Characterization of Yak Common Biofluids Metabolome by Means of Proton Nuclear Magnetic Resonance Spectroscopy

Zhu, Chenglin; Li, Cheng; Wang, Yaning; Laghi, Luca

doi:10.3390/metabo9030041

Cited by 25 publications

(33 citation statements)

References 40 publications

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“…An example of this possibility is offered by allantoin. This molecule is the fourth most concentrated in giraffe urine (Table S1), identically to yak (Bos grunniens) [17] and horse [18]. Differently from these strictly herbivorous animals, this molecule is the most concentrated in the urine of the giant panda [19], even if the giant panda consumes an amount of vegetables in relation to body weight (as much as 30%) much higher than ruminants or horses, which should lead to the lowest concentration of urinary allantoin [20].…”

Section: Discussionmentioning

confidence: 96%

“…According to Zhu et al [17], we suppressed the signals from broad resonances using a CPMG-(Carr-Purcell-Meiboom-Gill) filter composed of 400 echoes with a of 400 s and a 180 • pulse of 24 s, for a total filter of 330 ms. We also applied pre-saturation, to reduce the signal from water. We employed Topspin software to apply a line broadening of 0.3 Hz and to adjust the phase of each spectrum.…”

Section: Metabolomic Analysismentioning

confidence: 99%

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First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

et al. 2020

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The urine from 35 giraffes was studied by untargeted 1H-NMR, with the purpose of obtaining, for the first time, a fingerprint of its metabolome. The metabolome, as downstream of the transcriptome and proteome, has been considered as the most representative approach to monitor the relationships between animal physiological features and environment. Thirty-nine molecules were unambiguously quantified, able to give information about diet, proteins digestion, energy generation, and gut-microbial co-metabolism. The samples collected allowed study of the effects of age and sex on the giraffe urinary metabolome. In addition, preliminary information about how sampling procedure and pregnancy could affect a giraffe’s urinary metabolome was obtained. Such work could trigger the setting up of methods to non-invasively study the health status of giraffes, which is utterly needed, considering that anesthetic-related complications make their immobilization a very risky practice.

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Section: Discussionmentioning

confidence: 96%

Section: Metabolomic Analysismentioning

confidence: 99%

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

et al. 2020

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“…For the metabolomics investigation by 1 H-NMR, an analysis solution was created, with 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid sodium salt (TSP) 10 mM in D 2 O, set at pH 7.00 ± 0.02 by means of 1 M phosphate buffer. The solution contained also 10 μL of NaN 3 2 mM, to avoid microbial proliferation, while TSP was employed as the 1 H NMR chemical-shift reference, as suggested by Zhu et al [ 17 ]. Growth medium samples were prepared for 1 H-NMR by thawing and centrifuging 1 mL of each for 15 min at 18,630 g and 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Metabolism of Lactobacillus sakei Chr82 in the Presence of Different Amounts of Fermentable Sugars

Barbieri

Laghi

Gardini

et al. 2020

Foods

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Lactobacillus sakei is widely used as a starter culture in fermented sausages since it is well adapted to meat environments and able to maintain high viability thanks to secondary pathways activated when hexoses are depleted (i.e., metabolism of pentoses and amino acids). In this study, a commercial strain of L. sakei was inoculated in a defined medium with ribose or glucose as the carbon source, at optimal or reduced concentrations, to evaluate its different physiological and metabolic responses in relation to different growth conditions. The results obtained with different approaches (HPLC, 1H-NMR, flow cytometry) evidenced different growth performances, amino acid consumptions and physiological states of cells in relation to the carbon source as an active response to harsh conditions. As expected, higher concentrations of sugars induced higher growth performances and the accumulation of organic acids. The low sugars amount induced the presence of dead cells, while injured cells increased with ribose. Arginine was the main amino acid depleted, especially in the presence of higher ribose, and resulted in the production of ornithine. Moreover, the 1H-NMR analysis evidenced a higher consumption of serine at the optimal sugars concentration (pyruvate production). This information can be helpful to optimize the use of these species in the industrial production of fermented sausages.

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“…Metabolic pathways analysis was performed using a Bos taurus pathway library. Metabolic pathways were common quantified, and different metabolites in biofluid metabolites of the other studied animals were statistically analyzed by MetaboAnalyst 4.0 for metabolic pathways analysis, which is based on database source by Kyoto Encyclopedia of Genes and Genomes [71].…”

Section: H-nmr Spectroscopy Data and Statistical Analysismentioning

confidence: 99%

Metabolomics Comparison of Hanwoo (Bos taurus coreanae) Biofluids Using Proton Nuclear Magnetic Resonance Spectroscopy

et al. 2020

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The aim of this study was to identify the metabolomic profiles of rumen fluid, serum, and urine from Hanwoo (Bos taurus coreanae), using proton nuclear magnetic resonance (1H-NMR) spectroscopy. In all, 189, 110, and 188 metabolites were identified in rumen fluid, serum, and urine, and 107, 49, and 99 were quantified, respectively. Organic acids, carbohydrates, and aliphatic acyclic compound metabolites were present at the highest concentrations in rumen fluid, serum, and urine, respectively. In addition, acetate, glucose, and urea were the most highly concentrated individual metabolites in rumen fluid, serum, and urine, respectively. In all, 77 metabolites were commonly identified, and 19 were quantified across three biofluids. Metabolic pathway analysis showed that the common quantified metabolites could provide relevant information about three main metabolic pathways, phenylalanine, tyrosine, and tryptophan biosynthesis; caffeine metabolism; and histidine metabolism. These results can be useful as reference values for future metabolomic research on Hanwoo biofluids in Korea.

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Characterization of Yak Common Biofluids Metabolome by Means of Proton Nuclear Magnetic Resonance Spectroscopy

Cited by 25 publications

References 40 publications

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

Metabolism of Lactobacillus sakei Chr82 in the Presence of Different Amounts of Fermentable Sugars

Metabolomics Comparison of Hanwoo (Bos taurus coreanae) Biofluids Using Proton Nuclear Magnetic Resonance Spectroscopy

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