mice (Rotschafer et al, 2012).…”

Section: Discussioncontrasting

confidence: 99%

“…The number of USVs may not be a key determinant in detecting communication deficits in FXS and other autism mouse models. Our results support recent findings suggesting call types and acoustic properties to be more representative of the atypical communication patterns observed in the syndrome (Belagodu et al, 2016; Holy & Guo, 2005; Scattoni et al, 2008). …”

Section: Discussionsupporting

confidence: 92%

“…This makes it difficult to determine whether analyzed USVs consisted of solely male vocalization patterns and that the presence of a female did not confound results. In a separate study, investigators used a different courtship paradigm to induce USVs and found no difference in the number of USVs emitted by adult male Fmr1 KO mice, but found an increase in “u” syllables, while WT littermates vocalized “h” syllables more often (Belagodu et al, 2016). While the functional importance of specific syllables based on frequency and complex patterns is currently unknown, they are organized into phrases that are thought to resemble repetitive speech patterns typically found in those with the syndrome.…”

Section: Introductionmentioning

confidence: 99%

“…The investigation of ultrasonic vocalization (USV) deficits has been previously explored in various mouse models of neurological diseases, both in early development from postnatal day (PD) 2 to 14 (Lai et al, 2014; Roy, Watkins, & Heck, 2012; Scattoni, Gandhy, Ricceri, & Crawley, 2008; Wohr, Roullet, Hung, Sheng, & Crawley, 2011; Wohr et al, 2013) and in adulthood ranging from PD60 to 120 (Belagodu, Johnson, & Galvez, 2016; Rotschafer, Trujillo, Dansie, Ethell, & Razak, 2012; Wohr, Roullet, Hung, et al, 2011). Mouse pups begin emitting ultrasonic vocalizations (USVs) shortly after birth and continue, although at a reduced rate, throughout adulthood in response to specific situations.…”

Section: Introductionmentioning

confidence: 99%

“…The few studies examining adult USVs in FXS focus mainly on courtship behavior (Belagodu et al, 2016; Rotschafer et al, 2012). Rotschafer et al (2012) found the number of USVs in adult male Fmr1 KO mice to be reduced compared to wild type (WT) controls in a mating paradigm (Rotschafer et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Spectral and temporal properties of calls reveal deficits in ultrasonic vocalizations of adult Fmr1 knockout mice

Hodges

Nolan

Reynolds

et al. 2017

Behavioural Brain Research

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The Fmr1 knockout (KO) mouse has commonly been used to investigate communication impairments, one of the key diagnostic symptoms observed in Fragile X syndrome (FXS) and Autism spectrum disorder (ASD). Many studies have found alterations in ultrasonic vocalizations (USVs) in neonatal Fmr1 KO mice, however, there is limited research investigating whether these deficits continue into adulthood. In the present study, we examine differences in female urine-induced ultrasonic vocalizations, scent marking behavior, odor discrimination, and open field activity in adult male Fmr1 KO and wildtype (WT) mice. Overall, we found extensive alterations between genotypes in both spectral and temporal properties of ultrasonic vocalizations. There was no difference in the average number of calls emitted by both genotypes, however, Fmr1 KO mice emitted calls of a higher frequency, decreased amplitude, and shorter duration than WT mice. Spectrographic analyses revealed statistically significant differences between genotypes in the types of calls emitted. Contrastingly, we found no differences in scent marking behavior, a form of social communication, or in odor discrimination and activity levels of the mice. The results corroborate previous studies emphasizing the importance of qualitative differences observed in vocalization behavior of Fmr1 KO mice, rather than quantitative measurements such as number of calls emitted. Overall, the study confirms the presence of abnormalities in vocalization behavior in adult Fmr1 KO mice that we believe are consistent with communication deficits seen in the syndrome.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Spectral and temporal properties of calls reveal deficits in ultrasonic vocalizations of adult Fmr1 knockout mice

Hodges

Nolan

Reynolds

et al. 2017

Behavioural Brain Research

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From synaptic dysfunction to atypical emotional processing in autism

Reis,

Monteiro

2024

FEBS Letters

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Autism spectrum disorder (ASD) is a complex neurodevelopmental condition mainly characterized by social impairments and repetitive behaviors. Among these core symptoms, a notable aspect of ASD is the presence of emotional complexities, including high rates of anxiety disorders. The inherent heterogeneity of ASD poses a unique challenge in understanding its etiological origins, yet the utilization of diverse animal models replicating ASD traits has enabled researchers to dissect the intricate relationship between autism and atypical emotional processing. In this review, we delve into the general findings about the neural circuits underpinning one of the most extensively researched and evolutionarily conserved emotional states: fear and anxiety. Additionally, we explore how distinct ASD animal models exhibit various anxiety phenotypes, making them a crucial tool for dissecting ASD's multifaceted nature. Overall, to a proper display of fear response, it is crucial to properly process and integrate sensorial and visceral cues to the fear‐induced stimuli. ASD individuals exhibit altered sensory processing, possibly contributing to the emergence of atypical phobias, a prevailing anxiety disorder manifested in this population. Moreover, these individuals display distinctive alterations in a pivotal fear and anxiety processing hub, the amygdala. By examining the neurobiological mechanisms underlying fear and anxiety regulation, we can gain insights into the factors contributing to the distinctive emotional profile observed in individuals with ASD. Such insights hold the potential to pave the way for more targeted interventions and therapies that address the emotional challenges faced by individuals within the autism spectrum.

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Communication and social interaction in the cannabinoid‐type 1 receptor null mouse: Implications for autism spectrum disorder

et al. 2021

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Clinical and preclinical findings have suggested a role of the endocannabinoid system (ECS) in the etiopathology of autism spectrum disorder (ASD). Previous mouse studies have investigated the role of ECS in several behavioral domains; however, none of them has performed an extensive assessment of social and communication behaviors, that is, the main core features of ASD. This study employed a mouse line lacking the primary endocannabinoid receptor (CB1r) and characterized ultrasonic communication and social interaction in CB1−/−, CB1+/−, and CB1+/+ males and females. Quantitative and qualitative alterations in ultrasonic vocalizations (USVs) were observed in CB1 null mice both during early development (i.e., between postnatal days 4 and 10), and at adulthood (i.e., at 3 months of age). Adult mutants also showed marked deficits in social interest in the three‐chamber test and social investigation in the direct social interaction test. These behavioral alterations were mostly observed in both sexes and appeared more marked in CB1−/− than CB1+/− mutant mice. Importantly, the adult USV alterations could not be attributed to differences in anxiety or sensorimotor abilities, as assessed by the elevated plus maze and auditory startle tests. Our findings demonstrate the role of CB1r in social communication and behavior, supporting the use of the CB1 full knockout mouse in preclinical research on these ASD‐relevant core domains. Lay Summary The endocannabinoid system (ECS) is important for brain development and neural function and is therefore likely to be involved in neurodevelopmental disorders such as Autism Spectrum Disorder (ASD). Here we investigated changes in social behavior and communication, which are core features of ASD, in male and female mice lacking the chief receptor of this system. Our results show that loss of this receptor results in several changes in social behavior and communication both during early development and in adulthood, thus supporting the role of the ECS in these ASD‐core behavioral domains.

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Characterization of ultrasonic vocalizations of Fragile X mice

Cited by 26 publications

References 47 publications

Spectral and temporal properties of calls reveal deficits in ultrasonic vocalizations of adult Fmr1 knockout mice

Spectral and temporal properties of calls reveal deficits in ultrasonic vocalizations of adult Fmr1 knockout mice

From synaptic dysfunction to atypical emotional processing in autism

Communication and social interaction in the cannabinoid‐type 1 receptor null mouse: Implications for autism spectrum disorder

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