Characterization of Ultrathin Poly(ethylene glycol) Monolayers on Silicon Substrates

Papra, Alexander; Gadegaard, Nikolaj; Larsen, Niels Bent

doi:10.1021/la000609d

Cited by 256 publications

(271 citation statements)

References 27 publications

Supporting

Mentioning

240

Contrasting

Unclassified

Order By: Relevance

“…43 No. 1) were cleaned using 1:1 HCl/methanol followed by a concentrated H 2 SO 4 soak for 2 h. The coverslips were then coated with a PEG-terminated silane following a modified protocol from Papra et al 46 Dry toluene was prepared by distillation over metallic sodium to remove any water or other contaminants. The alkylsilane (2-[methoxypol(ethyleneoxy)proply]trimethoxysilane, Gelest, Tullytown, PA) was added to the toluene to a final concentration of 0.1% by volume in an MBraun glovebox (MBraun, Stratham, NH).…”

Section: ■ Discussionmentioning

confidence: 99%

Two Cell Circuits of Oriented Adult Hippocampal Neurons on Self-Assembled Monolayers for Use in the Study of Neuronal Communication in a Defined System

Edwards

Stancescu

Molnár

et al. 2013

ACS Chem. Neurosci.

View full text Add to dashboard Cite

In this study, we demonstrate the directed formation of small circuits of electrically active, synaptically connected neurons derived from the hippocampus of adult rats through the use of engineered chemically modified culture surfaces that orient the polarity of the neuronal processes. Although synaptogenesis, synaptic communication, synaptic plasticity, and brain disease pathophysiology can be studied using brain slice or dissociated embryonic neuronal culture systems, the complex elements found in neuronal synapses makes specific studies difficult in these random cultures. The study of synaptic transmission in mature adult neurons and factors affecting synaptic transmission are generally studied in organotypic cultures, in brain slices, or in vivo. However, engineered neuronal networks would allow these studies to be performed instead on simple functional neuronal circuits derived from adult brain tissue. Photolithographic patterned self-assembled monolayers (SAMs) were used to create the two-cell "bidirectional polarity" circuit patterns. This pattern consisted of a cell permissive SAM, N-1[3-(trimethoxysilyl)propyl] diethylenetriamine (DETA), and was composed of two 25 μm somal adhesion sites connected with 5 μm lines acting as surface cues for guided axonal and dendritic regeneration. Surrounding the DETA pattern was a background of a non-cell-permissive poly(ethylene glycol) (PEG) SAM. Adult hippocampal neurons were first cultured on coverslips coated with DETA monolayers and were later passaged onto the PEG-DETA bidirectional polarity patterns in serum-free medium. These neurons followed surface cues, attaching and regenerating only along the DETA substrate to form small engineered neuronal circuits. These circuits were stable for more than 21 days in vitro (DIV), during which synaptic connectivity was evaluated using basic electrophysiological methods.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Two Cell Circuits of Oriented Adult Hippocampal Neurons on Self-Assembled Monolayers for Use in the Study of Neuronal Communication in a Defined System

Edwards

Stancescu

Molnár

et al. 2013

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…al. 1 for functionalizing the chips with PEG silane. PEG silane and concentrated HCl were mixed in toluene in a freshly cleaned glass beaker with 100 µL PEG and 40 µL HCl present in 50 mL of toluene.…”

Section: Functionalization Of Silicon With Peg Groupsmentioning

confidence: 99%

DNA-Based Programmed Assembly of Gold Nanoparticles on Lithographic Patterns with Extraordinary Specificity

2004

View full text Add to dashboard Cite

MATERIALS AND REAGENTS4" N-type silicon wafers were purchased from the Berkeley Microfabrication Laboratory. 2-[methoxy(polyethyleneoxy)propyl]trimethoxysilane (henceforth referred to as PEG silane) was purchased from Gelest, Inc. (Morrisville, PA.) 10 nm (nominal diameter) and 15 nm (nominal diameter) gold nanocrystals were purchased from Ted Pella, Inc. (Redding, CA.) Bis(p-sulfonatophenyl)phenylphosphine dihydrate dipotassium salt (henceforth referred to as Phosphine) was purchased from Cardinal Industries (Milwaukee, WI.) All oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA.) at the 1 micromole scale in a lyophilized (powder) form. The names and base sequences of the complementary DNA molecules are as follows (note that the first 20 bases from the 5' end function as spacers and do not hybridize):Substrate Oligo: 5'-SH-C6-TTTTTTTTTTTTTTTTTTTTAATATTGATAAGGAT NC Oligo: 5'-SH-C6-TTTTTTTTTTTTTTTTTTTTATCCTTATCAATATT

show abstract

“…11 Work has shown that it is possible to pattern the aminated alkylsilane DETA using deep-ultraviolet ͑DUV͒ photolithography. [12][13][14][15][16] Using DUV, SAMs were exposed to intense ultraviolet light from an ArF excimer laser ͑emission wavelength 193 nm͒. Upon exposure the SAM underwent a photochemical reaction that cleaved the carbon-nitrogen bonds within the molecule, thereby removing the cytophilic surface coating.…”

Section: Introductionmentioning

confidence: 99%

Direct patterning of coplanar polyethylene glycol alkylsilane monolayers by deep-ultraviolet photolithography as a general method for high fidelity, long-term cell patterning and culture

Wilson

Stancescu

Das

et al. 2011

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

View full text Add to dashboard Cite

This manuscript details a general method for patterning coplanar alkylsilane monolayers using deep-ultraviolet photolithography that has broad application for high fidelity patterning of cells of varying phenotype in long-term cultures. A polyethylene glycol monolayer was formed on a silica substrate and then patterned using 193 nm light from an ArF excimer laser. The regions of photoablation were then rederivatized with ͑3-trimethoxysilyl propyl͒ diethyltriamine ͑DETA͒, yielding high contrast cytophilic islands that promoted cell adhesion and growth. Rat hippocampal neurons, motoneurons, and myoblasts were then cultured in a defined, serum-free medium on the patterned surfaces for periods in excess of 40 days. This approach has been shown to be useful as a general method for the long-term culture of multiple cell types in highly defined spatial patterns and can be used for supporting complex cocultures for creating in vitro models for biological systems.

show abstract

Characterization of Ultrathin Poly(ethylene glycol) Monolayers on Silicon Substrates

Cited by 256 publications

References 27 publications

Two Cell Circuits of Oriented Adult Hippocampal Neurons on Self-Assembled Monolayers for Use in the Study of Neuronal Communication in a Defined System

Two Cell Circuits of Oriented Adult Hippocampal Neurons on Self-Assembled Monolayers for Use in the Study of Neuronal Communication in a Defined System

DNA-Based Programmed Assembly of Gold Nanoparticles on Lithographic Patterns with Extraordinary Specificity

Direct patterning of coplanar polyethylene glycol alkylsilane monolayers by deep-ultraviolet photolithography as a general method for high fidelity, long-term cell patterning and culture

Contact Info

Product

Resources

About