Characterization of Vascular Injury Responses to Stent Insertion in an Ex-vivo Arterial Perfusion Model

Kamat, Niharika; Nguyen-Ehrenreich, Kim Lien-Thi; Hsu, Steven; Ma, Alex P.; Sinn, Irene; Coleman, Leslie; Tai, Julie

doi:10.1016/j.jvir.2010.10.006

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…However, these studies with living pigs require adequate animal housing and operating equipment, are time-consuming (1–3 months), are expensive, and are ethically more and more difficult to implement [5]. Ex vivo models would be an inexpensive and quick approach to assess the impact of different stent design parameters on the vascular structure or response [22]. Ultimately, one would only examine the optimal stent/balloon in pigs.…”

Section: Introductionmentioning

confidence: 99%

Introduction of a New ex vivo Porcine Coronary Artery Model: Evaluation of the Direct Vascular Injury after Stent Implantation with and without Dogbone Effect

Obermaier¹,

Lehle²,

Schmid³

et al. 2022

Eur Surg Res

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Introduction: Neointimal hyperplasia after percutaneous coronary intervention remains a major determinant of in-stent restenosis (ISR). The extent of mechanical vessel injury correlates with ISR. A new ex vivo porcine stent model was introduced and evaluated comparing different stent designs. Methods: Coronary arteries were prepared from pig hearts from the slaughterhouse and used for ex vivo implantations of coronary stents. One basic stent design in two configurations (dogbone, DB; non-dogbone, NDB) was used. Vascular injury was determined according to a modified injury score (IS). Results: Standardized experimental conditions ensured comparable vessel dimensions and overstretch data. DB stents caused more severe IS compared to NDB stents. The mean IS and the IS at the distal end of all stents were significantly reduced for NDB stents (ISMean, DB, 1.16 ±0.12; NDB, 1.02 ±0.12; p=0.018; ISDist, DB, 1.39 ±0.28; NDB, 1.13 ±0.24; p=0.03). Discussion/Conclusion: The introduced ex-vivo model allowed the evaluation of different stent designs exclude unfavorable stent designs.

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Section: Introductionmentioning

confidence: 99%

Introduction of a New ex vivo Porcine Coronary Artery Model: Evaluation of the Direct Vascular Injury after Stent Implantation with and without Dogbone Effect

Obermaier¹,

Lehle²,

Schmid³

et al. 2022

Eur Surg Res

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“…They evaluated drug uptake and cellular proliferation in explanted human internal mammary artery. Characterization of stent-induced vascular injury response using an ex vivo arterial perfusion model has also been performed using harvested porcine carotid arteries ( 30 , 31 ). Harvested porcine arteries can maintain functionality up to 7 days under perfusion conditions ( 31 ).…”

Section: Introductionmentioning

confidence: 99%

The Development of an ex vivo Flow System to Assess Acute Arterial Drug Retention of Cardiovascular Intravascular Devices

Cooper

Cawthon

Goel

et al. 2021

Front. Med. Technol.

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Purpose: The goal of this study was to develop an ex vivo system capable of rapidly evaluating arterial drug levels in living, isolated porcine carotid arteries.Methods: A vascular bioreactor system was developed that housed a native porcine carotid artery under physiological flow conditions. The ex vivo bioreactor system was designed to quantify the acute drug transfer of catheter-based drug delivery devices into explanted carotid arteries. To evaluate our ex vivo system, a paclitaxel-coated balloon and a perfusion catheter device delivering liquid paclitaxel were utilized. At 1-h post-drug delivery, arteries were removed, and paclitaxel drug levels measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Parallel experiments were performed in a pig model to validate ex vivo measurements.Results: LC-MS/MS analysis demonstrated arterial paclitaxel levels of the drug-coated balloon-treated arteries to be 48.49 ± 24.09 ng/mg and the perfusion catheter-treated arteries to be 25.42 ± 9.74 ng/mg at 1 h in the ex vivo system. Similar results were measured in vivo, as arterial paclitaxel concentrations were measured at 59.23 ± 41.27 ng/mg for the drug-coated balloon-treated arteries and 23.43 ± 20.23 ng/mg for the perfusion catheter-treated arteries. Overall, no significant differences were observed between paclitaxel measurements of arteries treated ex vivo vs. in vivo.Conclusion: This system represents the first validated ex vivo pulsatile system to determine pharmacokinetics in a native blood vessel. This work provides proof-of-concept of a quick, inexpensive, preclinical tool to study acute drug tissue concentration kinetics of drug-releasing interventional vascular devices.

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“…This area of research has promoted interaction among scientific and industrial communities leading to innovation of new biomaterials in healthcare [ 1 – 5 ]. Various in vivo models have been developed to understand vascular biology and effect of therapeutic agents delivered by biomedical devices [ 6 – 8 ]; among these models, ex vivo tissue culture system has been described as an alternative model to in vivo animal and in vitro cell culture models to evaluate vascular responses in relation to biomechanical and biochemical factors [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Sensor-EnabledEx VivoBioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability

Mundargi

Venkataraman

Kumar

et al. 2015

BioMed Research International

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The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow. The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator “resorufin.” Our ex vivo bioreactor allows real time monitoring of tissue responses along with physiological conditions. These ex vivo parameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels. Histological evaluations with hematoxylin and eosin and Masson's trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements. Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices.

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Characterization of Vascular Injury Responses to Stent Insertion in an Ex-vivo Arterial Perfusion Model

Cited by 6 publications

References 16 publications

Introduction of a New ex vivo Porcine Coronary Artery Model: Evaluation of the Direct Vascular Injury after Stent Implantation with and without Dogbone Effect

Introduction of a New ex vivo Porcine Coronary Artery Model: Evaluation of the Direct Vascular Injury after Stent Implantation with and without Dogbone Effect

The Development of an ex vivo Flow System to Assess Acute Arterial Drug Retention of Cardiovascular Intravascular Devices

Novel Sensor-EnabledEx VivoBioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability

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