Characterization of Urine-Derived Stem Cells Obtained from Upper Urinary Tract for Use in Cell-Based Urological Tissue Engineering

Abstract

uUSC possess expansion and differentiation (urothelial and myogenic) capabilities, and can potentially be used as an alternative cell source in bladder tissue engineering for patients needing cystoplasty.

Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells

“…The results were in correlation with those of the literature (Zhang et al 2008;Bharadwaj et al 2011). USCs were positive for C73, CD105, and CD44 and negative for CD45 and CD31 (Fig.…”

“…The isolation procedure was performed as described in the literature (Zhang et al 2008;Bharadwaj et al 2011;Guan et al 2014). The cells were cultivated in keratinocyte serum-free medium (KSFM; Invitrogen,USA) and embryonic fibroblast medium in a 1:1 ratio, 10 % heat-inactivated fetal bovine serum, 1 % Pen-Strep antibiotics, and supplemented with 5 ng/ml EGF and 50 ng/ml bovine pituitary extract.…”

Silver nanoparticles promote osteogenic differentiation of human urine-derived stem cells at noncytotoxic concentrations

“…Four 250-300 mL mid stream urine samples from four healthy men aged 28-34 years were collected and directly transferred to the laboratory for isolation and culture, as reported previously, [2][3][4][5]7,8 with a slight modification. Briefly, urine specimens were centrifuged at 400× g for 10 minutes at room temperature.…”

“…Qin et al from urine and expanded in special culture, can differentiate into cell lineages such as urothelial cells, smooth muscle cells, endothelial cells, osteocytes, chondrocytes, and adipocytes on induction with appropriate media, [4][5][6][7][8] and have been reported recently to be ideal candidates for mesenchymal stem cells to replace marrow-derived stem cells and adipose-derived stem cells as seed cells for tissue engineering, due to their harvest from autologous urine using noninvasive, safe, low-cost, and easily reproducible methods. 3,7 Currently, USCs are being investigated as cell therapies in the treatment of renal insufficiency and urinary incontinence, and for tissue engineering in bladder and urethral tissue regeneration; 6 however, their use in tissue-engineering to repair bone defects is equally attractive.…”

“…USCs at passage 3 were characterized using flow cytometry analysis as described elsewhere. [3][4][5] Briefly, cells were trypsinized, washed in ice-cold phosphate-buffered saline containing 0.5% bovine serum albumin (wash buffer), and counted. Fluorochrome-conjugated antibodies, anti-CD34-fluorescein isothiocyanate (FITC), CD45-FITC, CD90-FITC, CD29-phycoerythrin, and CD73-phycoerythrin (BD Pharmingen, Franklin Lakes, NJ, USA), were added to 5 × 10 5 cells in 50 µL of phosphate-buffered saline containing 3% bovine serum albumin and incubated on ice for 30 minutes in the dark.…”