Characterization of variants in the promoter of EBV gene BZLF1 in normal donors, HIV-positive patients and in AIDS-related lymphomas

Martini, Maurizio; Capello, Daniela; Serraino, Diego; Navarra, Assunta; Pierconti, Francesco; Cenci, Tonia; Gaïdano, Gianluca; Larocca, Luigi Maria

doi:10.1016/j.jinf.2006.04.015

Cited by 21 publications

(28 citation statements)

References 32 publications

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“…1A). In contrast to the first report [Gutierrez et al, 2002], the occurrence of Zp À P as the only detectable variant in IM is consistent with the other two reports [Tong et al, 2003;Martini et al, 2007]. The Zp À V3 variant, identical to the EBV from AG876 [Dolan et al, 2006] and associated with EBV2, was described by Gutierrez et al [2002] exclusively in 48.1% of malignant samples.…”

Section: Resultssupporting

confidence: 87%

“…The Zp À V3 variant, identical to the EBV from AG876 [Dolan et al, 2006] and associated with EBV2, was described by Gutierrez et al [2002] exclusively in 48.1% of malignant samples. This observation was later confirmed by Tong et al [2003] (73.2%) and by Martini et al [2007] (50%). However, these more recent reports also described Zp À V3 in PBMC from healthy carriers and IM patients [Tong et al, 2003] (50%), [Martini et al, 2007] (27%).…”

Section: Resultsmentioning

confidence: 56%

“…This observation was later confirmed by Tong et al [2003] (73.2%) and by Martini et al [2007] (50%). However, these more recent reports also described Zp À V3 in PBMC from healthy carriers and IM patients [Tong et al, 2003] (50%), [Martini et al, 2007] (27%). Similarly, the present study demonstrates the presence of Zp À V3 variant in 6/13 (46.1%) lymphoma samples and in PBMC and throat swabs from 1/10 (10%) pediatric IM patient as the unique infective variant (Fig.…”

Section: Resultsmentioning

confidence: 56%

“…This Zp variant was later found by Tong et al [2003] in 46.6% of adult NPC from Asia and 52.8% of healthy adults. Martini et al [2007] also detected this variant in 49.2% of AIDS related lymphomas as well as in 73% of PBMC from healthy Italian donors. In the present pediatric series, Zp À P was detected in PBMC and throat swabs from 8/10 (80%) IM samples and in 5/13 (38.5%) tumor biopsies (Fig.…”

Section: Resultsmentioning

confidence: 76%

“…G)], is identical to that hold by AG876 cell line and finally Zp À V4 which holds the same three substitutions as Zp À V3 plus an additional one at position À196 (T ! C) [Gutierrez et al, 2002;Tong et al, 2003;Martini et al, 2007]. These variants of Zp appeared to be associated with the two major types of EBV, namely 1 and 2, which differ in the sequence of the genes encoding for nuclear antigens EBNA2, 3A, 3B, and 3C [Sample et al, 1990].…”

Section: Introductionmentioning

confidence: 99%

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Epstein–Barr virus BZLF1 gene promoter variants in pediatric patients with acute infectious mononucleosis: Its comparison with pediatric lymphomas

Lorenzetti

Gutiérrez²,

Altcheh

et al. 2009

Journal of Medical Virology

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Epstein-Barr virus genotypes can be distinguished by polymorphic variations in the genes encoding EBNA2, 3A, 3B, and 3C. The immediate early gene BZLF1 plays a key role in modulating the switch from latency to lytic replication and therefore enabling viral propagation. The aim of this study was to investigate and compare BZLF1 promoter sequence (Zp) variation in pediatric infectious mononucleosis (IM) and in pediatric EBV positive lymphoma biopsies. Zp was sequenced from peripheral blood mononuclear cells (PBMC) and throat swabs from 10 patients with IM at the time of diagnosis (D0) and during convalescence; and from 13 lymphoma biopsies. Zp - P and Zp - V3 variants were found in eight and one IM patients, as well as in five and six tumor biopsies, respectively. A correlation between viral genotype and Zp variant was found significant for Zp - V3 and EBV2 (P = 0.0002). One IM patient harbored two concomitant Zp variants. Regardless of anatomical compartment or stage of disease all IM patients displayed the same Zp variant along the course of the study. No new infections or adaptative selection of different variants was evidenced. A new Zp variant (Zp - V3 + 49) was described in two Hodgkin lymphomas, but not in IM. This is the first study to describe Zp variants compartmentalization in children with acute EBV infection and convalescence in a developing country; and comparing them with Zp variants in pediatric lymphomas from the same geographic area.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

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Epstein–Barr virus BZLF1 gene promoter variants in pediatric patients with acute infectious mononucleosis: Its comparison with pediatric lymphomas

Lorenzetti

Gutiérrez²,

Altcheh

et al. 2009

Journal of Medical Virology

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Epstein–Barr Virus Strain Variation

Farrell

2015

Current Topics in Microbiology and Immunology

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What is wild-type Epstein-Barr virus and are there genetic differences in EBV strains that contribute to some of the EBV-associated diseases? Recent progress in DNA sequencing has resulted in many new Epstein-Barr virus (EBV) genome sequences becoming available. EBV isolates worldwide can be grouped into type 1 and type 2, a classification based on the EBNA2 gene sequence. Type 1 transforms human B cells into lymphoblastoid cell lines much more efficiently than type 2 EBV and molecular mechanisms that may account for this difference in cell transformation are now becoming understood. Study of geographic variation of EBV strains independent of the type 1/type 2 classification and systematic investigation of the relationship between viral strains, infection and disease are now becoming possible. So we should consider more directly whether viral sequence variation might play a role in the incidence of some EBV-associated diseases.

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Epstein–Barr Virus: From the Detection of Sequence Polymorphisms to the Recognition of Viral Types

Feederle

Klinke

Kutikhin

et al. 2015

Current Topics in Microbiology and Immunology

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The Epstein-Barr virus is etiologically linked with the development of benign and malignant diseases, characterized by their diversity and a heterogeneous geographic distribution across the world. The virus possesses a 170-kb-large genome that encodes for multiple proteins and non-coding RNAs. Early on there have been numerous attempts to link particular diseases with particular EBV strains, or at least with viral genetic polymorphisms. This has given rise to a wealth of information whose value has been difficult to evaluate for at least four reasons. First, most studies have looked only at one particular gene and missed the global picture. Second, they usually have not studied sufficient numbers of diseased and control cases to reach robust statistical significance. Third, the functional significance of most polymorphisms has remained unclear, although there are exceptions such as the 30-bp deletion in LMP1. Fourth, different biological properties of the virus do not necessarily equate with a different pathogenicity. This was best illustrated by the type 1 and type 2 viruses that markedly differ in terms of their transformation abilities, yet do not seem to cause different diseases. Reciprocally, environmental and genetic factors in the host are likely to influence the outcome of infections with the same virus type. However, with recent developments in recombinant virus technology and in the availability of high throughput sequencing, the tide is now turning. The availability of 23 complete or nearly complete genomes has led to the recognition of viral subtypes, some of which possess nearly identical genotypes. Furthermore, there is growing evidence that some genetic polymorphisms among EBV strains markedly influence the biological and clinical behavior of the virus. Some virus strains are endowed with biological properties that explain crucial clinical features of patients with EBV-associated diseases. Although we now have a better overview of the genetic diversity within EBV genomes, it has also become clear that defining phenotypic traits evinced by cells infected by different viruses usually result from the combination of multiple polymorphisms that will be difficult to identify in their entirety. However, the steadily increasing number of sequenced EBV genomes and cloned EBV BACS from diseased and healthy patients will facilitate the identification of the key polymorphisms that condition the biological and clinical behavior of the viruses. This will allow the development of preventative and therapeutic approaches against highly pathogenic viral strains.

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Characterization of variants in the promoter of EBV gene BZLF1 in normal donors, HIV-positive patients and in AIDS-related lymphomas

Cited by 21 publications

References 32 publications

Epstein–Barr virus BZLF1 gene promoter variants in pediatric patients with acute infectious mononucleosis: Its comparison with pediatric lymphomas

Epstein–Barr virus BZLF1 gene promoter variants in pediatric patients with acute infectious mononucleosis: Its comparison with pediatric lymphomas

Epstein–Barr Virus Strain Variation

Epstein–Barr Virus: From the Detection of Sequence Polymorphisms to the Recognition of Viral Types

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