Characterization of xylose reductase extracted by CTAB-reversed micelles from Candida guilliermondii homogenate

Abstract

Xylosereductase (XR)(

Production of Xylitol from Agricultural Waste by Enzymatic Methods

“…Here we showed a decrease in enzyme activity at 40°C compared to temperatures of 30°C to 35°C. Cortez et al (2006) did see differences in the XR after reverse micelle purification, presumable due to the effects of the organic solvents (isooctane and hexanol) used in the reverse micelle procedure.…”

“…The XR activity in the crude extract was determined by incubating 100 µL of 5 mM xylose, 10 mM NADPH, 250 µL of enzyme extract and 600 µL of 50 mM phosphate buffer (pH 7). The reaction was carried out in 1 mL spectrophotometer cell at room temperature and the CXR activity was determined spectrophotometrically at 340 nm at room temperature according to Cortez et al (2006). One unit of XR was defined as the amount of enzyme required to produce one µmol of NADP per minute, which is equivalent to one micro mole of xylitol.…”

“…Branco et al (2011) used a reverse micelle technique to partially purify XR and synthesized xylitol from xylose at 25°C at pH 7. Cortez et al (2006) also used the reverse micelle technique to partially purify XR from Candida guillermondii. They showed that the enzyme had an optimum pH at 6.0 and a high activity range from pH 5.6 to 6.5 which is similar to what we show here.…”