Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis–gas chromatography

Yokoi, Haruhiko; Ishida, Yasuhiro; Ohtani, Hajime; Tsuge, Shin; Sonoda, Tsutomu; Ona, Toshihiro

doi:10.1039/a809217f

Cited by 55 publications

(38 citation statements)

References 15 publications

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“…An increase of lignin S-G ratio with plant maturity has also been reported after Py-GC-MS of nonwoody fibers (Mazumder et al, 2005). This difference in timing of monolignol deposition would also be responsible for the within-tree variation of the S-G ratio observed in Eucalyptus camaldulensis wood (Ona et al, 1997;Yokoi et al, 1999).…”

Section: Py-gc-ms Of Whole Woods and Their Isolated Ligninsmentioning

confidence: 82%

“…Py-GC-MS, although not a fully quantitative technique, has been successfully used to analyze the relative H-G-S composition of lignin in different hardwoods, including eucalypt wood (Rodrigues et al, 1999;Yokoi et al, 1999Yokoi et al, , 2001del Río et al, 2005;Rencoret et al, 2007Rencoret et al, , 2008. Pyrograms from the eucalypt wood samples after different growth periods and their corresponding MWLs are shown in Figures 1 and 2, and the identities and relative molar abundances of the released lignin-derived compounds are listed in Table II. The pyrolysis of the different eucalypt woods released both carbohydrate-and lignin-derived compounds.…”

Section: Py-gc-ms Of Whole Woods and Their Isolated Ligninsmentioning

confidence: 99%

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Lignin Composition and Structure in Young versus AdultEucalyptus globulusPlants

et al. 2010

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Lignin changes during plant growth were investigated in a selected Eucalyptus globulus clone. The lignin composition and structure were studied in situ by a new procedure enabling the acquisition of two-dimensional nuclear magnetic resonance (2D-NMR) spectra on wood gels formed in the NMR tube as well as by analytical pyrolysis-gas chromatography-mass spectrometry. In addition, milled-wood lignins were isolated and analyzed by 2D-NMR, pyrolysis-gas chromatography-mass spectrometry, and thioacidolysis. The data indicated that p-hydroxyphenyl and guaiacyl units are deposited at the earlier stages, whereas the woods are enriched in syringyl (S) lignin during late lignification. Wood 2D-NMR showed that b-O-4# and resinol linkages were predominant in the eucalypt lignin, whereas other substructures were present in much lower amounts. Interestingly, open b-1# structures could be detected in the isolated lignins. Phenylcoumarans and cinnamyl end groups were depleted with age, spirodienone abundance increased, and the main substructures (b-O-4# and resinols) were scarcely modified. Thioacidolysis revealed a higher predominance of S units in the ether-linked lignin than in the total lignin and, in agreement with NMR, also indicated that resinols are the most important nonether linkages. Dimer analysis showed that most of the resinol-type structures comprised two S units (syringaresinol), the crossed guaiacyl-S resinol appearing as a minor substructure and pinoresinol being totally absent. Changes in hemicelluloses were also shown by the 2D-NMR spectra of the wood gels without polysaccharide isolation. These include decreases of methyl galacturonosyl, arabinosyl, and galactosyl (anomeric) signals, assigned to pectin and related neutral polysaccharides, and increases of xylosyl (which are approximately 50% acetylated) and 4-O-methylglucuronosyl signals.

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Section: Py-gc-ms Of Whole Woods and Their Isolated Ligninsmentioning

confidence: 82%

Section: Py-gc-ms Of Whole Woods and Their Isolated Ligninsmentioning

confidence: 99%

Lignin Composition and Structure in Young versus AdultEucalyptus globulusPlants

et al. 2010

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“…However, besides E. globulus, other eucalypt species are also used for papermaking, but their chemical compositions have not been characterized. Only some limited information regarding the composition of lipophilic extractives from E. grandis and E. urograndis wood (Freire et al 2006) and the lignin composition of E. camaldulensis and E. grandis (Ona et al 1997;Gonzá lez-Vila et al 1999;Yokoi et al 1999Yokoi et al , 2001Capanema et al 2005) is available.…”

Section: Introductionmentioning

confidence: 99%

“…Py-GC/MS is a rapid and highly sensitive technique for characterizing the chemical structure of lignin in low amounts of wood (Faix et al 1990;Ralph and Hatfield 1991). Py-GC/MS has already been successfully used for the calculation of lignin S/G ratios in wood, including woods from different eucalypt species (Rodrigues et al 1999;Yokoi et al 1999Yokoi et al , 2001del Río et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Lipid and lignin composition of woods from different eucalypt species

2007

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The lipid and lignin compositions of woods from the eucalypt species Eucalyptus globulus, E. nitens, E. maidenii, E. grandis, and E. dunnii have been characterized. The lipid composition was analyzed by GC and GC/MS using short-and medium-length high-temperature capillary columns, which allowed the detection of intact highmolecular-weight compounds. Similar lipid compositions were observed in all eucalypt woods, which were dominated by sitosterol, sitosterol esters and sitosteryl 3b-Dglucopyranoside. These substance classes are mainly responsible for pitch deposition during kraft pulping of eucalypt wood. However, some quantitative differences were found in the abundance of different lipid classes, with wood from E. globulus containing the lowest amounts of these pitch-forming compounds. The lignins of all eucalypt woods were analyzed in situ (without previous isolation) by pyrolysis-GC/MS. A predominance of syringyl (S) over guaiacyl (G) lignin units was observed and the S/G ratio was in the range from 2.7 to 4.1. E. globulus wood had the highest S/G ratio, a finding that, together with its low lignin content, explains its easy delignification under kraft cooking conditions.

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“…Come risultato della Py-GC-MS, è stato prodotto un pirogramma, dal quale è risultato possibile individuare quattordici picchi attribuibili ai fenoli costituenti della lignina (Terron et al, 1995;Del Rio et al, 2001;Yokoi et al, 1999). Il materiale legnoso pirolizzato infatti produce una miscela di fenoli semplici, risultanti dalla scissione dei legami C-O-C e C-C tra le unità fenoliche della lignina, e di eterocicli di tipo furanico derivanti dalla degradazione termica dei carboidrati.…”

Section: Pirolisi-gascromatografia-spettrometria DI Massa E Analisi Dunclassified

Management and estimate of forest utilization value in Piedmont

Brun¹,

Mosso²,

Blanc³

2015

Atti Del Secondo Congresso Internazionale Di Selvicoltura = Proceedings of the Second International Congress of Silviculture

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Quanto esposto è di esclusiva proprietà scientifica e intellettuale degli Autori ed esclude ogni responsabilità del curatore e dell'Editore. Intellectual and scientific property is exclusively of the authors of each contribution and does not entail responsibility of the editor and the publisher Con il patrocinio di / Under the Patronage of-Presidenza del Consiglio dei Ministri -Ministero delle politiche agricole alimentari e forestali -Ministero dei beni e delle attività culturali e del turismo -Ministero dell'ambiente e della tutela del territorio e del mare -Conferenza delle Regioni e delle Province Autonome -Expo Milano 2015 -La Toscana verso EXPO -Toscana Promozione -Università di Firenze -Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Progetto Integral -Istituto Nazionale di Economia Agraria -GESAAF-Dipartimento di gestione dei sistemi agrari, alimentari e forestali dell'università di Firenze -Provincia di Firenze -Comune di Firenze -Accademia dei Georgofili -Consiglio dell'Ordine Nazionale dei dottori agronomi e dei Dottori Forestali (CONAF) -Società Italiana di Selvicoltura ed Ecologia Forestale (SISEF) -Unione Nazionale delle Accademie per le Scienze Applicate allo Sviluppo dell'Agricoltura, alla Sicurezza Alimentare ed alla Tutela Ambientale (UNASA) -Unione Zoologica Italiana Con il contributo di / SESSION 1 -ECOLOGY, BIODIVERSITY, GENETICS AND FOREST ADAPTATION PROCESSES TO CLIMATE CHANGE Chairpersons Marco Borghetti, Raffaello GianniniRelazioni Orali / Oral presentations Paffetti D., Fiorentini S., Vettori C., Bottalico F., Buonamici A., Maltoni A., Nocentini S., Giannini R., Travaglini D. -La struttura genetica spaziale della diversità genetica può essere utilizzata come indicatore di vetustà dei boschi di faggio? Primi risultati in Italia centrale - » 208 Lombardi F., Parisi F., Sciarretta A., Campanaro A., Tognetti R., Chirici G., Trematerra P., Marchetti M. -Relazioni tra struttura forestale, indicatori di naturalità e fauna saproxilica: un caso studio nell'abetina di "Abeti Soprani" (Molise) After a brief presentation of the policy and scientific debate on the concept of governance as applied to the forestry sector, the paper focuses on 5 statements: (1) the transition from conventional forms of government to new modes of governance is a very progressive process, and never a complete change; (2) the integration of the stakeholders and the public in decision-making processes still provides the main characteristic of governance systems as they presently work; (3) curiously, searching for"good" governance usually leads to a clear re-consideration of the importance of the traditional role of the State, which was never considered as an initial objective; (4) monitoring governance requires specific assessment framework for follow-up; (5) governance does not necessarily lead to sustainability (and vice-versa). Considering that both forest and forestry form a social-ecological system, the presentation calls for re-defining forest action as a process for adapting the objective of sustaina...

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Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis–gas chromatography

Cited by 55 publications

References 15 publications

Lignin Composition and Structure in Young versus AdultEucalyptus globulusPlants

Lignin Composition and Structure in Young versus AdultEucalyptus globulusPlants

Lipid and lignin composition of woods from different eucalypt species

Management and estimate of forest utilization value in Piedmont

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