Characterization of α-type carbonic anhydrase (CA) gene and subcellular localization of α-CA in the gametophytes of Saccharina japonica

Ye, R.-X.; Yu, Zhenzhen; Shi, Wenzheng; Gao, HongJian; Bi, Yan-Hui; Zhou, Zhigang

doi:10.1007/s10811-013-0221-2

Cited by 26 publications

(24 citation statements)

References 48 publications

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“…The same was recently reported in Saccharina japonica , where cDNA encoding α ‐CA was associated with the chloroplast envelopes and thylakoid membranes (Ye et al. ). Although the β ‐type CA was not found in the EST of Ulva , it is reported to be localized in the cytosol of another green alga Coccomyxa (Huang et al.…”

Section: Discussionsupporting

confidence: 79%

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Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth in Ulva rigida (Chlorophyta)

Rautenberger

Fernández

Strittmatter

et al. 2015

Ecology and Evolution

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Carbon physiology of a genetically identified Ulva rigida was investigated under different CO2(aq) and light levels. The study was designed to answer whether (1) light or exogenous inorganic carbon (Ci) pool is driving growth; and (2) elevated CO2(aq) concentration under ocean acidification (OA) will downregulate CAext-mediated dehydration and alter the stable carbon isotope (δ13C) signatures toward more CO2 use to support higher growth rate. At pHT 9.0 where CO2(aq) is <1 μmol L−1, inhibition of the known use mechanisms, that is, direct uptake through the AE port and CAext-mediated dehydration decreased net photosynthesis (NPS) by only 56–83%, leaving the carbon uptake mechanism for the remaining 17–44% of the NPS unaccounted. An in silico search for carbon-concentrating mechanism elements in expressed sequence tag libraries of Ulva found putative light-dependent transporters to which the remaining NPS can be attributed. The shift in δ13C signatures from –22‰ toward –10‰ under saturating light but not under elevated CO2(aq) suggest preference and substantial use to support photosynthesis and growth. U. rigida is Ci saturated, and growth was primarily controlled by light. Therefore, increased levels of CO2(aq) predicted for the future will not, in isolation, stimulate Ulva blooms.

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Section: Discussionsupporting

confidence: 79%

“…() and Ye et al. (), suggest that this α ‐CA isozyme is responsible for the internal

{HCO}_{3}^{-}

dehydration to provide the chloroplast with sufficient CO 2 for carbon fixation. The same was recently reported in Saccharina japonica , where cDNA encoding α ‐CA was associated with the chloroplast envelopes and thylakoid membranes (Ye et al.…”

Section: Discussionmentioning

confidence: 98%

Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth in Ulva rigida (Chlorophyta)

Rautenberger

Fernández

Strittmatter

et al. 2015

Ecology and Evolution

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“…To obtain more information on the 18S-5.8S-25S and 5S rDNAs of the kelp gametophytes, Southern blot analysis was conducted as described by Ye et al (2014). In this experiment, we chose 18S rDNA (synthesized by using the primer pair, 18S-F/18S-R, Table 1) and 5S rDNA (synthesized by using the primer pair, 5Ss-F/5Ss-R, Table 1) as probes.…”

Section: Southern Blot Analysismentioning

confidence: 99%

“…Aliquots of isolated DNA (approximately 2 μg per sample) were digested to completion at 37°C for 12-14 h independently by using NcoI and SalI. Agarose electrophoresis of the digested DNA samples, blotting onto a positively charged nylon membrane (Pall, USA), hybridization with the synthesized 18S and 5S rDNA probes, and signal visualization on an XBT-1 film (Kodak, USA) were performed as previously described (Ye et al 2014). …”

Section: Southern Blot Analysismentioning

confidence: 99%

Characterization and physical mapping of nuclear ribosomal RNA (rRNA) genes in the haploid gametophytes of Saccharina japonica (Phaeophyta)

et al. 2017

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Nuclear tandemly repeated ribosomal RNA genes (18S-5.8S-25S rDNA and 5S rDNA) have been proven to be excellent cytogenetic markers for karyotype analysis of various higher plants by using the fluorescence in situ hybridization (FISH) technique. To illustrate physical mapping of these rDNAs in brown seaweed, Saccharina japonica, chromosomes, an approximately 5400-bp transcription unit of 18S-5.8S-25S rDNA was assembled by cloning the 25S rDNA after paired-end sequencing of two screened clones from a bacterial artificial chromosome library of kelp gametophytes. In contrast to the conserved 18S-5.8S-25S rDNA and ITS1 in S. japonica, the 245-bp ITS2 was variable in sequence. The cloned 5S rDNA revealed that the 120-bp conserved coding region was separated by a diverse intergenic spacer sequence that were 250, 445, 905, or 1335 bp in length. On average, the 18S-5.8S-25S rDNA of kelp female and male gametophytes had 45 and 41 copies per haploid genome, respectively, as detected by quantitative real-time PCR, whereas the 5S rDNA had 2590 and 2648 copies, respectively. Southern hybridization with labeled probes of 18S rDNA or 5S rDNA demonstrated that kelp gametophytes possessed only one locus each of the 18S-5.8S-25S or 5S rDNAs. This was further confirmed by FISH analysis using the same labeled probes, thus illustrating that 18S-5.8S-25S rDNA is located at the intercalary region of chromosome 23, whereas 5S rDNA at the sub-telomeric region of chromosomes 27. The localization of these rDNAs using the FISH technique has facilitated the identification of individual chromosomes and karyotype analysis of this kelp.

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“…However, the sequence similarities between the three CA enzymes were so low that they were assumed to be formed through different evolutionary paths. Among them, β-CA exists mainly in the chloroplasts of bacteria, algae, and monocotyledon and dicotyledon plants [14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

Cloning of SjCA gene and its expression analysis on upland cottons

Kong

Zhao

et al. 2016

JBEI

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Acquisition of salt-tolerant genes from exogenous plants to improve cotton salt resistance has always been a hotspot of research on cotton salt resistance. However, the information regarding the method of conversion of living cotton pollens by portable gene gun technology is still little. Complete sequence information of the Carbonic Anhydrase (CA) gene was obtained from NCBI database, and its full ORF length sequence was cloned by RT-PCR technology. After the construction of pBI121-CA::GFP fusion expression vector, we used cotton pollens from upland cotton varieties Y-2067, ZA-23, and GZ-2, which have weaker autofluorescence, to conduct the research on the transient expression of cotton pollens in vivo via particle bombardment (gene gun technology). The results indicated that green fluorescence enhancement of the three kinds of cotton pollens was realized after the CA gene transformation, meaning that the CA gene expression level was increased. Besides, the salt tolerance germination ability of transgenic T1 seeds was also improved. Our research initially established a transient expression system of cotton pollen in vivo via particle bombardment technology, laying the theoretical foundation for further research of cotton genetic transformation and creation of cotton salt-tolerant germplasm.

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Characterization of α-type carbonic anhydrase (CA) gene and subcellular localization of α-CA in the gametophytes of Saccharina japonica

Cited by 26 publications

References 48 publications

Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth in Ulva rigida (Chlorophyta)

Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth in Ulva rigida (Chlorophyta)

Characterization and physical mapping of nuclear ribosomal RNA (rRNA) genes in the haploid gametophytes of Saccharina japonica (Phaeophyta)

Cloning of SjCA gene and its expression analysis on upland cottons

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