Characterization of virus-like particles by atomic force microscopy in ambient conditions

Oropesa, Reinier; Ramos, Jorge R.; Falcón, Viviana; Felipe, Ariel Santana

doi:10.1088/2043-6262/4/2/025007

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…3 a shows the topography distribution over a large area of HIV-1 Gag VLPs on mica substrate. HIV-1 Gag VLP preparations present similar topography to those observed by Oropesa et al (14) and Faivre-Moskalenko et al (15). Moreover, it can highlight the large number of VLP-like structures in the sample.…”

Section: Advanced Characterization Of Hiv-1 Gag Vlps By Mf Afmsupporting

confidence: 80%

“…When the HIV-1 Gag VLP diameter is calculated in the mean height as performed by Faivre-Moskalenko et al (15), a diameter of~150 nm is obtained, which is in agreement with previous measurements performed with TEM. Nonetheless, the determination of the particle size for this sample is affected by the adsorption effect of VLPs onto the substrate, hindering the determination of the real particle size by AFM (14).…”

Section: Identification Of Hiv-1 Gag Vlp By Afmmentioning

confidence: 99%

“…AFM has already been used in virus and VLP characterization providing, to our knowledge, novel information about biological samples, such as morphology, structural data, and composition in ambient conditions (14)(15)(16). Interestingly, recent advances in multifrequency (MF) AFM modes offer a broad assessment of nanomechanical features of soft samples.…”

Section: Introductionmentioning

confidence: 99%

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Identification of HIV-1–Based Virus-like Particles by Multifrequency Atomic Force Microscopy

Gutiérrez-Granados

Cervera

Gòdia

et al. 2016

Biophysical Journal

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Virus-like particles (VLPs) have become a promising platform for vaccine production. VLPs are formed by structural viral proteins that inherently self-assemble when expressed in a host cell. They represent a highly immunogenic and safe vaccine platform, due to the absence of the viral genome and its high protein density. One of the most important parameters in vaccine production is the quality of the product. A related bottleneck in VLP-based products is the presence of cellular vesicles as a major contaminant in the preparations, which will require the set up of techniques allowing for specific discrimination of VLPs from host vesicular bodies. In this work novel, to our knowledge, multifrequency (MF) atomic force microscopy (AFM) has permitted full structural nanophysical characterization by its access to the virus capsid of the HIVbased VLPs. The assessment of these particles by advanced amplitude modulation-frequency modulation (AM-FM) viscoelastic mapping mode has enhanced the imaging resolution of their nanomechanical properties, opening a new window for the study of the biophysical attributes of VLPs. Finally, the identification and differentiation of HIV-based VLPs from cellular vesicles has been performed under ambient conditions, providing, to our knowledge, novel methodology for the monitoring and quality control of VLPs.

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Section: Advanced Characterization Of Hiv-1 Gag Vlps By Mf Afmsupporting

confidence: 80%

Section: Identification Of Hiv-1 Gag Vlp By Afmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of HIV-1–Based Virus-like Particles by Multifrequency Atomic Force Microscopy

Gutiérrez-Granados

Cervera

Gòdia

et al. 2016

Biophysical Journal

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“…AFM is a powerful technique for measuring particle size and size distribution even in ambient conditions. In this approach, single particles can be imaged without a high cost for sample preparation [124,125]. The measurement of VLP particle size is accomplished through asymmetric flow field-flow fractionation coupled with multiple-angle light scattering (AF4-MALS), dynamic light scattering (DLS), electrospray differential mobility analysis (ES-DMA) and high performance size exclusion chromatography (HPSEC) [69,112].…”

Section: Characterization Of Vlpsmentioning

confidence: 99%

Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers

et al. 2021

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Virus-like particles (VLPs) are virus-derived structures made up of one or more different molecules with the ability to self-assemble, mimicking the form and size of a virus particle but lacking the genetic material so they are not capable of infecting the host cell. Expression and self-assembly of the viral structural proteins can take place in various living or cell-free expression systems after which the viral structures can be assembled and reconstructed. VLPs are gaining in popularity in the field of preventive medicine and to date, a wide range of VLP-based candidate vaccines have been developed for immunization against various infectious agents, the latest of which is the vaccine against SARS-CoV-2, the efficacy of which is being evaluated. VLPs are highly immunogenic and are able to elicit both the antibody- and cell-mediated immune responses by pathways different from those elicited by conventional inactivated viral vaccines. However, there are still many challenges to this surface display system that need to be addressed in the future. VLPs that are classified as subunit vaccines are subdivided into enveloped and non- enveloped subtypes both of which are discussed in this review article. VLPs have also recently received attention for their successful applications in targeted drug delivery and for use in gene therapy. The development of more effective and targeted forms of VLP by modification of the surface of the particles in such a way that they can be introduced into specific cells or tissues or increase their half-life in the host is likely to expand their use in the future. Recent advances in the production and fabrication of VLPs including the exploration of different types of expression systems for their development, as well as their applications as vaccines in the prevention of infectious diseases and cancers resulting from their interaction with, and mechanism of activation of, the humoral and cellular immune systems are discussed in this review.

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“…AFM can also be used to identify inactive virus particles [101,102]. In addition to particle size selection, the nanomechanical properties of such particles have been investigated.…”

Section: Atomic Force Microscopy (Afm)mentioning

confidence: 99%

Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example

Zhdanov,

Ivin,

Shishparenok

et al. 2023

BIOMED KHIM

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Traditional antiviral vaccines are currently created by inactivating the virus chemically, most often using formaldehyde or β-propiolactone. These approaches are not optimal since they negatively affect the safety of the antigenic determinants of the inactivated particles and require additional purification stages. The most promising platforms for creating vaccines are based on pseudoviruses, i.e., viruses that have completely preserved the outer shell (capsid), while losing the ability to reproduce owing to the destruction of the genome. The irradiation of viruses with electron beam is the optimal way to create pseudoviral particles. In this review, with the example of the poliovirus, the main algorithms that can be applied to characterize pseudoviral particles functionally and structurally in the process of creating a vaccine preparation are presented. These algorithms are, namely, the analysis of the degree of genome destruction and coimmunogenicity. The structure of the poliovirus and methods of its inactivation are considered. Methods for assessing residual infectivity and immunogenicity are proposed for the functional characterization of pseudoviruses. Genome integrity analysis approaches, atomic force and electron microscopy, surface plasmon resonance, and bioelectrochemical methods are crucial to structural characterization of the pseudovirus particles.

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Characterization of virus-like particles by atomic force microscopy in ambient conditions

Cited by 6 publications

References 17 publications

Identification of HIV-1–Based Virus-like Particles by Multifrequency Atomic Force Microscopy

Identification of HIV-1–Based Virus-like Particles by Multifrequency Atomic Force Microscopy

Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers

Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example

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