Characterization ofO-trimethylsilyl oximes of disaccharides by gas chromatography-mass spectrometry

Abstract

SummaryTwenty-three disaccharides have been analysed by GC-MS as their O-trimethylsilyl oximes. The relationships bel',,veen their mass spectral fragmentation and their structural characteristics have been studied in order to use GC-MS information for quahtative purposes. The presence of some structural features, such as the 1 -2 or 1 -6 linkages, can be estimated from the relative intensity of fragment ions. The method has been applied to the determination of the components present in the disaccharide fraction obtained from lactose treatment with 13-galactosidase from K. fragilis.

Gas chromatographic–mass spectrometric characterisation of tri- and tetrasaccharides in honey

“…1) linkages (Brokl et al, 2009;Sanz, Sanz, & Martínez-Castro, 2002). This peak could therefore correspond to a trisaccharide derived from a,b-trehalose substituted with glucose in 3 or 4 position; the low retention of trehaloses also supports this assignation.…”

Metabolic Fluxes in an Illuminated Arabidopsis Rosette

“…Analytes were manually identified using the TagFinder plug-in of the TagFinder software (Luedemann et al, 2008) and the reference library mass spectra and retention indices housed in the Golm Metabolome Database (http://gmd.mpimp-golm.mpg.de; Kopka et al, 2005). Interpretation of gas chromatography-electron impact fragments derived in GC-TOF-MS analysis were performed based on previously published detailed EI fragmentation pattern of trimethylsilylated and methoxiaminated carbohydrates (DeJongh et al, 1969;Laine and Sweeley, 1973;MacLeod et al, 2001;Sanz et al, 2002) and amino acids (Abramson et al, 1974;Leimer et al, 1977). Furthermore, interpretation was supported by the mass shifts observed upon in vivo 13 C labeling (Huege et al, 2007).…”

GC-EI-TOF-MS analysis of in vivo carbon-partitioning into soluble metabolite pools of higher plants by monitoring isotope dilution after 13CO2 labelling

“…1). This interpretation effort was based on the detailed EI fragmentation patterns of trimethylsilylated and methoxiaminated carbohydrates (DeJongh et al, 1969;Laine and Sweeley, 1973;MacLeod et al, 2001;Sanz et al, 2002) or amino acids (Abramson et al, 1974;Bergströ m et al, 1970;Leimer et al, 1977), which have been previously published. Furthermore, interpretation was supported by the mass shifts observed upon in vivo 13 C-labelling ( Fig.…”