Characterization of upper airway ciliary beat by coupling isolated and collective cilia motion analysis

Abstract

ObjectiveCiliary dysfunctions may have deleterious consequences on mucociliary clearance. We propose a new approach based on coupling the isolated ciliary beat pattern and the global efficiency of ciliary beat on human ciliated cells. MethodsCiliated cells issued from nasal brushing (controls and primary ciliary dyskinesia patients) were recorded by high-speed video-microscopy (350 frames s -1 ). We have performed an original quantitative analysis of ciliary beat dynamics (CBD) by following cilium tips. It allows to describe different parameters including ciliary beat frequency also measured by Fast-Fourier-Transform and Video-Kymography. We have also developed the microbeads tracking method (MBT) to get an index of the global efficiency of ciliary beat. Here, micro-beads (4.5 µm) have been used as markers of the flow generated by beating cilia.

A new index for characterizing micro-bead motion in a flow induced by ciliary beating: Part I, experimental analysis

“…An original method of micro-bead tracking (MBT) is presented where micro-beads act as markers of the flow generated by the cilia [24]. A numerical model is then developed (presented in detail in a companion paper [25]) based on envelope modeling approach, allowing us to simulate the flow generated by the cilia as in the MBT experiment.…”

“…This present study aims at a deeper understanding of the relationship between ciliary beat pattern, measured by ciliary beating analysis, and the induced motion of the surrounding fluid in order to assess the global efficiency of ciliary beating. An original method of micro-bead tracking (MBT) is presented where micro-beads act as markers of the flow generated by the cilia [ 24 ]. A numerical model is then developed (presented in detail in a companion paper [ 25 ]) based on envelope modeling approach, allowing us to simulate the flow generated by the cilia as in the MBT experiment.…”