Characterization of Virulence Factors And Antibacterial Activity of Curcumin in Hypervirulent
            <i>Klebsiella Pneumoniae</i>

Karami-Zarandi, Morteza; Ghaleh, Hadi Esmaeili Gouvarchin; Ranjbar, Reza

doi:10.2217/fmb-2021-0222

Cited by 4 publications

(2 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study, tea polyphenols at sub-MICs inhibited biofilm formation and protease production in K. pneumoniae [ 64 ]. In another study, curcumin possessed appropriate antibiofilm and anti-capsule activities of hypervirulent K. pneumoniae [ 65 ]. Also, Vitamin C inhibits biofilm and capsule formation in hypervirulent carbapenem-resistant K. pneumoniae [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Crippling of Klebsiella pneumoniae virulence by metformin, N-acetylcysteine and secnidazole

M. Shafik,

Abbas,

Yousef

et al. 2023

BMC Microbiol

View full text Add to dashboard Cite

Introduction The emergence of multidrug-resistant Klebsiella pneumoniae in hospitals represents a serious threat to public health. Infections caused by Klebsiella pneumoniae are widespread in healthcare institutions, mainly pneumonia, bloodstream infections, and infections affecting neonates in intensive care units; so, it is necessary to combat this pathogen with new strategies. Targeting virulence factors necessary to induce host damage and disease is a new paradigm for antimicrobial therapy with several potential benefits that could lead to decreased resistance. Background The influence of metformin, N-acetylcysteine, and secnidazole on Klebsiella pneumoniae virulence factors production was tested. The production of Klebsiella pneumoniae virulence factors such as biofilm formation, urease, proteases, hemolysins, and tolerance to oxidative stress was evaluated phenotypically using sub-inhibitory concentration (1/8 MIC) of metformin, N-acetylcysteine, and secnidazole. For more confirmation, qRT-PCR was used to assess the relative expression level of rmpA, wcaG, fimH-1, mrkD, ureA, and khe genes regulating virulence factors production. Results Metformin, N-acetylcysteine, and secnidazole were all found to have a powerful inhibitory effect on the production of virulence factors phenotypically. Our results showed a significant reduction in the expression level of rmpA, wcaG, fimH-1, mrkD, ureA, and khe genes. Furthermore, the tested drugs were investigated in vivo to inform their ability to protect mice against Klebsiella pneumoniae pathogenesis. Conclusions Metformin, N-acetylcysteine, and secnidazole inhibited the virulence of Klebsiella pneumoniae. Besides combating resistant Klebsiella pneumoniae, the tested drugs could also serve as an adjuvant to traditional antibiotics.

show abstract

Section: Discussionmentioning

confidence: 99%

Crippling of Klebsiella pneumoniae virulence by metformin, N-acetylcysteine and secnidazole

M. Shafik,

Abbas,

Yousef

et al. 2023

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…Curcumin can improve anti-Klebsiella (hypervirulent K. pneumoniae) treatment according to the crucial role of biofilm, capsule and efflux systems in the pathogenesis. 12 Java turmeric (C. xanthorrhiza Roxb.) with curcumin also has antibacterial activity against K. pneumoniae, which causes pneumonia.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Red Ginger (Zingiber officinale var. rubrum) and Black Turmeric (Curcuma caesia) Extracts as Growth Inhibitors of Klebsiella pneumonia

2023

TJNPR

View full text Add to dashboard Cite

Secondary plant metabolites play important role as potent drug candidates against antibacterialresistant pathogens such as Klebsiella pneumoniae which commonly causes pneumonia. Red ginger (Zingiber officinale var. rubrum) and black turmeric (Curcuma caesia) from the Zingiberaceae family are the potential plants as antibacterial agents. This study aimed to determine the ability of antibacterial activity and the inhibition mechanism of red ginger, black ginger and mixed (red ginger and black turmeric) extracts on the growth of K. pneumoniae. The method used was in vitro testing using the dilution method. Results showed that red ginger, black ginger and mixed ethanol extracts could inhibit K. pneumoniae growth at concentrations of 125 µg/mL and 250µg/mL, respectively, with a marked decrease in absorbance values before and after incubation. Further observations on bacterial cell leakage showed that the higher concentration of mixed ethanol extract, red ginger and black turmeric, the higher the leakage of K. pneumoniae bacterial cells seen from the increase in absorbance values that could be captured by wavelengths of 260 nm and 280 nm, respectively. Based on scanning electron microscope (SEM), the quantity of K. pneumoniae after treating black turmeric, red ginger, and mixed ethanol extracts decreased, and the cell walls became wrinkled and destroyed. Hence, ethanol extract from red ginger and black turmeric can be recommended as an alternative natural antibacterial in inhibiting the growth of K. pneumoniae, which causes pneumonia infection.

show abstract

Secondary metabolites of plants and their possible role in the “age of superbugs”

Nesterovich,

Belykh,

Gorokhovets

et al. 2023

BIOMED KHIM

View full text Add to dashboard Cite

Bacterial infections are a serious cause of high morbidity and mortality worldwide. Over the past decades, the drug resistance of bacterial pathogens has been steadily increasing, while the rate of development of new effective antibacterial drugs remains consistently low. The plant kingdom is sometimes called a bottomless well for the search for new antimicrobial therapies. This is due to the fact that plants are easily accessible and cheap to process, while extracts and components of plant origin often demonstrate a high level of biological activity with minor side effects. The variety of compounds obtained from plant raw materials can provide a wide choice of various chemical structures for interaction with various targets inside bacterial cells, while the rapid development of modern biotechnological tools opens the way to the targeted production of bioactive components with desired properties. The objective of this review is to answer the question, whether antimicrobials of plant origin have a chance to play the role of a panacea in the fight against infectious diseases in the “post-antibiotic era”.

show abstract

Characterization of Virulence Factors And Antibacterial Activity of Curcumin in Hypervirulent Klebsiella Pneumoniae

Cited by 4 publications

References 53 publications

Crippling of Klebsiella pneumoniae virulence by metformin, N-acetylcysteine and secnidazole

Crippling of Klebsiella pneumoniae virulence by metformin, N-acetylcysteine and secnidazole

Antibacterial Activity of Red Ginger (Zingiber officinale var. rubrum) and Black Turmeric (Curcuma caesia) Extracts as Growth Inhibitors of Klebsiella pneumonia

Secondary metabolites of plants and their possible role in the “age of superbugs”

Contact Info

Product

Resources

About