Characterization of β‐adrenoceptors in the syrian hamster harderian gland: Sexual differences and effects of either castration of superior cervical ganglionectomy

Pangerl, Andreas; Pangerl, Brigitte; Buzzell, Gerald R.; Jones, David J.; Reíter, Russel J.

doi:10.1002/jnr.490220411

Cited by 17 publications

(12 citation statements)

References 25 publications

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“…The reason for the difference in binding densities be tween sexes remains unknown. Gender-asso ciated differences in the density of p-receptors [27], gene expression [6], and in the activities of different enzymes [28] have also been found in the harderian gland of the Syrian hamster.…”

Section: Binding Of [12sl]melatonin To the Harderian Glands Of The Symentioning

confidence: 97%

Melatonin Binding Sites in the Harderian Gland of the Rat and Syrian Hamster

Guerrero

Menéndez-Peláez²,

Calvo³

et al. 1994

Neurosignals

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Specific melatonin binding sites in the harderian gland of both rat and Syrian hamster were studied using [¹²⁵I]melatonin. In both species, binding of [¹²⁵I]melatonin by harderian gland membranes exhibited properties such as dependence on time, temperature, membrane concentration, saturability, and high specificity. Only one class of high-affinity binding sites was found with a Kd of 0.19 and 6.47 nM for the rat and Syrian hamster, respectively. The binding capacity in the rat harderian gland was 4.00 fmol/mg protein; in the Syrian hamster it was 7.58 fmol/mg protein. In the rat, no sex differences were found in the binding of the tracer to the membranes. However, in the Syrian hamster, binding of [¹²⁵I]melatonin by the harderian gland was twice higher in the female than in the male. No changes were found in the Kd values (6.47 vs. 6.94 nM), while binding capacity was significantly increased in the female (13.50 fmol/mg protein) when compared to the male hamster (7.58 fmol/mg protein). Binding of [¹²⁵I]melatonin by the harderian gland of male hamsters was modified by castration but not by melatonin treatment. Castration induced an increase of binding up to the level of females. However, chronic melatonin administration did not alter the [¹²⁵I]melatonin binding in either intact or gonadectomized male hamsters. Binding studies also showed diurnal variations. There was a diurnal rhythm of [¹²⁵I]melatonin binding by Syrian hamster harderian glands with the peak at the end of the light period and the trough late in the dark period. This rhythm in the binding is observed in both male and female hamsters, although binding in females was always higher than that in males. Serum melatonin levels also demonstrated a diurnal variation with a well-established nocturnal peak of melatonin at 4.00 h and a fall after light onset at 6.00 h. As it has been previously described, nocturnal levels of serum melatonin in the male Syrian hamster were higher than those of the female hamster. Results confirm the presence of melatonin binding sites in the harderian gland of rats and Syrian hamsters, suggesting that these binding sites may mediate, in addition to other effects, the regulation of melatonin in the harderian gland.

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Section: Binding Of [12sl]melatonin To the Harderian Glands Of The Symentioning

confidence: 97%

Melatonin Binding Sites in the Harderian Gland of the Rat and Syrian Hamster

Guerrero

Menéndez-Peláez²,

Calvo³

et al. 1994

Neurosignals

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“…Our results indicate a greater [3-receptor number and affinity in males for the extraorbital lacrimal gland, and a higher [3-receptor number but lower affinity in females for the Harderian gland (Pangerl et al, 1989). The effect of sex steroids on J3-adrenergic receptors seems to differ from tissue to tissue and is probably closely related to the function of the organ.…”

Section: Discussionmentioning

confidence: 52%

“…Furthermore we recently reported a sexual dimorphism of 13-adrenoceptors in the intraorbital Harderian gland of Syrian hamsters (Pangerl et al, 1989). Whereas cz-adrenoceptors in the lacrimal gland have been characterized with binding studies as al-type receptors (Mauduit et al, 1986), [3-adrenoceptors have not been investigated.…”

Section: Introductionmentioning

confidence: 97%

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?-Adrenoceptors in the extraorbital lacrimal gland of the Syrian hamster characterization with [125I]-iodopindolol and evidence of sexual dimorphism

Pangerl

Jones

et al. 1989

J. Neural Transmission

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Saturation and competition experiments with the radiolabeled beta-adrenergic antagonist (--)-[125I]-iodopindolol were used to characterized beta-adrenoceptor density (Bmax) and receptor affinity in the extraorbital lacrimal gland of male and female Syrian hamsters. Specific binding to the receptor was saturable. Scatchard analysis of saturation isotherms revealed a single population of receptor sites. Male glands had a significantly higher Bmax (38.9 +/- 5.0 vs. 23.3 +/- 2.1 fmol/mg protein, means +/- SEM, p less than 0.02) and receptor affinity (expressed in a lower dissociation constant Kd: 0.065 +/- 0.013 vs. 0.120 +/- 0.015 nM, p less than 0.02) than female glands. Binding of the radiolabeled ligand in competition with various adrenergic antagonists showed the receptor to be stereospecific and of the beta 2-subtype.

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“…It has been reported that castration in the male rat results in an increase in the β-adrenoceptor in the pituitary gland and cerebral cortex (Petrovic et al 1984), and estrogen administration has been shown to increase β-adrenoceptor binding in fetal lung (Moawad et al 1985). In the golden hamster the female gland has a higher binding value of [ 125 I]-iodopindolol binding to β-adrenoceptor than in the male and castration in males increases the binding value to female levels (Pangerl et al 1989). Androgen and estrogen receptors have been demonstrated in the Harderian gland of the golden hamster (Vilchis and Perez-Palacios 1989).…”

Section: Discussionmentioning

confidence: 96%

Sexual differences and effects of castration on secretory mode and intracellular calcium ion dynamics of golden hamster Harderian gland

Gesase

Satoh

2001

Cell and Tissue Research

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The aim of the present work was to study the sexual differences in secretory mechanisms and intracellular calcium ion dynamics in the Harderian gland of the golden hamster. In both sexes the Harderian gland consisted of small and large lobes. In the intact control male glands the secretory portions of both lobes showed wide lumina that contained secretory material and cytoplasmic fragments, suggestive of the occurrence of exocytosis and apocrine secretion. After perfusion with HEPES-buffered Ringer's solution containing 10 microM carbamylcholine (CCh), the glandular cells showed features of enhanced secretion and a rise in intracellular calcium concentration ([Ca2+]i). In the intact control female gland the lumina of most secretory portions in the large lobe contained porphyrin accretions, and exocytosis was the sole secretory mechanism. Stimulation of the large lobe with 10 microM CCh did not raise [Ca2+]i or cause enhanced secretion. The small lobe in females resembled the male gland in secretory functions, and CCh administration caused enhanced secretion and a rise in [Ca2+]i. Castration in males abolished apocrine secretion; exocytosis became the sole secretory mechanism, and stimulation of the glandular cells with CCh did not cause enhanced secretion or induce a rise in [Ca2+]i. To the contrary, in females, castration restored apocrine secretion and CCh administration caused enhanced secretion and a rise in [Ca2+]i. Castration did not affect the secretory mechanisms and the effect of CCh on the glandular cells in the small lobes of both male and female glands. The present study points to the possibility that sex hormones may control the functioning or expression of muscarinic receptors in the Harderian gland of the golden hamster.

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Characterization of β‐adrenoceptors in the syrian hamster harderian gland: Sexual differences and effects of either castration of superior cervical ganglionectomy

Cited by 17 publications

References 25 publications

Melatonin Binding Sites in the Harderian Gland of the Rat and Syrian Hamster

Melatonin Binding Sites in the Harderian Gland of the Rat and Syrian Hamster

?-Adrenoceptors in the extraorbital lacrimal gland of the Syrian hamster characterization with [125I]-iodopindolol and evidence of sexual dimorphism

Sexual differences and effects of castration on secretory mode and intracellular calcium ion dynamics of golden hamster Harderian gland

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