Characterization of voltage-activated ionic currents in the GnRH-containing terminalis nerve in transgenic zebrafish

Huang, Luoxiu; Li, Lei

doi:10.1016/j.brainres.2010.10.024

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…In developing transgenic fish, the GFP-tagged TNs can be readily identified and manipulated (Fig 2A) [23, 30]. Stimulation of the olfactory neurons (by 1.2 mM methionine, applied to the nostril) produced long-lasting effects on the TNs, such as increases in membrane potentials (Fig 2B).…”

Section: Resultsmentioning

confidence: 99%

“…To test if the changes of the animal’s sexual behaviors after odor stimulation may relate to olfacto-visual centrifugal GnRH signaling, i.e., if the TN cells respond to increases in glutamate concentrations after odor stimulation, we recorded the TNs in live animals in response to odor stimulation using transgenic zebrafish Tg(GnRH3::GFP). In developing transgenic fish, the GFP-tagged TNs can be readily identified and manipulated ( Fig 2A ) [ 23 , 30 ]. Stimulation of the olfactory neurons (by 1.2 mM methionine, applied to the nostril) produced long-lasting effects on the TNs, such as increases in membrane potentials ( Fig 2B ).…”

Section: Resultsmentioning

confidence: 99%

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GnRH-mediated olfactory and visual inputs promote mating-like behaviors in male zebrafish

Wojtowicz

Malin

et al. 2017

PLoS ONE

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The engagement of sexual behaviors is regulated by a number of factors which include gene expression, hormone circulation, and multi-sensory information integration. In zebrafish, when a male and a female are placed in the same container, they show mating-like behaviors regardless of whether they are kept together or separated by a net. No mating-like behaviors are observed when same-sex animals are put together. Through the olfacto-visual centrifugal pathway, activation of the terminalis nerve in the olfactory bulb increases GnRH signaling in the brain and triggers mating-like behaviors between males. In zebrafish mutants or wild-type fish in which the olfacto-visual centrifugal pathway is impaired or chemically ablated, in response to odor stimulation the mating-like behaviors between males are no longer evident. Together, the data suggest that the combination of olfactory and visual signals alter male zebrafish's mating-like behaviors via GnRH signaling.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

GnRH-mediated olfactory and visual inputs promote mating-like behaviors in male zebrafish

Wojtowicz

Malin

et al. 2017

PLoS ONE

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“…This whole brain in vitro preparation of small fish (DG and medaka) has since been successfully used by Oka and his colleagues ( 1 – 3 , 6 ), and Wayne and her colleagues ( 7 , 8 ). GFP-GnRH transgenic zebrafish have also been developed recently for the electrophysiological study of GnRH neurons ( 89 ). Since no other neurobiological studies on physiology and morphology of TN-GnRH3 neurons are available in mammals or other non-mammalian species, we will mainly review results of these studies obtained in the teleost fish brains.…”

Section: The Terminal Nerve-gnrh3 Neuronsmentioning

confidence: 99%

Neurobiological Study of Fish Brains Gives Insights into the Nature of Gonadotropin-Releasing Hormone 1–3 Neurons

Karigo

Oka

2013

Front. Endocrinol.

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Accumulating evidence suggests that up to three different molecular species of GnRH peptides encoded by different paralogs of gnrh genes are expressed by anatomically distinct groups of GnRH neurons in the brain of one vertebrate species. They are called gnrh1, gnrh2, and gnrh3. Recent evidence from molecular, anatomical, and physiological experiments strongly suggests that each GnRH system functions differently. Here, we review recent advancement in the functional studies of the three different GnRH neuron systems, mainly focusing on the electrophysiological analysis of the GnRH-green fluorescent protein (GFP) transgenic animals. The introduction of GFP-transgenic animals for the electrophysiological analysis of GnRH neurons greatly advanced our knowledge on their anatomy and electrophysiology, especially of gnrh1 neurons, which has long defied detailed electrophysiological analysis of single neurons because of their small size and scattered distribution. Based on the results of recent studies, we propose that different electrophysiological properties, especially the spontaneous patterns of electrical activities and their time-dependent changes, and the axonal projections characterize the different functions of GnRH1-3 neurons; GnRH1 neurons act as hypophysiotropic neuroendocrine regulators, and GnRH2 and GnRH3 neurons act as neuromodulators in wide areas of the brain.

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“…The effect of TN signaling on DA-IPCs has also been examined. In zebrafish, for example, stimulation of olfactory neurons alters the activity of TNs, which in turn decreases dopamine release, lifts the inhibition to RGCs, and increases inner retinal sensitivity [20,21,22]. …”

Section: Introductionmentioning

confidence: 99%

Zebrafish Olfacto-Retinal Centrifugal Axon Projection and Distribution: Effects of Gonadotropin-Releasing Hormone and Dopaminergic Signaling

Pfister

Yu²,

Kim³

et al. 2015

Dev Neurosci

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The terminalis neurons (TNs) have been described in teleost species. In zebrafish, the TNs are located in the olfactory bulb. The TNs synthesize and release gonadotropin-releasing hormone (GnRH) as one of the major neurotransmitters. The TNs project axons to many brain areas, which include the neural retina. In the retina, the TN axons synapse with dopaminergic interplexiform cells (DA-IPCs) and retinal ganglion cells (RGCs). In this research, we examine the role of GnRH and dopaminergic signaling in TN axon projection to the retina using the transgenic zebrafish Tg(GnRH-3::GFP). While the TNs developed at 34 h postfertilization (hpf), the first TN axons were not detected in the retina until 48-50 hpf, when the first DA-IPCs were differentiated. In developing embryos, inhibition of retinal GnRH signaling pathways severely interrupted the projection of TN axons to the retina. However, inhibition of retinal dopaminergic signaling produced little effect on TN axon projection. In adult retinas, inactivation of GnRH receptors disrupted the patterns of TN axon distribution, and depletion of DA-IPCs abolished the TN axons. When DA-IPCs regenerated, the TN axons reappeared. Together, the data suggest that in developing zebrafish retinas GnRH signaling is required for TN axon projection, whereas in adult retinas activation of GnRH and dopaminergic signaling transduction is required for normal distribution of the TN axons.

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Characterization of voltage-activated ionic currents in the GnRH-containing terminalis nerve in transgenic zebrafish

Cited by 5 publications

References 35 publications

GnRH-mediated olfactory and visual inputs promote mating-like behaviors in male zebrafish

GnRH-mediated olfactory and visual inputs promote mating-like behaviors in male zebrafish

Neurobiological Study of Fish Brains Gives Insights into the Nature of Gonadotropin-Releasing Hormone 1–3 Neurons

Zebrafish Olfacto-Retinal Centrifugal Axon Projection and Distribution: Effects of Gonadotropin-Releasing Hormone and Dopaminergic Signaling

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