Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst. roots

Bo, Surina; Mu, Dan; Li, Wenxi; Zhang, Ping

doi:10.1016/j.ijbiomac.2018.11.238

Cited by 21 publications

(11 citation statements)

References 30 publications

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“…Determination of the average-molecular weight of the MHHP was performed by high performance gel permeation chromatography (HPGPC) according to the previous mentioned method. 34 The calibration curve was plotted by Dextran standards with different molecular weights.…”

Section: Methodsmentioning

confidence: 99%

“…Cell viability was calculated as the previous study. 34 Cell viability differences were compared using paired Student's t -test.…”

Section: Methodsmentioning

confidence: 99%

“…Cell culture was prepared as mentioned previous study. 34 RAW 264.7 cells were seeded in 96-well plates (1 × 10 6 cells per well) and were incubated with MHHP polysaccharide at different concentrations (5, 40 and 200 μg mL −1 ) for 48 h. The cells treated with the medium alone were used as blank control. The cells treated with 2.5 μg mL −1 LPS (Melone-pharma, Daliang, China) were considered as a positive control.…”

Section: Methodsmentioning

confidence: 99%

“…The preparation of spleen cell was processed using the above method. 34 2.9.1.2 Effect of MHHP on spleen lymphocyte proliferation. 100 mL of the lymphocyte cell suspension (5 Â 10 6 cells per mL) was seeded in a 96-well plate using RPMI 1640 medium.…”

Section: Scanning Electron Microscopementioning

confidence: 99%

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Physicochemical properties, immunostimulatory and antioxidant activities of a novel polysaccharide isolated from Mirabilis himalaica (Edgew) Heim

Han

et al. 2022

RSC Adv.

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Section: Methodsmentioning

confidence: 99%

“…Cell viability was calculated as the previous study. 34 Cell viability differences were compared using paired Student's t -test.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Scanning Electron Microscopementioning

confidence: 99%

See 2 more Smart Citations

Physicochemical properties, immunostimulatory and antioxidant activities of a novel polysaccharide isolated from Mirabilis himalaica (Edgew) Heim

Han

et al. 2022

RSC Adv.

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“…Arnebia euchroma (Royle) Johnst (A. euchroma) is a kind of perennial herb of the arnebiaceae genus belong to boraginaceae family. This plant is recorded for the first time in Shennongbencaojing [1,2] and is widely distributed in Indian, Japan and other Asian countries, especially the arid regions of Asia and North Africa and it was widely used in China [3][4][5][6]. According to the Chinese Pharmacopoeia (2015 edition), decoction pieces of A. euchroma can be used in clearing heat and cooling blood, promoting blood circulation and detoxication, eliminating eruption and plaques.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three naphthoquinones from Arnebia euchroma (Royle) Johnst in beagle plasma by UPLC-MS/MS and application for pharmacokinetics study

Wang,

Zhang,

Zhu

et al. 2022

AChrom

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A rapid, simple and efficient ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method was established to simultaneous determination of shikonin, isobutyryl shikonin, β, βʹ-dimethylacryl alkanin in beagle plasma and evaluated by using esculetin as internal standard. The electrospray ionization (ESI) source was operated in negative ionization mode. Multi-reaction monitoring (MRM) was used to quantitatively analyzed shikonin m/z 287.0 → 217.9, isobutyryl shikonin m/z 357.0 → 268.9, β, βʹ-dimethylacryl alkanin m/z 370.0 → 270.1 and esculetin m/z 177.0 → 89.0, respectively. The method was validated for selectivity, linearity, lower limit of quantification, precision, accuracy, recovery, matrix effect and stability. All validation parameters met the acceptance criteria according to regulatory guidelines. This method was successfully applied for the pharmacokinetic study of shikonin, isobutyryl shikonin, β, βʹ-dimethylacryl alkanin in beagle dogs plasma after oral administration of A. euchroma extract.

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Isolation, structure identification, and immunostimulatory effects in vitro and in vivo of polysaccharides from Onosma hookeri Clarke var. longiforum Duthie

Aga

Liang

et al. 2022

J Sci Food Agric

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BACKGROUND This study characterized an acidic polysaccharide (OHC‐LDPA) isolated from the medicinal and edible homologous plant Onosma hookeri Clarke var. longiforum Duthie. The structure of OHC‐LDPA was elucidated based on the analysis of infrared, one‑/two‐dimensional nuclear magnetic resonance, and gas chromatography–mass spectrometry data. The immunostimulatory effects of OHC‐LDPA were identified by both in vitro and in vivo models. RESULTS The structure of OHC‐LDPA was elucidated as a typical pectin polysaccharide, consisting of galacturonic acid, galactose, arabinose, and rhamnose as the primary sugars, with linear galacturonic acid as the main chain and arabinogalacturonic acid as the main branched components. OHC‐LDPA could significantly stimulate the proliferation and phagocytosis of RAW264.7 macrophages and the release of nitric oxide in vitro. Also, it could accelerate the recovery of spleen and thymus indexes, enhance the splenic lymphocyte proliferation responses, and restore the levels of interleukin‐2, interleukin‐10, interferon‐γ, and immunoglobulin G in the serum in a cyclophosphamide‐induced immunosuppressed‐mice model. In addition, OHC‐LDPA could restore the intestinal mucosal immunity and reduce the inflammatory damage. CONCLUSION OHC‐LDPA could improve the immunity both in vitro and in vivo and could be used as a potential immunostimulant agent. © 2022 Society of Chemical Industry.

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Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst. roots

Cited by 21 publications

References 30 publications

Physicochemical properties, immunostimulatory and antioxidant activities of a novel polysaccharide isolated from Mirabilis himalaica (Edgew) Heim

Physicochemical properties, immunostimulatory and antioxidant activities of a novel polysaccharide isolated from Mirabilis himalaica (Edgew) Heim

Simultaneous determination of three naphthoquinones from Arnebia euchroma (Royle) Johnst in beagle plasma by UPLC-MS/MS and application for pharmacokinetics study

Isolation, structure identification, and immunostimulatory effects in vitro and in vivo of polysaccharides from Onosma hookeri Clarke var. longiforum Duthie

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