Characterization of yeast in hapludands soil with biotechnological potential

Abstract

Yeasts play an important role in nature, and soil is one of the largest reservoirs of yeasts. Seventy-seven yeast strains were isolated from southern Chilean volcanic ash soil. These 77 strains were composed of 30 strains derived from soils used as permanent grassland, 30 strains from rotating grassland, and 17 strains from native forest (considered as the control). These strains were identified using restriction fragment length polymorphisms (PCR-RFLP) in conjunction with ITS-5.8S rDNA sequencing. Additionally, a physiological characterization of the assimilation of carbon and nitrogen and the fermentation of sugars was performed. Various features of the strains were evaluated including their growth in vinasse medium, nitrogen and phosphorus content, and lipid production. Lastly, the use of strains as biofertilizers was analyzed via the cultivation of bell pepper seedlings in soil. From the RFLP profiles, the 77 strains clustered into 10 groups. Of these, only three groups could be identified to the species level, and another was classified to the level of genus (Devaryomyces hansenii, Pichia fermentan, Kazachstania exigua, Candida sp.). All strains grew in vinasse medium. Six strains (PP1, PP4, PR4, PR10, PR27 and PR29) showed a high capacity to accumulate lipids, and three strains (PP1, PP21 and PR28) have the potential to be used as biofertilizers.

Evaluation of Multifarious Plant Growth Promoting Trials of Yeast Isolated from the Soil of Assam Tea (Camellia sinensis var. assamica) Plantations in Northern Thailand

“…assamica ) that had been collected in northern Thailand using the enrichment isolation technique. These results were in accordance with the findings of previous studies which reported that ascomycetous and basidiomycetous yeast strains were recovered from various soil samples [ 24 , 47 , 48 , 49 ]. Many ascomycetous yeast strains in the genera Aureobasidium , Barnettozyma , Cyberlindnera , Kazachstania , Lipomyces and Schwanniomyces are known to be generally more abundant and are more frequently found in soil [ 24 , 50 , 51 ].…”

Nothofagus pumilio forest affected by recent tephra deposition in northern Patagonia. II- Shifts in diversity and structure of rhizosphere fungal communities

“…Consequently, in terms of research these organisms represent one of the most interesting microbial groups in the soil environment. The fact that in Patagonia several studies are being carried out on mycorrhizas (Fontenla et al, 1998;Fernández et al, 2012;Nouhra et al, 2013;Fernández et al, 2015;Marín et al, 2016) and other soil fungal communities (Mestre et al, 2011;Díaz et al, 2017) reinforces the importance of describing these organisms in natural ecosystems.…”

Lipid Production from Native Oleaginous Yeasts Isolated from Southern Chilean Soil Cultivated in Industrial Vinasse Residues

“…and Apiotrichum dulcitum, respectively. Both genera have been described as capable of accumulating more than 25% of their dry cell weight in TG [49]. Debaryomyces hansenii is a non-conventional yeast considered to be a well-suited option for a number of different industrial bioprocesses.…”

“…Six yeast strains named PP1, PP4, PR4, PR10, PR27 and PR29 were included in this study. These strains were isolated from soil of the Los Ríos region, Chile, and showed biotechnological potential for lipid accumulation in a previous study by Díaz et al [49]. The strains were maintained in sabouraud agar slants at 4 • C with subcultures being performed every three months.…”