Characterization of Vibrio parahaemolyticus and its specific phage from shrimp pond in Palk Strait, South East coast of India

Stalin, Nattan; Srinivasan, Pappu

doi:10.1016/j.biologicals.2016.08.003

Cited by 47 publications

(24 citation statements)

References 52 publications

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“…5 μL of 0.5% uranyl acetate was then applied to the grids, and excess solution was immediately removed, and grids were air-dried. Samples were viewed with the FEI Tecnai G2 S-Twin Transmission Electron at an operating voltage at 80 KV (Sangha et al, 2014; Kwiatek et al, 2015; Stalin and Srinivasan, 2016).…”

Section: Methodsmentioning

confidence: 99%

Bacteriophages inhibit biofilms formed by multi-drug resistant bacteria isolated from septic wounds

Rani

Lakshmi

Praasad

2019

Preprint

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Section: Methodsmentioning

confidence: 99%

Bacteriophages inhibit biofilms formed by multi-drug resistant bacteria isolated from septic wounds

Rani

Lakshmi

Praasad

2019

Preprint

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“…Our results suggested that phages can be found in virtually all places where their related hosts exist. Several other investigations reported the presence of Vibrio-specific lytic phages in environmental water (shrimp ponds, aquaculture system, sea water and aquatic product market) from different regions of the world (Alagappan et al, 2010;Luo et al, 2016;Stalin and Srinivasan, 2016;Katharios et al, 2017;Srinivasan and Ramasamy, 2017;Yang M. et al, 2019).…”

Section: Origin and Morphology Of Phages Ba3 And Ca8mentioning

confidence: 99%

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

Yang

Liang

Huang³

et al. 2020

Front. Microbiol.

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Accumulating evidence has indicated that the multiple drug resistant Vibrio parahaemolyticus may pose a serious threat to public health and economic concerns for humans globally. Here, two lytic bacteriophages, namely vB_VpS_BA3 and vB_VpS_CA8, were isolated from sewage collected in Guangzhou, China. Electron microscopy studies revealed both virions taxonomically belonged to the Siphoviridae family with icosahedral head and a long non-contractile tail. The double-stranded DNA genome of phage BA3 was composed of 58648 bp with a GC content of 46.30% while phage CA8 was 58480 bp with an average GC content of 46.42%. In total, 85 putative open reading frames (ORFs) were predicted in the phage BA3 genome while 84 were predicted in that of CA8. The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions. Furthermore, average nucleotide identity analysis, comparative genomic features and phylogenetic analysis revealed that BA3 and CA8 represented different isolates but novel members of the family, Siphoviridae. Regarding the host range of the 61 V. parahaemolyticus isolates, BA3 and CA8 had an infectivity of 8.2 and 36.1%, respectively. Furthermore, ∼100 plaque-forming units (pfu)/cell for phage BA3 and ∼180 pfu/cell for phage CA8 were determined to be the viral load under laboratory growth conditions. Accordingly, the phage-killing assay in vitro revealed that phage CA8 achieved approximately 3.65 log unit reductions. The present results indicate that CA8 is potentially applicable for biological control of multidrug resistant V. parahaemolyticus.

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“…This study also elucidates that the phage is able to hijack protein translation machinery of the host bacteria. In comparison to other phages that infect vibrios (Table S2) [46][47][48][49][50][51][52] , phage Seahorse has relatively big burst size with a shorter latency period than phages VP-1, VP-2, VP-3, VpKK5, and VhKM4 that replicate in V. parahaemolyticus 27,29,[53][54][55][56] . It is also tolerant to the wide range of pHs and temperatures compared to other vibriophages suggesting unique survivability in harsh natural environments.…”

Section: Discussionmentioning

confidence: 96%

A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery

Thammatinna

Egan

Htoo

et al. 2020

Sci Rep

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Since the emergence of deadly pathogens and multidrug-resistant bacteria at an alarmingly increased rate, bacteriophages have been developed as a controlling bioagent to prevent the spread of pathogenic bacteria. One of these pathogens, disease-causing Vibrio parahaemolyticus (Vp AHPND ) which induces acute hepatopancreatic necrosis, is considered one of the deadliest shrimp pathogens, and has recently become resistant to various classes of antibiotics. Here, we discovered a novel vibriophage that specifically targets the vibrio host, VP AHPND . The vibriophage, designated Seahorse, was classified in the family Siphoviridae because of its icosahedral capsid surrounded by head fibers and a non-contractile long tail. Phage Seahorse was able to infect the host in a broad range of pH and temperatures, and it had a relatively short latent period (nearly 30 minutes) in which it produced progeny at 72 particles per cell at the end of its lytic cycle. Upon phage infection, the host nucleoid condensed and became toroidal, similar to the bacterial DNA morphology seen during tetracycline treatment, suggesting that phage Seahorse hijacked host biosynthesis pathways through protein translation. As phage Seahorse genome encodes 48 open reading frames with many hypothetical proteins, this genome could be a potential untapped resource for the discovery of phage-derived therapeutic proteins.Vibrio is a genus of motile Gram-negative bacteria that possesses a curved-rod cell shape with a single flagellum. Vibrios are abundant and diverse bacteria that are typically found in marine habitats. The genus Vibrio consists of 14 recognized clades and at least 86 different species 1 . While some of them are not pathogenic, many can cause serious health effects in both human and aquatic life. Due to the continuously rising ocean temperature, the composition of vibrio in the ocean microbiome has been reported to be higher than usual 2-4 . This vibrio-rich environment might increase the incident of a vibrio outbreak in the near future posing risks to global health 2 .Vibrio parahaemolyticus, which is one of the disease-causing Vibrio species, is pathogenic to both humans and marine animals 5 . Consumption of raw seafoods contaminated with the bacteria can cause acute gastroenteritis 5,6 . This opportunistic bacterium is also able to infect through an open wound which can lead to sepsis and, in rare cases, subsequent death in immunocompromised patients 7,8 . Moreover, V. parahaemolyticus that has acquired a plasmid encoding the deadly binary toxins PirA vp /PirB vp is even more virulent 9 . The V. parahaemolyticus strain harboring the plasmid has been found to cause a newly emerging disease in shrimp, known as acute hepatopancreatic necrosis disease (AHPND) 9 . Moreover, the AHPND-causing plasmid is also found to be transferable among other vibrios, increasing the chance of the disease spreading regionally and globally 10 . Unsurprisingly, the spread of AHPND has been reported in many countries, including China, Vietnam, Malaysia, Thailand, Me...

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Characterization of Vibrio parahaemolyticus and its specific phage from shrimp pond in Palk Strait, South East coast of India

Cited by 47 publications

References 52 publications

Bacteriophages inhibit biofilms formed by multi-drug resistant bacteria isolated from septic wounds

Bacteriophages inhibit biofilms formed by multi-drug resistant bacteria isolated from septic wounds

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery

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