Characterization of yeast population from unstudied natural sources in La Mancha region

Abstract

Aims This study aims to identify the yeast species and strains which entitled an unstudied area of Spain and evaluate the yeast species diversity richness and the genetic variety. Methods and results A total of 702 yeasts were isolated from different environments in a central Spanish region (La Mancha) with diverse sources of origin (food, animals, flowers and environmental sources) during spring season. Thanks to the analysis carried out by the PCR‐RFLP technique and sequencing, 35 species were identified. A neighbour‐joining phylogenetic tree was created based on D1/D2 sequences. Moreover 330 strains were determined by PCR‐RAPD and their profiles were analysed using the bioinformatics programme BioNumerics 7·6. The Simpson’s index (D) and the genetic diversity percentage were calculated with the aim of studying the richness of the species in each environment and the genetic variety in each species. Conclusions This study has permitted to know that the majority of the species found was Diutina rugosa while the most ubiquitous was Rhodotorula mucilaginosa which expose the dispersion capability of this species. The diversity parameters has revealed that the highest species richness was associated to environmental samples and the highest genetic variety was presented in those species with better dispersion capability or a smaller number of isolates. Significance and Impact of the Study This study permits to better understand the yeast communities in La Mancha region which gives a value the microbial potential of this region.

Proteomic profiling and glycomic analysis of the yeast cell wall in strains with Aflatoxin B1 elimination ability

“…They were environmental isolates of Aureobasidium pullulans (FH1), Candida parapsilosis (ECF42), Wickerhamiella sorbophila (ECF12; formerly Candida sorbophila ), Candida tropicalis (AK11), Diutina rugosa (FR19 and ECF61), Rhodotorula mucilaginosa (EB39) and Saccharomyces cerevisiae (EB83). All these strains were catalogued and are kept in the University of Castilla – La Mancha (UCLM) yeast collection (García‐Béjar et al ., 2020b).…”

An alternative application of some SSR DNA markers in experimental mycology

“…Відмічаються суттєві труднощі з повторюванням результатів дослідів різними лабораторіями. Однак незважаючи на великі проблеми цього методу ПЛР, його продовжують широко застосовувати як для генетичної ідентифікації зразків (Wyss, 1996;Stefańska et al, 2022;Valencia-Ledezma et al, 2022), так і в популяційних дослідженнях (Czyzewska et al, 2018;García-Béjar et al, 2021;Wojciechowska-Koszko et al, 2022).…”

Untitled

“…Відмічаються суттєві труднощі з повторюванням результатів дослідів різними лабораторіями. Однак незважаючи на великі проблеми цього методу ПЛР, його продовжу ють широко застосовувати як для генетичної ідентифікації зразків (Wyss, 1996;Stefańska et al, 2022;ValenciaLedezma et al, 2022), так і в популяційних дослідженнях (Czyzewska et al, 2018;GarcíaBéjar et al, 2021;Wojciechows kaKoszko et al, 2022).…”