Characterization of waste cell biomass derived glutamate decarboxylase for in vitro γ-aminobutyric acid production and value-addition

Wu, Chien-Hui; Dong, Cheng-Di; Patel, Anil Kumar; Singhania, Reeta Rani; Yang, Mingjie; Guo, Huei-Rong; Kuo, Jen‐Min

doi:10.1016/j.biortech.2021.125423

Cited by 10 publications

(3 citation statements)

References 36 publications

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“…brevis is achieved through the glutamate decarboxylase (GAD, EC 4.1.1.15) system under acidic conditions. 30 The GAD system consists of the GAD and glutamate/GABA antiporter (GadC). 31 Glutamate is transported into the cytosol via GadC and performs an irreversible decarboxylation reaction catalyzed by GAD to produce GABA, and the GABA is then mediums (FM1, FM2, FM3, FM4, FM5, FM6, and FM7) listed in Table S1 were used for GABA production.…”

Section: Introductionmentioning

confidence: 99%

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High Production of γ-Aminobutyric Acid by Activating the xyl Operon of Lactobacillus brevis

Cha

Ding

et al. 2023

ACS Omega

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γ-Aminobutyric acid (GABA) is an inhibitory neurotransmitter with important physiological functions such as sleep assistance and anti-depression. In this study, we developed a fermentation process for the high-efficiency production of GABA by Lactobacillus brevis (Lb. brevis) CE701. First, xylose was found as the optimal carbon source that could improve the GABA production and OD 600 in shake flasks to 40.35 g/L and 8.64, respectively, which were 1.78-fold and 1.67-fold of the glucose. Subsequently, the analysis of the carbon source metabolic pathway indicated that xylose activated the expression of the xyl operon, and xylose metabolism produced more ATP and organic acids than glucose, which significantly promoted the growth and GABA production of Lb. brevis CE701. Then, an efficient GABA fermentation process was developed by optimizing the medium components using response surface methodology. Finally, the production of GABA reached 176.04 g/L in a 5 L fermenter, which was 336% higher than that in a shake flask. This work enables the efficient synthesis of GABA using xylose, which will provide guidance for the industrial production of GABA.

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Section: Introductionmentioning

confidence: 99%

“…GABA synthesis by Lb. brevis is achieved through the glutamate decarboxylase (GAD, EC 4.1.1.15) system under acidic conditions . The GAD system consists of the GAD and glutamate/GABA antiporter (GadC) .…”

Section: Introductionmentioning

confidence: 99%

High Production of γ-Aminobutyric Acid by Activating the xyl Operon of Lactobacillus brevis

Cha

Ding

et al. 2023

ACS Omega

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“…LAB is beneficial to our health because they ferment many foodstuff-producing products that lower blood sugar and exert anti-inflammatory actions. Thus, they are useful in many applications such as food processing, medicinal development, and cosmetic and other chemical industries [33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Development of Blood Sugar Regulatory Products from Momordica cochininensis via Probiotic Fermentation

Guo²,

Liu

et al. 2023

Fermentation

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Type II diabetes is the most important health issue in the whole world. Besides the use of prescribed drugs to control blood glucose level, recently, the development of health supplements is being actively explored. Owing to its high nutritional value, Momordica cochinchinensis Spreng. (Gac) is potentially a good source for developing this supplement. In recent years, the aril of Gac has been utilized as a substrate for developing various forms of supplements, but the pulp has been neglected as a byproduct. However, the pulp contains lots of phytochemicals that could provide health benefits, and the investigation using lactobacilli to ferment the pulp juices to lower blood glucose is not yet to be explored. Therefore, we set out to investigate the potential to develop the pulp-based juices for controlling blood glucose level by selecting an optimal strain of lactobacillus to ferment the pulp juice and measuring the inhibitory action of the fermented juice on α-glucosidase. This enzyme is crucial for controlling postprandial glucose absorbed into the bloodstream because it is the enzyme that hydrolyzes the carbohydrates to release glucose. First, we have successfully isolated a strain of lactobacillus which was capable of fermenting the pulp to produce α-glucosidase-inhibitory activity. Through a 16S rRNA sequence, this lactobacillus was named Lactiplantibacillus plantarum GBI 001. The optimal conditions for its growth in commercial culture medium were found to be 35 °C for 16 h to produce the highest α-glucosidase activity (72.03%). The optimal conditions for the strain to grow in Gac pulp juice were: 20% pulp juices as substrate with an initial pH adjusted to 4.0, growing at 35 °C for 16 h. Under these conditions, the fermented juice exhibited α-glucosidase activity of 24.36%, which is a 2.17-fold increase over the control group (11.23%). From its increase in α-glucosidase potency, using L. plantarum GBI 001 to ferment the pulp juices of Gac as soft drinks has great potential to develop a helpful drink as a food supplement to control postprandial blood glucose in patients with diabetes.

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Novel food isolates with striking α-glucosidase inhibitory activity and probiotic potential for an antidiabetic role

Guo,

Singhania,

Patel

et al. 2023

J Food Sci Technol

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Characterization of waste cell biomass derived glutamate decarboxylase for in vitro γ-aminobutyric acid production and value-addition

Cited by 10 publications

References 36 publications

High Production of γ-Aminobutyric Acid by Activating the xyl Operon of Lactobacillus brevis

High Production of γ-Aminobutyric Acid by Activating the xyl Operon of Lactobacillus brevis

Development of Blood Sugar Regulatory Products from Momordica cochininensis via Probiotic Fermentation

Novel food isolates with striking α-glucosidase inhibitory activity and probiotic potential for an antidiabetic role

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