Characterization of α1, β1, and γ1 laminin subunits during rabbit fetal lung development

Durham, Paul L.; Snyder, Jeanne M.

doi:10.1002/aja.1002030404

Cited by 27 publications

(18 citation statements)

References 31 publications

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“…Stainings with anti FP-B and AP83 gave similar results in all tissues analyzed (except for kidney and small intestine; see Results). Furthermore, we used rabbit antiserum ISH-1 raised against EHS laminin reacting equally well with all three chains of mouse laminin-1 (Klein et al 1988); guinea pig polyclonal antibody directed against human placental laminin (isoform specificity of this antibody is unknown) (Durham and Snyder 1995); rat monoclonal antibody (MAb) 200 against the E3 domain of laminin ␣ 1 -chain (Sorokin et al 1992); rat MAb 8G11D10 against laminin ␣ 2 -chain (Schuler and Sorokin 1995); and rat MAb GoH3 recognizing the ␣ 6 -integrin subunit (Sonnenberg et al 1990). Bound antibodies were visualized using secondary antibodies conjugated with FITC or Cy3 (Jackson ImmunoResearch Laboratories; West Grove, PA).…”

Section: Immunofluorescencementioning

confidence: 99%

Distribution of Dystroglycan in Normal Adult Mouse Tissues

Durbeej

Henry

Ferletta

et al. 1998

J Histochem Cytochem.

172

138

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SUMMARY Dystroglycan is a cell surface protein which, in muscle, links the extracellular matrix protein laminin-2 to the intracellular cytoskeleton. Dystroglycan also binds laminin-1 and the binding occurs via the E3 fragment of laminin-1. Recently, it was found that dystroglycan is expressed in developing epithelial cells of the kidney. Moreover, antibodies against dystroglycan can perturb epithelial development in kidney organ culture. Therefore, dystroglycan may be an important receptor for cell-matrix interactions in non-muscle tissues. However, information about the tissue distribution of dystroglycan is limited, especially in adult tissues. Here we show that dystroglycan is present in epithelial cells in several non-muscle organs of adult mice. Dystroglycan is enriched towards the basal side of the epithelial cells that are in close contact with basement membranes. We suggest that dystroglycan is involved in linking basement membranes to epithelial and muscle cells. Dystroglycan may be important for the maintenance of tissue integrity.

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Section: Immunofluorescencementioning

confidence: 99%

Distribution of Dystroglycan in Normal Adult Mouse Tissues

Durbeej

Henry

Ferletta

et al. 1998

J Histochem Cytochem.

172

138

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“…Taken together, these results suggest that laminin ␣5 is a major ␣ chain of adult epithelial and/or endothelial basal laminae. Moreover, it seems possible that reports of ␣1-like immunoreactivity in tissues such as kidney, lung, and muscle (20,23,(25)(26)(27)(28), which contain little ␣1 RNA, reflect cross-reactivity of anti-␣1 antibodies with ␣5. We are currently preparing monospecific antibodies to evaluate this possibility.…”

Section: Laminin ␣5 28525mentioning

confidence: 99%

“…Consistent with laminin's trimeric structure, all basal laminae characterized to date contain at least one ␤ and at least one ␥ chain (19,20,(23)(24)(25)(26)(27)(28). For the ␣ chains, on the other hand, the situation is less clear.…”

mentioning

confidence: 99%

“…So far, however, all laminin chains sequenced resemble either A, B1, or B2, and all of the laminins purified are trimers containing an A-like, a B1-like, and a B2-like chain (6, 19 -22). Based on these findings, a new nomenclature has been adopted in which laminin chains are divided into ␣ (A-like), ␤ (B1-like) and ␥ (B2-like) subfamilies; A, M, B1, S, and B2 are now called ␣1, ␣2, ␤1, ␤2, and ␥1, respectively (6).Consistent with laminin's trimeric structure, all basal laminae characterized to date contain at least one ␤ and at least one ␥ chain (19,20,(23)(24)(25)(26)(27)(28). For the ␣ chains, on the other hand, the situation is less clear.…”

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confidence: 99%

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Molecular Cloning of a Novel Laminin Chain, α5, and Widespread Expression in Adult Mouse Tissues

Miner¹,

Lewis

Sanes

1995

Journal of Biological Chemistry

232

178

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We have identified a fifth member of the ␣ subfamily of vertebrate laminin chains. Sequence analysis revealed a close relationship of ␣5 to the only known Drosophila ␣ chain, suggesting that the ancestral ␣ gene was more similar to ␣5 than to ␣1-4. Analysis of RNA expression showed that ␣5 is widely expressed in adult tissues, with highest levels in lung, heart, and kidney. Our results suggest that ␣5 may be a major laminin chain of adult basal laminae.Laminins are major glycoproteins of basal laminae throughout the vertebrate body. Originally identified as structural components, it is now clear that laminins are also signaling molecules that regulate the proliferation, motility, and differentiation of the cells they contact (1-3). The first laminin discovered (4, 5), now called laminin-1 (6), is a trimer of related A, B1, and B2 chains (7-9). The subsequent discovery of the novel laminin chains S-laminin (10) and merosin-M (11) revealed that the laminins comprised a larger gene family than initially envisioned. More recently, four additional chains have been cloned (12)(13)(14)(15)(16)(17)(18)(19). So far, however, all laminin chains sequenced resemble either A, B1, or B2, and all of the laminins purified are trimers containing an A-like, a B1-like, and a B2-like chain (6, 19 -22). Based on these findings, a new nomenclature has been adopted in which laminin chains are divided into ␣ (A-like), ␤ (B1-like) and ␥ (B2-like) subfamilies; A, M, B1, S, and B2 are now called ␣1, ␣2, ␤1, ␤2, and ␥1, respectively (6).Consistent with laminin's trimeric structure, all basal laminae characterized to date contain at least one ␤ and at least one ␥ chain (19,20,(23)(24)(25)(26)(27)(28). For the ␣ chains, on the other hand, the situation is less clear. For example, perineurial basal lamina in peripheral nerve stained poorly with anti-␣1 and not at all with anti-␣2 (23). Likewise, in kidney, glomerular basement membrane was ␣2-negative and reacted only moderately well (in human (23)) or not at all (in mouse (29, 30)) with anti-␣1. If all laminins are ␣/␤/␥ trimers, these results imply that additional laminin ␣ chains exist. Indeed, several biochemical studies have provided evidence for an ␣-like laminin chain distinct from ␣1 and ␣2 (31-33). The recent discoveries of the ␣3 and ␣4 chains (15-18) are provocative in this context, but we 1 and others (16,18) found little ␣3 or ␣4 in several tissues with ␣1-and ␣2-negative basal laminae.Accordingly, we undertook a search for additional laminin ␣ chains. Using the polymerase chain reaction, we have identified laminin ␣5, a novel murine ␣ chain. Sequence analysis reveals that ␣5 is more similar in domain structure to Drosophila laminin A (34, 35) than it is to mammalian ␣1-4; the ancestral vertebrate ␣ chain may, therefore, have been more similar to ␣5 that to ␣1-4. RNA analysis demonstrates that ␣5 is widely expressed in adult tissues and thus may be a major laminin ␣ chain of adult basal laminae. MATERIALS AND METHODSDegenerate primers were designed based on sequences conserved bet...

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“…Laminin-␣1 and -␣5 are colocalized, but laminin-␣1 expression is restricted to the first trimester whereas laminin-␣5 expression is present at the end of the first trimester and continues throughout adulthood (30). All three laminin ␤-chains and laminin-␥1 and -␥2 are found in both fetal and adult lungs (10,11,37). Little is known about the effects of injury and disease on the expression of laminin chains in the lung.…”

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confidence: 99%

Laminin α-chain expression and basement membrane formation by MLE-15 respiratory epithelial cells

Nguyen

Bai

Mochitate

et al. 2002

American Journal of Physiology-Lung Cellular and Molecular Phys

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-Basement membranes have a critical role in alveolar structure and function. Alveolar type II cells make basement membrane constituents, including laminin, but relatively little is known about the production of basement membrane proteins by murine alveolar type II cells and a convenient system is not available to study basement membrane production by murine alveolar type II cells. To facilitate study of basement membrane production, with particular focus on laminin chains, we examined transformed murine distal respiratory epithelial cells (MLE-15), which have many structural and biochemical features of alveolar type II cells. We found that MLE-15 cells produce laminin-␣5, a trace amount of laminin-␣3, laminins-␤1 and -␥1, type IV collagen, and perlecan. Transforming growth factor-␤1 significantly induces expression of laminin-␣1. When grown on a fibroblast-embedded collagen gel, MLE-15 cells assemble a basement membrane-like layer containing laminin-␣5. These findings indicate that MLE-15 cells will be useful in modeling basement membrane production and assembly by alveolar type II cells. murine alveolar type II cells; transforming growth factor-␤; extracellular matrix BASEMENT MEMBRANES ARE THIN sheets of extracellular matrix that serve as a structural support for cells. They also act as a selective barrier to various solutes and affect cell properties, including proliferation, differentiation, adhesion, and migration (for review, see Ref. 6). The major components of basement membranes are laminins, type IV collagens, entactins (nidogens), and perlecan. Basement membrane composition varies between tissues and even in the same tissue at different stages of development. Laminins contribute to the diversity of basement membranes. Laminins are a family of heterotrimeric glycoproteins in which each member contains an ␣-, ␤-, and ␥-chain. To date, five ␣-, three ␤-, and three ␥-chains forming 15 laminin isoforms have been described. Laminin-␥3 is the most recently described (23) laminin chain and is the only laminin chain that is not found in basement membranes. In the current model, basement membrane assembly occurs via linking of a type IV collagen network with a laminin network by entactin (41).In the lung, alveolar type I and alveolar type II cells rest on a continuous basement membrane; however, the composition of the basement membrane under both cell types is not the same. Differences exist in the location of basement membrane anionic sites beneath alveolar type I and type II cells (34). Subsets of alveolar type II cells have localized interruptions or discontinuities of the basement membrane where cytoplasmic processes extend and contact or closely approximate interstitial fibroblasts and extracellular matrix, whereas the basement membrane beneath type I cells does not (5).The alveolar epithelial basement membrane is complex in composition and undergoes changes during development. Recent studies (29, 30) highlight the variability of laminin ␣-chain expression in the lung. Laminin-␣1 and -␣2 are present in fet...

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Characterization of α1, β1, and γ1 laminin subunits during rabbit fetal lung development

Cited by 27 publications

References 31 publications

Distribution of Dystroglycan in Normal Adult Mouse Tissues

Distribution of Dystroglycan in Normal Adult Mouse Tissues

Molecular Cloning of a Novel Laminin Chain, α5, and Widespread Expression in Adult Mouse Tissues

Laminin α-chain expression and basement membrane formation by MLE-15 respiratory epithelial cells

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