Characterization of γδ T cell subsets in organ transplantation

Puig-Pey, I.; Bohne, Felix; Benítez, Carlos; López, Marta; Martínez‐Llordella, Marc; Oppenheimer, Federico; Lozano, Juan José; Abraldes, Juan G.; Tisone, Giuseppe; Rimola, Antoni; Sánchez‐Fueyo, Alberto

doi:10.1111/j.1432-2277.2010.01095.x

Cited by 64 publications

(52 citation statements)

References 33 publications

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“…17 Consistently, in vivo V␦2 neg ␥␦ T-cell expansion is associated with both the resolution of HCMV infection, which is suggestive of their antiviral function, 18 and with protection against cancer in KTRs. 19 The tight association between HCMV infection and long-lasting TEMRA V␦2 neg ␥␦ T-cell expansion has recently been confirmed by others in allogeneic hematopoietic stem cell transplantation, 20 in immunodeficient children, 21 neonates, 22 liver transplant recipients, 23 and by us in immunocompetent healthy volunteers. 15 This lifelong expansion of oligoclonal V␦2 neg ␥␦ T cells in HCMV-experienced individuals is reminiscent of the accumulation with age of oligoclonal HCMVspecific ␣␤ T cells that has been associated with immunosenescence and so-called "memory inflation."…”

Section: Introductionmentioning

confidence: 69%

Antibody-dependent anti-cytomegalovirus activity of human γδ T cells expressing CD16 (FcγRIIIa)

Couzi

Pitard

Sicard

et al. 2012

Blood

122

116

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Human cytomegalovirus (HCMV) infection is an important cause of morbidity and mortality in transplant recipients. Long-term protective immunity against HCMV requires both sustained specific T-cell response and neutralizing IgG production, but the interplay between these effector arms remains poorly defined. We previously demonstrated that ␥␦ T cells play a substantial role as anti-HCMV Tcell effectors. The observation that CD16 (Fc␥RIIIA) was specifically expressed by the majority of HCMV-induced ␥␦ T cells prompted us to investigate their cooperation with anti-HCMV IgG. We found that CD16 could stimulate ␥␦ T cells independently of T-cell receptor (TCR) engagement and provide them with an intrinsic antibody-dependent cell-mediated cytotoxic ( IntroductionHuman cytomegalovirus (HCMV) is a widespread herpesvirus with an average seroprevalence of around 50% that increases with age and low socioeconomic status. Primary HCMV infection in an immunocompetent host is asymptomatic, but the virus establishes lifelong latency probably associated with periodic reactivation episodes. Conversely, damaging clinical symptoms can be observed in the course of HCMV infection in fetuses, neonates, and immunocompromised patients, such as those infected with HIV, recipients of solid organ allografts, or undergoing allogeneic hematopoietic stem cell transplantation. Constant immune surveillance is thus critical to keep the virus in check, and actually HCMV is highly immunogenic and elicits all the arsenal of the host immune defense. 1 The early events associated with virus entry into host cells first trigger a robust production of type I IFN and inflammatory cytokines, such as IL12, which are critical for the recruitment and activation of innate immune cells, particularly phagocytic leukocytes. 2 The activated innate immune cells then initiate the development of a vigorous adaptive immune response that culminates with the production of neutralizing antiviral antibodies (Abs) and IFN␥-producing and/or cytotoxic CD8 T cells. Both of these effectors are required for the establishment of long-lasting immunity against HCMV reactivation, superinfection, and congenital infection, but the cooperation between humoral immunity and T-cell effectors remains to be clarified.Humoral response against HCMV is characterized by the production of neutralizing Abs directed against viral envelope glycoproteins (mostly gB and gH) and gene products of the UL131A-128 locus, which are involved in virus attachment and entry into host cells. 3 However, the majority (90%) of HCMVspecific Abs do not have virus-neutralizing activity. 4 Such Abs could cooperate with cell effectors expressing CD16, the low affinity Fc receptor for IgG (FcR␥IIIa), to generate an Abdependent cell-mediated cytotoxicity (ADCC). However, ADCC would require that Abs are directed against HCMV-infected cells, something that has never been reported. Natural killer (NK) cells are usually considered as the main effector of ADCC, but evidence for a role of this process in immune defense again...

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Section: Introductionmentioning

confidence: 69%

Antibody-dependent anti-cytomegalovirus activity of human γδ T cells expressing CD16 (FcγRIIIa)

Couzi

Pitard

Sicard

et al. 2012

Blood

122

116

View full text Add to dashboard Cite

show abstract

“…x We read with interest the manuscript published by Puig-Pey et al entitled ''Characterisation of gammadelta T cells in organ transplantation'' [1]. For a better reading of this paper, we would like to add several comments on the basis of our experience in cd T cell immunomonitoring in kidney transplant recipients (KTR), to enlighten their role in the context of cytomegalovirus (CMV) infection.…”

mentioning

confidence: 99%

Gamma-delta T cell expansion is closely associated with cytomegalovirus infection in all solid organ transplant recipients

Couzi

Lafarge

Pitard

et al. 2011

Transplant International

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“…Vδ1 γδT cells, which are predominant in the spleen, intestine, and liver, possess regulatory and effector properties, but Vδ2 γδT cells, which are the main subset of γδT cells in the peripheral blood, possess defense properties against pathogens [7] . Several studies have reported that a preponderance of Vδ1 γδT cells is associated with liver allograft acceptance [14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

Differences in Peripheral Blood Lymphocytes between Brand-Name and Generic Tacrolimus Used in Stable Liver Transplant Recipients

Kim

Kwon²,

Joh³

et al. 2017

Med Princ Pract

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Objectives: In this study, peripheral blood lymphocytes were compared between a brand-name and a generic tacrolimus group in stable liver transplant recipients. Subjects and Methods: Sixteen patients who underwent ABO-compatible living donor liver transplants between 2012 and 2013 and had stable graft function were included in this study. Ten patients received brand-name tacrolimus and 6 patients received generic tacrolimus. CD3, CD4, CD8, γδ, CD4+FoxP3+, and CD3-CD56+ T cells were analyzed in peripheral blood obtained preoperatively and 4, 8, 12, and 24 weeks after liver transplantation. Categorical variables were compared using a χ² test or Fisher exact test, and continuous variables were compared using the Mann-Whitney U test. Results: Regarding the baseline and perioperative characteristics, there were no statistically significant differences between the 2 groups. Immunosuppression also was not different.Subtype analysis of T-cell populations carried out in parallel showed similar levels of CD3, CD4, CD8, and γδT cells with brand-name tacrolimus and generic tacrolimus in stable liver transplant recipients. However, the levels of CD4+Foxp3+ and CD3-CD56+ T cells were higher in the brand-name tacrolimus group than in the generic tacrolimus group 8 weeks after transplantation (p < 0.05). Conclusions: The level of CD4+Foxp3+ T cells was higher in the brand-name tacrolimus group than in the generic tacrolimus group after transplantation. This finding showed that brand-name tacrolimus could have more potential immunosuppressive activity than generic tacrolimus regarding the contribution of CD4+Foxp3+ T cells to graft tolerance in liver transplant recipients.

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Characterization of γδ T cell subsets in organ transplantation

Cited by 64 publications

References 33 publications

Antibody-dependent anti-cytomegalovirus activity of human γδ T cells expressing CD16 (FcγRIIIa)

Antibody-dependent anti-cytomegalovirus activity of human γδ T cells expressing CD16 (FcγRIIIa)

Gamma-delta T cell expansion is closely associated with cytomegalovirus infection in all solid organ transplant recipients

Differences in Peripheral Blood Lymphocytes between Brand-Name and Generic Tacrolimus Used in Stable Liver Transplant Recipients

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