Characterization of vasculogenic potential of human adipose-derived endothelial cells in a three-dimensional vascularized skin substitute

Klar, Agnes S.; Güven, Sinan; Zimoch, Jakub; Zapiórkowska, Natalia A.; Biedermann, Thomas; Böttcher‐Haberzeth, Sophie; Meuli‐Simmen, Claudia; Martín, Iván; Scherberich, Arnaud; Ernst, Rolf; Meuli, Martin

doi:10.1007/s00383-015-3808-7

Cited by 77 publications

(87 citation statements)

References 40 publications

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“…showed that the endothelial part of SVF exhibited the ability to form microvascular structures in vitro and support the accelerated blood perfusion in skin substitutes in vivo . 9 This study confirmed their previous experiments and also the study conducted by Atalay et al . in 2014.…”

Section: Discussionsupporting

confidence: 92%

“…Many studies in the aesthetic field for skin rejuvenation 6 confirm that ADSCs increase the dermal collagen synthesis and even vascularity via production of many cytokines and growth factors. 7-9 It has been shown that ADSCs secrete growth factors that have an effect on fibroblast and keratinocyte proliferation. 10-12 Some researchers reported that the ADSCs could be differentiated into epithelial lineage and make their effect on the aged or damaged skin.…”

Section: Introductionmentioning

confidence: 99%

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Rejuvenation of facial skin and improvement in the dermal architecture by transplantation of autologous stromal vascular fraction: a clinical study

Amirkhani¹,

Shoae-Hassani²,

Soleimani³

et al. 2016

Bioimpacts

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Introduction: The rejuvenation characteristics of fat tissue grafting has been established for many years. Recently it has been shown that stromal vascular fraction (SVF) of fat tissue contributes to its rejuvenation properties. As the SVF is a minimal processed cell population (based on FDA guidance), therefore it is a suitable cell therapy for skin rejuvenation. This clinical trial was aimed to evaluate the ultrastructural improvement of aging skin in the facial nasolabial region after transplantation of autologous SVF. Methods: Our study was conducted in 16 patients aged between 38 and 56 years old that were interested in face lifting at first. All of the cases underwent the lipoaspiration procedure from the abdomen for sampling of fat tissue. Quickly, the SVF was harvested from 100 mL of harvested fat tissue and then transplanted at dose of 2.0×107 nucleated cells in each nasolabial fold. The changes in the skin were evaluated using Visioface scanner, skin-scanner DUB, Visioline, and Cutometer with multi probe adopter. Results: By administration of autologous SVF, the elasticity and density of skin were improved significantly. There were no changes in the epidermis density in scanner results, but we noticed a significant increase in the dermis density and also its thickness with enrichment in the vascular bed of the hypodermis. The score of Visioface scanner showed slight changes in wrinkle scores. The endothelial cells and mesenchymal progenitors from the SVF were found to chang the architecture of the skin slightly, but there was not obvious phenotypic changes in the nasolabial grooves. Conclusion: The current clinical trial showed the modification of dermis region and its microvascular bed, but no changes in the density of the epidermis. Our data represent the rejuvenation process of facial skin by improving the dermal architecture.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Rejuvenation of facial skin and improvement in the dermal architecture by transplantation of autologous stromal vascular fraction: a clinical study

Amirkhani¹,

Shoae-Hassani²,

Soleimani³

et al. 2016

Bioimpacts

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“…Several studies have been conducted to assess the efficacy of fat grafting on facial rejuvenation [1, 15-17], with the results suggesting that such rejuvenation effects result from the improvement of deep facial structures. In contrast, others have suggested that ASCs, which are abundant in fat tissue, participate in soft tissue remodeling by promoting collagen synthesis, cellular matrix protein synthesis, and revascularization of the dermis via the production of many cytokines and multipotential proliferation [17-20]. …”

Section: Discussionmentioning

confidence: 99%

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

Liang

Lü

et al. 2018

Cell Physiol Biochem

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Background/Aims: The rejuvenation properties of nanofat grafting have been described in recent years. However, it is not clear whether the clinical efficacy of the procedure is attributable to stem cells or linked to other components of adipose tissue. In this study we isolated nanofat-derived stem cells (NFSCs) to observe their biological characteristics and evaluate the efficacy of precise intradermal injection of nanofat combined with platelet-rich fibrin (PRF) in patients undergoing facial rejuvenation treatment. Methods: Third-passage NFSCs were isolated and cultured using a mechanical emulsification method and their surface CD markers were analyzed by flow cytometry. The adipogenic and osteogenic nature and chondrogenic differentiation capacity of NFSCs were determined using Oil Red O staining, alizarin red staining, and Alcian blue staining, respectively. Paracrine function of NFSCs was evaluated by enzyme-linked immunosorbent assay (ELISA) at 1, 3, 7, 14, and 28 days after establishing the culture. Then, the effects of PRF on NFSC proliferation were assessed in vitro. Finally, we compared the outcome in 103 patients with facial skin aging who underwent both nanofat and intradermal PRF injection (treatment group) and 128 patients who underwent hyaluronic acid (HA) injection treatment (control group). Outcomes in the two groups were compared by assessing pictures taken at the same angle before and after treatment, postoperative recovery, incidence of local absorption and cysts, and skin quality before treatment, and at 1, 12, 24 months after treatment using the VISIA Skin Image Analyzer and a SOFT5.5 skin test instrument. Results: NFSCs expressed CD29, CD44, CD49d, CD73, CD90, and CD105, but did not express CD34, CD45, and CD106. NFSCs also differentiated into adipocytes, osteoblasts, and chondrocytes under appropriate induction conditions. NFSCs released large amounts of growth factors such as VEGF, bFGF, EGF, and others, and growth factor levels increased in a time-dependent manner. At the same time, PRF enhanced proliferation of NFSCs in vitro in a dose-dependent manner, and the growth curves under different concentrations of PRF all showed plateaus 6d after seeding. Facial skin texture was improved to a greater extent after combined injection of nanofat and PRF than after control injection of HA. The nanofat-PRF group had a higher satisfaction rate. Neither treatment caused any complications such as infection, anaphylaxis, or paresthesia during long-term follow-up. Conclusion: NFSCs demonstrate excellent multipotential differentiation and paracrine function, and PRF promotes proliferation of NFSCs during the early stage after seeding. Both nanofat-PRF and HA injection improve facial skin status without serious complications, but the former was associated with greater patient satisfaction, implying that nanofat-PRF injection is a safe, highly effective, and long-lasting method for skin rejuvenation.

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“…The enzymatic digestion of fat tissue allowed for the production and separation of tissue components via centrifugation, ultimately providing the ability to isolate buoyant adipocytes and dense cellular pellets, the latter of which would be described as stromal vascular fraction. Within this dense cellular pellet there are a multitude of cell types including endothelial cells, smooth muscle cells, fibroblasts, pericytes, perivascular cells, immune cells, adipose stem cells, and mesenchymal stem cells [84][85][86][87][88] .…”

Section: The Stromal Vascular Fractionmentioning

confidence: 99%

“…systems that can provide a stimulating microenvironment for embedded cells in order to mature into tissue units 88,214 . Most 3D systems utilize a mixture of cells with a scaffold or substrate that provides sites for cell adhesion and migration, as well as threedimensionality, more closely mimicking endogenous tissue histological layouts.…”

Section: Am Live Stain At Day 2 (B) and Day 6 (F) Ethidium Homodimermentioning

confidence: 99%

Tissue engineered micro and macrovasculature utilizing stromal vascular fraction.

Zakhari¹

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Characterization of vasculogenic potential of human adipose-derived endothelial cells in a three-dimensional vascularized skin substitute

Cited by 77 publications

References 40 publications

Rejuvenation of facial skin and improvement in the dermal architecture by transplantation of autologous stromal vascular fraction: a clinical study

Rejuvenation of facial skin and improvement in the dermal architecture by transplantation of autologous stromal vascular fraction: a clinical study

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

Tissue engineered micro and macrovasculature utilizing stromal vascular fraction.

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