Characterization of zearalenone-induced hepatotoxicity and its mechanisms by transcriptomics in zebrafish model

Zhang, Changqing; Li, Chenqinyao; Liu, Kechun; Zhang, Yun

doi:10.1016/j.chemosphere.2022.136637

Cited by 12 publications

(8 citation statements)

References 56 publications

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Section: Resultsmentioning

confidence: 99%

“…ALT and AST are essential intracellular enzymes that are concentrated in hepatocytes and serve as valuable biomarkers to assess cellular and structural damage and functional failure in the liver. 22 During the exposure period, the activities of AST and ALT were significantly increased, especially by day 28 after exposure, implying cellular damage in the zebrafish liver (Figure 1e−f). Our results are comparable with those of Lucio et al 23 who confirmed that prolonged occupational exposure to low concentrations of anesthetic gases induced alternations in liver markers (AST, ALT, GGT, and bilirubin) and immunological parameters.…”

Section: Propofol Aggravated Histopathological Damagementioning

confidence: 97%

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Environmentally Relevant Concentrations of Propofol-Induced Metabolic Dysfunction in Zebrafish: Mechanistic Insights into Integrated Hepatic Transcriptomic and Metabolomic Analyses

Jiang,

Li,

Zhang

et al. 2024

ACS EST Water

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Propofol is increasingly perceived as an emerging threat to aquatic ecosystems, although the ecotoxicity and underlying mechanisms of propofol on aquatic life have not yet been clearly established. In this study, zebrafish (Danio rerio) was exposed to different concentrations of propofol (0.008, 0.04, and 0.2 mg•L −1 ) to probe the effect of propofol on functional metabolism and the ecological response mechanism in aquatic organisms. Propofol induced severe hepatic damage, including increased interstitial spaces and nuclear malformations at the tissue level. Reactive oxygen species (ROS) production and lipid peroxidation in zebrafish were induced after 28 days of exposure, while the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were substantially increased, suggesting that propofol may influence hepatic tissue injury through exacerbating oxidative damage. Exacerbated levels of triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) were indicative of dysregulated lipid metabolism. Comprehensive transcriptomics and metabolomics analyses showed that propofol mainly changed the glycerophospholipid metabolism, citric acid cycle, and purine metabolism, and the disorders of these pathways can aggravate lipid accumulation and oxidative damage. Overall, this study revealed the mechanisms of hepatotoxicity and metabolic dysfunction of propofol on zebrafish, providing constructive insights for a comprehensive assessment of the toxicity risk posed by propofol to aquatic environmental health.

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Section: Resultsmentioning

confidence: 99%

Section: Propofol Aggravated Histopathological Damagementioning

confidence: 97%

Environmentally Relevant Concentrations of Propofol-Induced Metabolic Dysfunction in Zebrafish: Mechanistic Insights into Integrated Hepatic Transcriptomic and Metabolomic Analyses

Jiang,

Li,

Zhang

et al. 2024

ACS EST Water

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“…Extending previous studies of ZEA in the zebrafish embryo model [ 12 , 19 , 30 , 31 ], the present study investigated the toxicity of ZEA in embryonic stages of not only zebrafish but two additional marine species, namely olive flounder and yellowtail snapper. Additionally, it is, indeed, the first study to assess toxicity in these two ecologically and economically relevant species.…”

Section: Discussionmentioning

confidence: 99%

High-Resolution Magic Angle Spinning (HRMAS) NMR Identifies Oxidative Stress and Impairment of Energy Metabolism by Zearalenone in Embryonic Stages of Zebrafish (Danio rerio), Olive Flounder (Paralichthys olivaceus) and Yellowtail Snapper (Ocyurus chrysurus)

Annunziato

Bashirova

Eeza

et al. 2023

Toxins

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Zearalenone (ZEA) is a mycotoxin, commonly found in agricultural products, linked to adverse health impacts in humans and livestock. However, less is known regarding effects on fish as both ecological receptors and economically relevant “receptors” through contamination of aquaculture feeds. In the present study, a metabolomics approach utilizing high-resolution magic angle spinning nuclear magnetic resonance (HRMAS NMR) was applied to intact embryos of zebrafish (Danio rerio), and two marine fish species, olive flounder (Paralichthys olivaceus) and yellowtail snapper (Ocyurus chrysurus), to investigate the biochemical pathways altered by ZEA exposure. Following the assessment of embryotoxicity, metabolic profiling of embryos exposed to sub-lethal concentrations showed significant overlap between the three species and, specifically, identified metabolites linked to hepatocytes, oxidative stress, membrane disruption, mitochondrial dysfunction, and impaired energy metabolism. These findings were further supported by analyses of tissue-specific production of reactive oxygen species (ROS) and lipidomics profiling and enabled an integrated model of ZEA toxicity in the early life stages of marine and freshwater fish species. The metabolic pathways and targets identified may, furthermore, serve as potential biomarkers for monitoring ZEA exposure and effects in fish in relation to ecotoxicology and aquaculture.

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“…Besides the superiorities of rapid development and cost-effectiveness, zebrafish also possess a high degree of physiological and genetic similarity to humans, especially regarding hepatic cellular composition, function, and the underlying cellular processes [ 19 ]. More remarkably, the liver-specific fluorescent transgenic lines allow for precise and direct in vivo visualization, accelerating the research of disease pathogenesis as well as the advancement of therapeutic strategies [ 20 , 21 ]. The multi-omics analysis, which can be utilized on zebrafish model, has been recognized as an increasingly powerful tool in liver disease research.…”

Section: Introductionmentioning

confidence: 99%

Integrated spatial metabolomics and transcriptomics decipher the hepatoprotection mechanisms of wedelolactone and demethylwedelolactone on non-alcoholic fatty liver disease

Chen,

Zhu,

Geng

et al. 2024

Journal of Pharmaceutical Analysis

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Characterization of zearalenone-induced hepatotoxicity and its mechanisms by transcriptomics in zebrafish model

Cited by 12 publications

References 56 publications

Environmentally Relevant Concentrations of Propofol-Induced Metabolic Dysfunction in Zebrafish: Mechanistic Insights into Integrated Hepatic Transcriptomic and Metabolomic Analyses

Environmentally Relevant Concentrations of Propofol-Induced Metabolic Dysfunction in Zebrafish: Mechanistic Insights into Integrated Hepatic Transcriptomic and Metabolomic Analyses

High-Resolution Magic Angle Spinning (HRMAS) NMR Identifies Oxidative Stress and Impairment of Energy Metabolism by Zearalenone in Embryonic Stages of Zebrafish (Danio rerio), Olive Flounder (Paralichthys olivaceus) and Yellowtail Snapper (Ocyurus chrysurus)

Integrated spatial metabolomics and transcriptomics decipher the hepatoprotection mechanisms of wedelolactone and demethylwedelolactone on non-alcoholic fatty liver disease

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