Characterization, structure, and function of extracellular polymeric substances (EPS) of microbial biofilm in biological wastewater treatment systems: a review

Salama, Youssef; Chennaoui, Mohammed; Sylla, Aboubacar; Mountadar, M.; Rihani, Mohammed; Assobhei, Omar

doi:10.1080/19443994.2015.1077739

Cited by 125 publications

(36 citation statements)

References 142 publications

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“…Biofilm-forming bacteria mainly synthesize EPS. The biosynthesized EPS have numerous functions, including, mature biofilm structure, microcolony maintenance, and enhanced resistance to various disinfectants and to environmental stress ( Salama et al, 2016 , Costa et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Characterization of biofilm formed by multidrug resistant Pseudomonas aeruginosa DC-17 isolated from dental caries

Farraj

Rajaselvam

et al. 2020

Saudi Journal of Biological Sciences

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The present work reports with the screening of biofilm-producing bacteria from the dental caries. The dental pathogens showed resistance against various antibiotics and biofilm forming ability at various levels. Among the bacterial strain, Pseudomonas aeruginosa DC-17 showed enhanced biofilm production. Extracellular polymeric substance (EPS) was synthesized by the selected bacterial isolate considerably and contributed as the major component of biofilm. EPS composed of eDNA, proteins and lipids. The total protein content of the EPS was found to be 1.928 mg/mL and was the major component than carbohydrate and DNA. Carbohydrate content was 162.3 mg/L and DNA content of EPS was 4.95 μg/mL. These macromolecules interacted in the matrix to develop dynamic and specific interactions to signalling biofilm to differentiating various environments. Also, the isolated bacteria showed resistant against various commercially available antibiotics. The isolates showed more resistance against penicillin (98%) and were sensitive against amoxicillin. Among the factors, temperature, pH and sugar concentration influenced biofilm formation. Biofilm forming ability of the selected bacterial stain was tested at various pH values and alkaline pH was favoured for biofilm production. Biofilm production was found to be maximum at 40 °C and 8% sucrose enhanced biofilm formation. Biofilm formed by P. aeruginosa DC-17 was resistant against various tested antimicrobials and chemicals.

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Section: Resultsmentioning

confidence: 99%

Characterization of biofilm formed by multidrug resistant Pseudomonas aeruginosa DC-17 isolated from dental caries

Farraj

Rajaselvam

et al. 2020

Saudi Journal of Biological Sciences

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“…Different methods that have been reported are divided into three categories that is, physical method, chemical method, and combination of physical and chemical methods . Physical method includes the extraction of exopolysaccharides by using physical forces that is, by centrifugation, filtration, dialysis, and sonication technique , also by cation exchange and by heating . While chemical method includes the use of different chemicals that is, formaldehyde (H 2 CO), ethylenediaminetetraacetic acid (EDTA), sodium hydroxide (NaOH), and ethanol (C 2 H 5 OH) .…”

Section: Bacterial Exopolysaccharidesmentioning

confidence: 99%

Exopolysaccharides producing rhizobacteria and their role in plant growth and drought tolerance

et al. 2018

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Drought stress not only effect the population and activities of microorganisms inhabiting the rhizosphere but also various physiological and biochemical process in plants that is, photosynthesis, respiration, translocation, uptake of ions, carbohydrates, and nutrient metabolism. Plant growth promoting rhizobacteria (PGPR) and their exopolysaccharides (EPS) showed profound effects on plant growth and drought tolerance. Reactions of bacteria to drought stress at various organizational levels are different which depends on intensity of stress, duration, species, and growth stage. PGPR could be effectively utilized in developing strategies to facilitate water conservation strategies of plants. They have the ability to improve plant growth directly by enhancing level of phytohormones, siderophore, biofilm, and exopolysaccharides production and by increasing the nutrient availability in the rhizosphere or indirectly by protecting plants from pathogen attack. EPS producing bacteria are capable to maintain higher soil moisture content and growth of plants even under severe dried sandy soils. The evidence of survival of rhizobacteria under low moisture content obtained from the fact of rhizobacterial occurrence in the soil of desert and effective nodule formation in desert soil. Beside this, EPS produced by PGPR form rhizosheath around the roots and thus protect the plant roots from desiccation for a longer period of time. Important role exhibited by exopolysaccharides includes, protection from desiccation, microbial aggregation, plant-microbe interaction, surface attachment, bioremediation and its use by many industries for stabilizing, thickening, coagulating, gelling, suspending and for film forming. Plants inoculated with EPS-producing bacteria showed higher accumulation of proline, sugars, and free amino acids under water deficit stress. Biofilms formed by the PGPR around the roots are made up of bacterial populations or bacterial communities that encased inside the polymeric extracellular matrix formed by bacteria itself, they adhered to the external surfaces that contain sufficient moisture. It is concluded that the application of PGPR in combination with their EPS is a promising measure to combat drought stress thus, increasing global food security.

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“…Both could be released during algal activity (Li et al 2016;Yamashita and Tanoue 2003), and were good substrates for bacteria (Wetzel 2001;Pérez and Sommaruga 2006). Moreover, bacteria also release the extracellular polymeric substances (EPS) such as carbohydrates and proteins (Elliott et al 2006;Salama et al 2015). Because none of the factors were significantly correlated with the FI…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Influence of parasitic chytrids on the quantity and quality of algal dissolved organic matter (AOM)

et al. 2018

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Algae-derived dissolved organic matter (AOM) is an important nutrient source for heterotrophic bacteria, while AOM such as humic substances pose significant challenges during water treatment processing. We hypothesized that the parasitic infection of algae could change the composition and concentration of AOM. This study investigated the quality and quantity of DOM and bacterial abundance in diatom (Synedra) cultures, with and without parasitic fungi (chytrids). The quality of DOM was analyzed using three-dimensional excitation-emission matrix combined with parallel factor analysis (EEM-PARAFAC) and was compared to changes in algal and bacterial cell numbers. Bacterial abundance was higher and dissolved organic carbon concentrations were lower in the diatom cultures infected with parasitic fungi. Among the DOM compounds, the concentrations of tryptophan-like material derived from algae were significantly lower and the concentrations of humic substance-like material were higher in the infected treatment. The parasitic fungi may have consumed tryptophan-like material and stimulated the release of humic substances. These results provide the first evidence that fungal infection may modulate algal-bacterial interactions, which are associated with changes in the nature of AOM.

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Characterization, structure, and function of extracellular polymeric substances (EPS) of microbial biofilm in biological wastewater treatment systems: a review

Cited by 125 publications

References 142 publications

Characterization of biofilm formed by multidrug resistant Pseudomonas aeruginosa DC-17 isolated from dental caries

Characterization of biofilm formed by multidrug resistant Pseudomonas aeruginosa DC-17 isolated from dental caries

Exopolysaccharides producing rhizobacteria and their role in plant growth and drought tolerance

Influence of parasitic chytrids on the quantity and quality of algal dissolved organic matter (AOM)

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